MSC NutriStem® XF Medium

  • Defined, xeno-free, serum-free medium

  • Designed for optimal growth and expansion of human MSCs

  • Customizable formulation

  • Custom scale-up services available

  • Scientific and regulatory support

  • Drug Master Files available

Name SKU Size Price Qty
MSC NutriStem® XF Medium (Basal Medium + Supplement) 05-200-1A-KT 500 mL
MSC NutriStem® XF Basal Medium 05-200-1A 500 mL
MSC NutriStem® XF Basal Medium, without Phenol Red 05-202-1A 500 mL
MSC NutriStem® XF Supplement Mix 05-201-1U 3 mL



Product Overview:

Faster growth rates and stronger cell yields

MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium supports long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential.

hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other commercial serum-free media.  In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles, and karyotypic stability over long-term culture. 

Key advantages

  • Serum-free, xeno-free, defined culture system
  • Superior cell growth compared to serum-containing media
  • Supports long-term growth and self-renewal of hMSCs from multiple sources
  • Consistent, reliable MSC cultures
  • Suitable for the harvest of high quality exosomes (EVs) for future clinical applications
  • Eliminates the need to spend time and money pre-qualifying FBS lots
  • Manufactured under cGMP
  • FDA Drug Master File (DMF) available

Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.

hMSC-BM were cultured in MSC NutriStem® XF

Figure: NutriStem® MSC XF Medium promotes superior proliferation and expansion of hMSCs over time as compared to other serum-free and serum-containing media.

Sample Data

Initial isolation

Figure: Human mesenchymal stem cells were initially isolated from Wharton's jelly (left image) and placenta (right image) using MSC NutriStem™ XF on pre-coated plates with MSC Attachment Solution (cat. no. 05-752-1). High number of hMSC was obtained.


Figure: MSC NutriStem® XF promotes excellent proliferation of hMSC from a variety of sources while maintaining normal fibroblast-like, spindle shape cell morphology.


Self-renewal potential

Figure: hMSC-BM and hMSC-AT were expanded in MSC NutriStem® XF for 3-5 passages prior to 14 days of CFU-F assay. Colonies were stained with 0.5% crystal violet (x100). The cells maintain their self-renewal potential.

Differentiation potential

Figure: hMSC-BM (top panel) and hMSC-AT (bottom panel) expanded in MSC NutriStem® XF for 3-5 passages prior to trilineage differentiation. Representative images of stained adipocytes (Oil Red O), osteocytes (Alizarin Red) and chondrocytes (Alcian Blue). The cells maintain their multilineage differentiation potential.



Form Liquid
Brand NutriStem®
Quality Control MSC NutriStem® XF Medium is application-tested and validated for optimal maintenance and expansion of undifferentiated hMSCs, while maintaining their multi-lineage differentiation potential.
Specifications The complete mesenchymal stem cell culture media consists of two components: MSC NutriStem® XF Basal Medium (05-200-1) and MSC NutriStem® XF Supplement Mix (05-201-1).
Instructions for Use

Storage and Stability

  • MSC NutriStem® XF Basal Medium (05-200-1) should be stored at 2 to 8°C. 
  • MSC NutriStem® XF Supplement Mix (05-201-1) should be stored at -20°C. Avoid freeze/thaw cycles.
  • The complete MSC NutriStem® XF Medium is stable at 2 to 8°C for up to 30 days.
  • Avoid exposure to light.  

Medium Preparation

  • Thaw frozen MSC NutriStem® XF Supplement Mix at at 2 to 8°C or room temperature.
  • MSC NutriStem® XF Basal Medium contains L-glutmine.
  • To prepare 100 mL of complete medium:  aseptically add 0.6 mL of MSC NutriStem® XF Supplement Mix to 100 mL of MSC NutriStem® XF Basal Medium.
  • To prepare 500 mL of complete medium:  aseptically add 3 mL of MSC NutriStem® XF Supplement Mix to 500 mL of MSC NutriStem® XF Basal Medium.
  • Store complete MSC NutriStem® XF Medium at 2 to 8°C protected from light for up to 30 days.
Legal MSC NutriStem® XF Basal Medium is registered as an in-vitro diagnostic (IVD) medical device.

