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Stem Cell Media Selection Guide

Stem Cell Media Selection Guide

Which stem cell culture medium is right for you?

Medium	NutriStem® hPSC XF Medium				NutriStem® hPSC XF Medium, Modified GF-Free	MSC NutriStem® XF Medium
Cell Type	Human PSCs	Human PSCs	Human PSCs	Human PSCs	Human PSCs	Human MSCs	Human MSCs
Application	Feeder-Dependent hPSC culture	Feeder-Free hPSC culture	Translational hPSC culture	Translational hPSC culture, single cell	Reprogramming	Completely Defined Translational hMSC culture	Translational hMSC culture
Additional Supplement	None	None	None	None	bFGF (20 ng/mL)	MSC NutriStem® Supplement Mix	PLTMax® Human Platelet Lysate
Matrix	MEFs/HFFs	Matrigel Cultrex Geltrex Other	Vitronectin LaminStem™ 521 Laminin-511	LaminStem™ 521	LaminStem™ 521 Matrigel Laminin-511	MSC Attachment Solution	None
Xeno-Free	Possible (HFFs)	No	Yes	Yes	Yes	Yes	Yes
Weekend-Free Feeding*	No	Yes	Yes	Yes	n/a	n/a	n/a
Dissociation Reagent	Collagenase IV	Dispase Collagenase IV Accutase 0.5mM EDTA	0.5mM EDTA Accutase	Trypsin-EDTA Accutase	Trypsin-EDTA Accutase	Recombinant Trypsin Solution	Recombinant Trypsin Solution
Freezing Media	NutriFreez® D10 Cryopreservation Medium	NutriFreez® D10 Cryopreservation Medium	NutriFreez® D10 Cryopreservation Medium	NutriFreez® D10 Cryopreservation Medium	NutriFreez® D10 Cryopreservation Medium	NutriFreez® D10 Cryopreservation Medium	NutriFreez® D10 Cryopreservation Medium
Additional Reagents	Gelatin, Human Serum Albumin (HSA), Soybean Trypsin Inhibitor, EZ-PCR™ Mycoplasma Detection Kit, DPBS, Aquaguard™-1 Solution, Aquaguard™-2 Solution for Water Baths

*Weekend-free feeding is possible, though with any hPSC culture, optimal growth is seen with daily feeding. It is not recommended to skip feedings on a routine or long-term basis.