Description
Details
Product Overview
LaminStem™ 521 is a defined matrix for feeder-free culture of human embryonic (hES) and induced pluripotent (hiPS) stem cells.
When used with NutriStem® hPSC XF Medium, LaminStem™ 521 has been proven to promote cellular survival and expansion of hES and hiPS cells after plating from single cell suspension. When cultured with LaminStem™ 521, the hES and hiPS cells grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation.
Features
- Defined matrix
- Superior to Matrigel for feeder-free culture of hES and hiPS cells using NutriStem® hPSC XF Medium
- No treatment with apoptosis inhibitors needed
- Support long-term self-renewal of human pluripotent stem cells (hPSC) and neural stem cells (NSCs)
Sample Data
Recent studies have shown that superior clonal derivation of hES cell lines is possible with the combined use of NutriStem® hPSC XF Medium and LaminStem™ 521.

Figure 1. H1 hESC were cultured with LaminStem™ 521 using NutriStem® hPSC XF Medium (day 3).
Specifications
Specifications
QTY | 1 mL |
---|---|
Form | Liquid |
Brand | LaminStem™ |
Storage Conditions | Store at -20ºC - -80ºC |
Shipping Conditions | Dry Ice |
Specifications | 100 µg/mL |
Instructions for Use | General Coating Procedure
Important Notes
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Legal | LaminSem™ is manufactured by BioLamina AB. |
References
references
- A. Reyes, et al. 2015. Xeno-Free and Defined Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Functionally Integrate in a Large-Eyed Preclinical Model Plaza. Stem Cell Reports.
- Rodin S, et al. 2014. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun. 5:3195. doi: 10.1038/ncomms4195.
Documentation
Material Safety Data Sheet
Manuals and Protocols
Product Literature
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.