The use of serum-free media brings many advantages including: batch to batch uniformity, consistency in prices, freedom from concern regarding mycoplasma and other contaminants, as well as simplified product purification and downstream processing.
BI's serum-free media and supplement have been proven to be effective for a wide variety of applications such as:
DCCM-2: The same as DCCM-1 but with low protein formulation. Glutamine must be added prior to use.
LPM (BSA-free): This formulation is free of Bovine Serum Albumin. Despite this very low protein content, LPM has proven to be remarkably effective for the growth of a wide variety of hybridomas and other lymphocytes.
Glutamine must be added prior to use.
BI's serum-free media and supplement have been proven to be effective for a wide variety of applications such as:
- Culture of murine myeloma cells (NS-1, NS-0/1, SP2)
- Culture of mouse-mouse hybridoma cells (from NS-1, NS-0/1, SP2, X63-Aag8-653, BW-S147)
- Culture of rat-mouse, rat-rat, and human-human hybridomas
- Monoclonal antibody production
- Culture of other human, murine and avian lymphocytes
- Viral production (such as HEV virus from Turkey B-lymphoblastiod cell lines)
- Culture of various anchorage-dependent cells (such as human osteosarcoma, human epithelial, and thyroid cells)
- Maintenance of most anchorage-dependent cells during long production periods
DCCM-2: The same as DCCM-1 but with low protein formulation. Glutamine must be added prior to use.
LPM (BSA-free): This formulation is free of Bovine Serum Albumin. Despite this very low protein content, LPM has proven to be remarkably effective for the growth of a wide variety of hybridomas and other lymphocytes.
Glutamine must be added prior to use.
Growth of Hybridoma 3D2 C2 (mouse-mouse from Myeloma X63-Ag 8-653)
Growth of Hybridoma 4-15 (mouse-mouse from Meyloma NS-1Ag 4.1)
Cell Adaptation to serum-free medium
For many cell types no adaptation procedures are necessary and may even be detrimental. In other cases standard-weaning procedures may be necessary.Sequential adaption
- Subculture the cells growing in serum-supplemented medium into 1:1 (v/v) mixture of serum-free medim and serum supplemented medium.
- When the cell density is >5x105 cells/ml, subculture the cells in an equal volume of SFM at a cell density 2.5x105 to 3x105 cells/ml.
- Continue to subculture after the cell density is >5x105 cells/ml in serum-free medium until the serum is 0.1% with about 85% cell viability.
- Subculture the cells into SFM with an inoculum of 2.5x105 to 3x105 cells/ml.
- When the cell density is 1x106 to 3x106 cells/ml (4 to 6 days post planting) subculture the cells again.
- Stock cultures of serum-free medium adapted cells should be subcultured is serum-free medium every 3 to 5 days when the cell density is 1x106 cells/ml with 90% viability.