A Drug Master File (DMF) for MSC NutriStem® XF Medium is available.



Clinical Trials

  1. L.A McIntyre et. al. Cellular Immunotherapy for Septic Shock. A Phase I Clinical Trial. American Journal of Respiratory and Critical Care Medicine, Vol. 197, No. 3, 2018
  2. K. Schlosser et al. Effects of Mesenchymal Stem Cell Treatment on Systemic Cytokine Levels in a Phase 1 Dose Escalation Safety Trial of Septic Shock Patients. Critical Care Medicine, doi: 10.1097/CCM.0000000000003657


  3. V. Alonso-Camino and B. Mirsch, Rapid expansion of Mesenchymal Stem/Stromal Cells using optimized media supplemented with human platelet lysate PLTMax® or PLTGold®, suitable for cGMP expansion at large scale. Cytotherapy, Volume 21, Issue 5, Supplement, May 2019, Page S8
  4. S. Pang et al. DISSECTING THE MOLECULAR PATHWAYS OF APOPTOSIS IN MESENCHYMAL STROMAL CELL THERAPY. Cytotherapy, Volume 21, Issue 5, Supplement, May 2019, Page S85
  5. M. Valitsky et al. Cerebrospinal Fluid (CSF) Exchange with Artificial CSF Enriched with Mesenchymal Stem Cell Secretions Ameliorates Experimental Autoimmune Encephalomyelitis. Int. J. Mol. Sci. 2019, 20(7), 1793;
  6. K. Narbute et al. Intranasal Administration of Extracellular Vesicles Derived from Human Teeth Stem Cells Improve Motor Symptoms and Normalize Tyrosine Hydroxylase Expression in the Substantia Nigra and Striatum of the 6-Hydroxydopamine-Treated Rats. STEM CELLS TRANSLATIONAL MEDICINE, pp. 1-10, 2019
  7. U. Jonavičė et al. Extracellular vesicles can act as a potent immunomodulators of human microglial cells. Journal of Tissue Engineering and Regenerative Medicine, 2019
  8. J.W Li et al. Mesenchymal stromal cells-derived exosomes alleviate ischemia/reperfusion injury in mouse lung by transporting anti-apoptotic miR-21-5pEuropean Journal of Pharmacology, 2019
  9. Z. Zhu et al. Exosomes derived from human umbilical cord mesenchymal stem cells accelerate growth of VK2 vaginal epithelial cells through MicroRNAs in vitro. Human Reproduction, dey344,, 2018
  10. A. Yamasaki et al. Osteochondral regeneration using constructs of mesenchymal stem cells made by bio three‐dimensional printing in mini‐pigs. Journal of Orthopaedic Research, 2018
  11. Vu N.B., Le P.TB., Truong N.C., Van Pham P. (2018) Off-the-Shelf Mesenchymal Stem Cell Technology. In: Pham P. (eds) Stem Cell Drugs - A New Generation of Biopharmaceuticals. Stem Cells in Clinical Applications. Springer, Cham
  12. J.K. Ledwon et al. Osteogenic Differentiation Of Msc As A Model Study Of The Postnatal Progressive Crouzon Syndrome. Plastic and Reconstructive Surgery - Global Open. 6(4S):116, APR 2018
  13. D.Lisini et al. Adipose tissue-derived mesenchymal stromal cells for clinical application: An efficient isolation approach. Current Research in Translational Medicine,
  14. T.C. Peak et al. Exosomes secreted by placental stem cells selectively inhibit growth of aggressive prostate cancer cells. Biochemical and Biophysical Research Communications, Volume 499, Issue 4, 23 May 2018, Pages 1004-1010

