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Cryopreservation

A powerful cryopreservation solution

Biological Industries’ NutriFreez® brand of cryopreservation media has set the standard for high-quality freezing solutions. NutriFreez® D10 Medium is the first fully defined, optimized freezing solution designed and validated for the cryopreservation of various cell types, including hESCs, iPSCs, PBMCs, and hMSCs. NutriFreez® D10 Medium maintains animal component-free conditions during cryopreservation, essential to maintaining consistency when culturing cells in a xeno-free system. With proven reliability and consistency, NutriFreez® D10 Medium provides a high-performance protective environment for cells during the freezing, storage, and thawing process.

  • Optimized for maximum cell recovery and protection
  • Unique, serum-free and fully defined formulation
  • Widely referenced, manufactured under cGMP


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High Viability

Viability comparison of Human PBMCs show >91% viability in NutriFreez® D10 Medium when compared to cells cryopreserved in homebrew freezing solutions.

Superior Attachment

Attachment comparison of various cell lines following cryopreservation in NutriFreez® D10 Medium when compared to homebrew freezing solutions.

Superior Recovery

Obtain more human MSCs in less time with a >7-fold cell increase following cryopreservation in NutriFreez™ D10 Medium when compared to other commercially available solutions.

 



Application Spotlight

Septic Shock patients studied by researchers from the Ottawa Hospital Research Institute

Mesenchymal stem cells (MSCs) have been shown to exert important immunomodulatory effects in both acute and chronic diseases. In acute inflammatory conditions such as septic shock, immunomodulatory cell therapy must be administered within hours of diagnosis; therefore a cryopreserved, allogeneic cell product that can be thawed prior to infusion to the patient is best suited for this purpose. The objective of this study is to determine an optimal cryopreservant that can preserve the viability of MSCs after thawing.

Results:Cell recovery and attachment compared to homebrew and serum-free competitor freezing solutions, primary human mesenchymal stem cells (from healthy donors) cryopreserved in NutriFreez® D10 Cryopreservation Medium exhibited the best post-thaw viability and recovery rates in addition to increased cell attachment and growth performance.

Figure: A. Comparison of cell recovery over homebrew and competitor freezing solutions at 6 days post thaw. B. Comparison of cell viability over homebrew and competitor freezing solutions by Trypan blue exclusion and Annexin V/PI staining FACS analysis (direct post thaw).

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Reference: Salkhordeh, et. al. May 2018. Evaluation of different cryopreservation agents for mesenchymal stem cell as final study product. Cytotherapy.

 



 


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  • NutriFreez® D10 Cryopreservation Medium

    6 Review(s)
    • A chemically defined, animal component-free, protein-free cryopreservation solution
    • High recovery post thaw
    • cGMP-manufactured
    • Validated with various cell types and applications
    • Drug Master Files available

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