Tech Tips & Posters

Cell Therapy and Stem Cell Research

How to isolate mesenchymal stem cells from tissue, what reagents to use while seeding the mesenchymal stem cells, and how to sub-culture mesenchymal stem cells under serum-free, xeno-free culture conditions.

This poster presents NutriStem® V9 XF - a superior pluripotent stem cell culture medium, optimized for vitronectin

A scientific poster presents the development of novel serum-free, xeno-free media and supplements that efficiently differentiate hMSCs from various sources into adipocytes, osteoblasts, and chondrocytes. Molecular characterization and functional assays were used to evaluate the quality and purity of the differentiated cells.

The poster evaluated a novel XF culture system comprising EndoGo™ XF medium and auxiliary solutions for attachment, dissociation, and cryopreservation of human endothelial cells. Results show that EndoGo™ XF medium and auxiliary solutions provide a novel hEC XF culture system enabling the expansion of hEC suitable for cell therapy applications.

A scientific poster that presents a novel Serum-Free, Xeno-Free culture system for human Mesenchymal Stem Cells, suitable for therapeutic applications. The system was developed and evaluated by BI's R&D department.

Read here about phenotypic and functional assessments which are used to assist in successful purification and correct identification of hMSC identity

iPSC-derived cardiomyocytes (iPSC-CMs) are considered ideal for disease modeling and clinical applications as they possess a robust proliferation capacity. Read here a brief overview of iPSC-CM differentiation, disease modeling, and potential clinical applications

Technical review of a variety of hPSC dissociation methodologies: large aggregates , small aggregates and single cell

Read here a short review of the methods which are used to confirm pluripotency in newly established and existing pluripotent stem cell lines

A user-friendly, timesaving protocol that has been developed to eliminate the traditional vitronectin coating procedure for culture of hPSC

Single cell passaging is a cell culture method which is compatible with desired downstream applications such as gene editing or CRISPR/Cas9. Read here how the combination of xeno-free media, recombinant protein matrices and recombinant trypsin solutions, enables single cell passaging without the addition of ROCK inhibitor

Pluripotent stem cells have the ability to differentiate into the hundreds of cell types within the body. Download this illustrated poster which highlight some of strategies for directing the differentiation of ESCs and iPSCs into defined cell types

Learn about the challenges of producing therapy-worthy cells, the need for regenerative medicine and the future regenerative medicine options

Cryopreservation of Cells

Read here why it is important to cryopreserve cells in a serum-free freezing medium while using medium free of serum in the cell culture.

Fetal Bovine Serum

Everything you wanted to know about fetal bovine serum in cell culture: What grades of FBS are available? What tests are performed for Quality Control?

Classical Cell Culture

Read here instructions for preparation of single-strength medium.

Read here instructions for preparation of powdered medium.

Mycoplasma Contamination

Read here how to use PCR for the detection of mycoplasma in cell cultures and what are the methods for treating contaminated cultures.

Follow these quick, easy tips to prevent cell culture contamination

Read here a guide for antibiotics selection and appropriate concentrations

Mycoplasma contamination can lead to loss of time and money, invalid scientific data, misleading or retracted publication, loss of cell lines and contamination between labs. Learn more with this infographic

Serum-Free Media

In order to succeed in the serum-free growth of adherent cells, it is necessary to follow proper procedures carefully. Read here detailed  instructions and techniques.

A thorough technical guide for the use of serum-free media and supplement in animal cell culture in suspension