Recombinant Trypsin EDTA Solution

Animal component-free cell dissociation solution

Defined alternative to porcine trypsin

Name SKU Size Price Qty
Recombinant Trypsin EDTA Solution 03-079-1B 100 mL
Recombinant Trypsin EDTA Solution 03-079-1C 20 mL



Product Overview

Recombinant Trypsin EDTA Solution is a defined, animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues.  The EDTA component accelerates the dissociation action of the trypsin enzyme. This solution is optimized for sensitive cells, such as primary human mesenchymal stem cells (hMSC).

The Recombinant Trypsin EDTA Solution is a pure enzyme solution, which maximizes the yield of functionally viable cells from culture vessels, while preventing toxicity induced by other contaminating proteases. This solution does not contain undesirable proteins such as carboxypeptidase A and chymotrypsin, which are commonly found in porcine or bovine-derived trypsin solutions.  In addition, Recombinant Trypsin EDTA Solution eliminates the risk of viruses and other potential adventitious agents that can be carried in animal-derived components. 



  • Defined, animal component-free 
  • Ready-to-use solution
  • Accelerated enzymatic action
  • Pure, recombinant trypsin - no contaminating proteases
  • Optimized for sensitive cells, such as hMSCs
  • Compatable with cells cultured in both serum-free and serum-containing media



Storage Conditions Room temperature
Form Liquid
  • Source:
    Produced by submerged microbial fermentation. This solution is animal component-free, and derived from a production process that does not utilize any raw materials and/or processing aids of animal origin.
  • Storage & Stability:
    Do not store or use past the expiration date
  • Quality Control:
    Recombinant Trypsin Solution performance is tested on hMSC. Additional standard evaluations are pH, osmolality, and sterility tests.



  1. Hubel, A. (2018) Cryopreservation of Human Adipose Stem Cells, in Preservation of Cells: A Practical Manual, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118989883.oth4


Certificate of Analysis

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