Cell Therapy and Stem Cell Research
Mesenchymal Stem Cell Culture Under SF, XF Conditions - Useful TipsHow to isolate mesenchymal stem cells from tissue, what reagents to use while seeding the mesenchymal stem cells, and how to sub-culture mesenchymal stem cells under serum-free, xeno-free culture conditions.
Poster: Novel xeno-free, serum-free vitronectin-based culture system for hPSCThis poster presents NutriStem® V9 XF - a superior pluripotent stem cell culture medium, optimized for vitronectin
Poster: A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue SourcesA scientific poster presents the development of novel serum-free, xeno-free media and supplements that efficiently differentiate hMSCs from various sources into adipocytes, osteoblasts, and chondrocytes. Molecular characterization and functional assays were used to evaluate the quality and purity of the differentiated cells.
Poster: Cell Therapy Compliant Xeno-Free Culture System for Human Endothelial CellsThe poster evaluated a novel XF culture system comprising EndoGo™ XF medium and auxiliary solutions for attachment, dissociation, and cryopreservation of human endothelial cells. Results show that EndoGo™ XF medium and auxiliary solutions provide a novel hEC XF culture system enabling the expansion of hEC suitable for cell therapy applications.
Poster: hMSC Serum-Free, Xeno-Free Culture SystemA scientific poster that presents a novel Serum-Free, Xeno-Free culture system for human Mesenchymal Stem Cells, suitable for therapeutic applications. The system was developed and evaluated by BI's R&D department.
Technical Resources: Assuring Multipotency of Human Mesenchymal Stem Cells (hMSC)Read here about phenotypic and functional assessments which are used to assist in successful purification and correct identification of hMSC identity
Technical Resources: iPSC-derived CardiomyocytesiPSC-derived cardiomyocytes (iPSC-CMs) are considered ideal for disease modeling and clinical applications as they possess a robust proliferation capacity. Read here a brief overview of iPSC-CM differentiation, disease modeling, and potential clinical applications
Technical Resources: Passaging Methods for hPSCs under Feeder-Free ConditionsTechnical review of a variety of hPSC dissociation methodologies: large aggregates , small aggregates and single cell
Technical Resources: Assuring Pluripotency of ESC and iPSC LinesRead here a short review of the methods which are used to confirm pluripotency in newly established and existing pluripotent stem cell lines
Application Note: Novel Precoating-Free Protocol for Culturing hPSC using VitronectinA user-friendly, timesaving protocol that has been developed to eliminate the traditional vitronectin coating procedure for culture of hPSC
Application Note: Single Cell Passaging with NutriStem® hPSC XF on Laminin-521 without the Need for ROCK InhibitorSingle cell passaging is a cell culture method which is compatible with desired downstream applications such as gene editing or CRISPR/Cas9. Read here how the combination of xeno-free media, recombinant protein matrices and recombinant trypsin solutions, enables single cell passaging without the addition of ROCK inhibitor
SnapShot: Directed Differentiation of ESCs and iPSCsPluripotent stem cells have the ability to differentiate into the hundreds of cell types within the body. Download this illustrated poster which highlight some of strategies for directing the differentiation of ESCs and iPSCs into defined cell types
Infographic: Stem Cells in Regenerative MedicineLearn about the challenges of producing therapy-worthy cells, the need for regenerative medicine and the future regenerative medicine options
Cryopreservation of Cells
Serum-free Freezing Medium - Performance ValidationRead here why it is important to cryopreserve cells in a serum-free freezing medium while using medium free of serum in the cell culture.
Fetal Bovine Serum
Introduction to Fetal Bovine Serum ClassEverything you wanted to know about fetal bovine serum in cell culture: Which grades of FBS are available? Which tests are performed for Quality Control?
Classical Cell Culture
Single-strength MediumRead here instructions for preparation of single-strength medium.
Powdered MediumRead here instructions for preparation of powdered medium.
Mycoplasma Contamination
Mycoplasma: Effective Detection and TreatmentRead here how to use PCR for the detection of mycoplasma in cell cultures and what are the methods for treating contaminated cultures.
10 Quick Tips to Prevent Mycoplasma ContaminationFollow these quick, easy tips to prevent cell culture contamination
Guide for Antibiotics SelectionRead here a guide for antibiotics selection and appropriate concentrations
Serum-Free Media
Adaptation of Adherent Cells to Serum-Free MediaIn order to succeed in the serum-free growth of adherent cells, it is necessary to follow proper procedures carefully. Read here detailed instructions and techniques.
Serum-Free Medium for Animal Cell CultureA thorough technical guide for the use of serum-free media and supplement in animal cell culture in suspension