Cell Therapy and Stem Cell Research
Mesenchymal Stem Cell Culture Under SF, XF Conditions - Useful Tips
How to isolate mesenchymal stem cells from tissue, what reagents to use while seeding the mesenchymal stem cells, and how to sub-culture mesenchymal stem cells under serum-free, xeno-free culture conditions.
A scientific poster presents the development of novel serum-free, xeno-free media, and supplements that efficiently differentiate hMSCs from various sources into adipocytes, osteoblasts, and chondrocytes. Molecular characterization and functional assays were used to evaluate the quality and purity of the differentiated cells.
Poster: Cell Therapy Compliant Xeno-Free Culture System for Human Endothelial Cells
The poster evaluated a novel XF culture system comprising EndoGo™ XF medium and auxiliary solutions for attachment, dissociation, and cryopreservation of human endothelial cells. Results show that EndoGo™ XF medium and auxiliary solutions provide a novel hEC XF culture system enabling the expansion of hEC suitable for cell therapy applications.
Poster: hMSC Serum-Free, Xeno-Free Culture System
A scientific poster that presents a novel Serum-Free, Xeno-Free culture system for human Mesenchymal Stem Cells, suitable for therapeutic applications. The system was developed and evaluated by BI's R&D department.
Technical Resources: Assuring Multipotency of Human Mesenchymal Stem Cells (hMSC)
Read here about phenotypic and functional assessments which are used to assist in successful purification and correct identification of hMSC identity
Technical Resources: iPSC-derived Cardiomyocytes
iPSC-derived cardiomyocytes (iPSC-CMs) are considered ideal for disease modeling and clinical applications as they possess a robust proliferation capacity. Read here a brief overview of iPSC-CM differentiation, disease modeling, and potential clinical applications
Technical Resources: Passaging Methods for hPSCs under Feeder-Free Conditions
Technical review of a variety of hPSC dissociation methodologies: large aggregates, small aggregates, and single cell
Technical Resources: Assuring Pluripotency of ESC and iPSC Lines
Read here a short review of the methods which are used to confirm pluripotency in newly established and existing pluripotent stem cell lines
Application Note: Novel Precoating-Free Protocol for Culturing hPSC using Vitronectin
A user-friendly, timesaving protocol that has been developed to eliminate the traditional vitronectin coating procedure for the culture of hPSC
Single-cell passaging is a cell culture method that is compatible with desired downstream applications such as gene editing or CRISPR/Cas9. Read here how the combination of xeno-free media, recombinant protein matrices, and recombinant trypsin solutions, enables single-cell passaging without the addition of ROCK inhibitor
SnapShot: Directed Differentiation of ESCs and iPSCs
Pluripotent stem cells have the ability to differentiate into the hundreds of cell types within the body. Download this illustrated poster which highlights some of the strategies for directing the differentiation of ESCs and iPSCs into defined cell types
Infographic: Stem Cells in Regenerative Medicine
Learn about the challenges of producing therapy-worthy cells, the need for regenerative medicine, and the future regenerative medicine options
Cryopreservation of Cells
Serum-free Freezing Medium - Performance Validation
Read here why it is important to cryopreserve cells in a serum-free freezing medium while using a medium free of serum in the cell culture.
Fetal Bovine Serum
Introduction to Fetal Bovine Serum Class
Everything you wanted to know about fetal bovine serum in cell culture: Which grades of FBS are available? Which tests are performed for Quality Control?
Classical Cell Culture
Read here instructions for preparation of single-strength medium.
Read here instructions for preparation of powdered medium.
Mycoplasma Contamination
Mycoplasma: Effective Detection and Treatment
Read here how to use PCR for the detection of mycoplasma in cell cultures and what are the methods for treating contaminated cultures.
10 Quick Tips to Prevent Mycoplasma Contamination
Follow these quick, easy tips to prevent cell culture contamination
Guide for Antibiotics Selection
Read here a guide for antibiotics selection and appropriate concentrations
Serum-Free Media
Adaptation of Adherent Cells to Serum-Free Media
In order to succeed in the serum-free growth of adherent cells, it is necessary to follow proper procedures carefully. Read here detailed instructions and techniques.
Serum-Free Medium for Animal Cell Culture
A thorough technical guide for the use of serum-free media and supplements in animal cell culture in suspension