  1. S. Khan et al. cGMP-Compatible Large-Scale Production of Mesenchymal Stem Cells (MSCs) In Xeno- and Serum-Free Media for Allogeneic Cell Therapies. Cytotherapy,Volume 20, Issue 5, Supplement, Page S42, 2018
  2. A.C. Volz and P.J. Kluger, Completely serum-free and chemically defined adipocyte development and maintenance.Cytotheraoy, April 2018 Volume 20, Issue 4, Pages 576–588
  3. C. Elseberg et al. The Challenge of Human Mesenchymal Stromal Cell Expansion: Current and Prospective Answers. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech.
  4. C. Ceccaldi et al. Optimization of Injectable Thermosensitive Scaffolds with Enhanced Mechanical Properties for Cell Therapy. Macromolecular Bioscience, 2017
  5. D. Boruczkowski et al.Third-party Wharton’s jelly mesenchymal stem cells for treatment of steroid-resistant acute and chronic graft-versus-host disease: a report of 10 cases. Turkish Journal of Biology, 40: 493-500, 2016
  6. Jianxia H. et al. Long term effect and safety of Wharton's jelly-derived mesenchymal stem cells on type 2 diabetes. Experimental and Therapeutic Medicine, Volume 12 Issue 3, 2016
  7. S. Bobis-Wozowicz et al. Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles. Journal of Molecular Medicine, 2016
  8. L. Berger et al. Tumor Specific Recruitment and Reprogramming of Mesenchymal Stem Cells in TumorigenesisSTEM CELLS Volume 34, Issue 4, Version of Record online: 31 DEC 2015
  9. K.Y. Tan et al. Serum-free media formulations are cell line–specific and require optimization for microcarrier culture. Cytotherapy, 2015 
  10. Cai, Zhen, et al. Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia. Aesthetic plastic surgery 39.3 (2015): 431-439.
  11. A. Jarmalavičiūtė et al., Exosomes from dental pulp stem cells rescue human dopaminergic neurons from 6-hydroxy-dopamine–induced apoptosis. Cytotherapy, 2015
  12. M.Pokrywczynska et al., Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins. Archivum Immunologiae et Therapiae Experimentalis, May 2015
  13. U Pivoraitė et. al., Exosomes from Human Dental Pulp Stem Cells Suppress Carrageenan-Induced Acute Inflammation in Mice. Inflammation, April 2015
  14. S.H. Mei, et al. Isolation and large-scale expansion of bone marrow-derived mesenchymal stem cells with serum-free media under GMP-compliance. Cytotherapy, Volume 16, Issue 4, Supplement , Page S111, April 2014
  15. Mira Genser-Nir et al. Toward a serum-free, xeno-free culture system for optimal growth and expansion of hMSC suited to therapeutic applications. From 23rd European Society for Animal Cell Technology (ESACT), Meeting: Better Cells for Better Health.Lille, France. 23-26 June 2013
  16. McVey, Mark John, et al. Microparticles as biomarkers of lung disease-enumeration in biological fluids using lipid bilayer microspheres. American Journal of Physiology-Lung Cellular and Molecular Physiology (2016): ajplung-00369.
  17. Y. Lopez, et al. Identification of Optimal Conditions For Generating MSCs For Preclinical Testing: Comparison of Three Commercial Serum-Free Media And Low-Serum Growth Medium. From 18th ISCT Annual Meeting, Seattle, USA, 2012.
  18. R. D. Foster et al. GorodetskyFibrin microbeads loaded with mesenchymal cells support their long-term survival while sealed at room temperature.Tissue Eng Part C Methods2011TISSUE ENGINEERING: Part C, Volume 17, Number 7, 2011


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Customer Reviews (3)

my cells love it!Review by Tony
Added platelet lysate and my cells really took off and look great. (Posted on 5/22/2018)
very efficientReview by Yasmin
We found this product to be very useful for growing MSCs, especially by means of reserving their properties. (Posted on 1/16/2018)
excellentReview by Jason
outstanding performance for my MSC cells, both morphology and proliferation (Posted on 8/5/2016)

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