Vitronectin ACF (Human Recombinant), 200 µg

Recombinant lyophilized protein for the attachment of human pluripotent stem cells.
SKU: 05-754-0002

Availability: In stock

$60.00
  • Buy 2 for $55.00 each and save 9%

Description

Details

Product Overview

Vitronectin is a secreted glycoprotein that can support cell adhesion through binding to various integrins and proteoglycans. Recombinant vitronectin ACF can function as a chemically-defined matrix component for the attachment of human embryonic and induced pluripotent stem cells in a feeder-free culture system. Recombinant Vitronectin ACF is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Vitronectin ACF is 52.2 kDa.

Specifications

Specifications

QTY 200 µg
Storage Conditions -20° to -80°C
Instructions for Use

Preparation of Vitronectin ACF, 0.5mg/ml

Reconstitution procedure should be performed on ice.
  • Spin down the vial before reconstitution.
  • To the original 0.2mg vial add 0.4ml of sterile tissue culture water. DO NOT VORTEX.
  • Incubate on ice for a few minutes.
  • Gently mix by pipetting up and down.
  • Keep on ice for immediate use (up to 1 week at 2-8°C)
For long-term storage (3 months) aliquot and freeze at (-80)°C. Additional freeze-thaw cycles are not recommended.

Documentation

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Reviews

Customer Reviews (1)

Vitronectin ACF: easy to use and great for generating hiPS cellsReview by Dr. Christina Warnecke
Quality
We used Vitronectin ACF for the generation of human induced pluripotent stem cells from human primary skin fibroblasts after transfection with plasmids expressing the Yamanaka factors.
I stored the reconstituted solution in 60 µl aliquots at -80°C. With one aliquot diluted in 12 ml PBS, you can pre-coat at least two 6-well plates, so 200 µg are sufficient for at least 13 6-well plates. The protocols are provided with the substance. To keep the procedure short, you can also directly add the substance to the cells/medium (which is useful when you have to split the cells urgently e.g. on a weekend), but I routinely pre-coated the plates for 1 h. The fibroblasts were efficiently reprogrammed to stem cells and the stem cells grew and looked very well on Vitronectin. On recombinant laminin 511 E8 fragment, which I also tried for comparison, the stem cells seemed to adhere more tightly than on Vitronectin ACF, but this is rather a disadvantage, since the cells should be passaged with EDTA and mechanical disloging (generating lumps but not single cells). So for routine culture and passaging, I prefer Vitronectin ACF. I already re-ordered it several times. (Posted on 4/20/2020)

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