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EndoGo™ XF Medium

Defined, xeno-free, serum-free medium for long-term expansion of large and small vessels of endothelial cells

Name SKU Size
EndoGo™ XF Medium 05-400-1A-KT 500 mL



Product Overview:

EndoGo™ XF Medium is a novel xeno-free culture medium specially designed for long-term expansion of large and small vessels endothelial cells from various sources. The medium provides optimally balanced nutritional environment that selectively promotes proliferation of normal human endothelial cells , while maintaining typical cobblestone-like cell morphology, phenotypic surface marker profile, and angiogenic differentiation potential.
EndoGo™ XF supports human macro and microvascular endothelial cells (MVEC).


  • Defined, xeno-free, serum-free medium
  • Supports long-term expansion of large and small vessels endothelial cells
  • Maintains high proliferation potential, typical morphology and EC marker expression

HDMEC (Human Dermal Microvascular Endothelial Cells) proliferation

HDMEC endothelial cells proliferation in EndoGo XF Media

HPMEC (Human Pulmonary Microvascular Endothelial Cells) proliferation

HPMEC endothelial cells proliferation in EndoGo XF Media

Cell counts and population doubling level (PDL) of HDMEC and HPMEC expanded for several passages in EndoGo™ XF in comparison to commercial FBS-containing medium. Viable cells were counted using ChemoMetec Viability and Cell Count Assay.
*HPMEC did not survive P5 in the FBS-containing medium.

Macro vascular HUVEC (Human Umbilical Vein Endothelial Cells)

Micro vascular HDMEC (Human Dermal Microvascular Endothelial Cells)

Microvascular endothalial cells and macrovascular endothelial cells maintain classic cobblestone like morphology after expansion for several sequential passages with equal seeding (5000 cells/cm2) in EndoGo™ XF +2% OTC human AB serum on hFN pre-coated dishes (A), expanded cells preserved endothelial cell features (EC markers expression) (B) and angiogenic potential to form capillary-like tubes (C).



QTY 500 mL
Instructions for Use

The complete endothelial cell culture media consist of two components: EndoGo™ XF Medium (05-400-1) and EndoGo™ XF Supplement Mix (05-410-1). Individual components are not sold separately. In addition, the medium required the use of human AB serum (off the clot) or human platelet lysate (not supplied with the media).
EndoGo™ XF Medium is recommended for use in conjunction with Recombinant Trypsin Solution with EDTA (Cat. No. 03-079-1) and Soybean Trypsin Inhibitor (SBTI) (Cat. No. 03-048-1).

Storage and Stability

  • EndoGo™ XF Medium should be stored at -20°C. 
  • EndoGo™ XF Supplement Mix should be stored at -20°C. Avoid repeated freeze/thaw cycles (up to two freeze – thaw cycles max.)
  • The complete EndoGo™ XF Medium is stable at 2 to 8°C for up to 30 days.
  • Avoid exposure to light.  

Medium Preparation

For the preparation of the complete medium, aseptically add 2.5ml of EndoGo™ XF Supplement Mix to 500ml of EndoGo™ XF Medium. Alternatively, for the preparation of smaller volumes, EndoGo™ Supplement Mix may be thawed, aliquoted and re-freezed.
Add 2-5% of male human AB serum (off the clot) (e.g. Access Biologicals) or pooled human platelet lysate (e.g. Cook Medicals, Stemulate™ G34934).



  1. A.Ogunye et. al., Ex vivo expansion of cord blood-derived endothelial cells using a novel xeno-free culture media National Center for Biotechnology Information, U.S. National Library of Medicine, 2019 Mar 22;5(5):FSO376. doi: 10.2144/fsoa-2018-0103
  2. V. Alonso-Camino and W. Mirsch, In vitro expansion of human primary endothelial cells for clinical use using EndoGo™ XF Medium supplemented with PLTGold® human platelet lysate. Cytotherapy, May 2018Volume 20, Issue 5, Supplement, Page S87


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