NutriFreez™ D10 Cryopreservation Medium

  • A chemically defined, animal component-free, protein-free cryopreservation solution

  • High recovery post thaw

  • cGMP-manufactured

  • Validated with various tissue and cell types




This product replaces 05-065-1, 05-710-1 and 05-712-1

Name SKU Size Price Qty
NutriFreez™ D10 Cryopreservation Medium 05-713-1E 50 mL
$85.00
NutriFreez™ D10 Cryopreservation Medium 05-713-1B 100 mL
$145.00
NutriFreez™ D10 Cryopreservation Medium 05-713-1A 500 mL
$175.00

Description

Details

Product Overview

NutriFreez™ D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines.  When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.  

NutriFreez™ D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing.  Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez™ D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

  • Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)
  • Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
  • Human Peripheral Blood Mononuclear Cells (PBMC’s)
  • Human Endothelial Cells (EC)
  • T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)
  • Neuron Cells
  • Hybridomas
  • CHO cells
  • Vero Cells
  • Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

 

NutriFreez™ D10 Cryopreservation Medium Features

  • Cryoprotective formulation designed to minimize dehydration effects
  • Effective maintenance of cell viability, adhesion, and bioactivity
  • Ready-to-use solution, simple protocol
  • Suitable for use in cell banks and biopharmaceutical applications
  • Animal component-free, serum-free, protein-free solution
  • DMF has been acknowledged by the FDA
  • cGMP Manufactured

Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez™ D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (Nishishita et al. Am J Stem Cells. 2015).

Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez™ D10 Cryopreservation Medium exhibit the best post-thaw viability (Salkhordeh et. al. Cytotherapy, May 2018).

* NutriFreez™ brand replaces the brand CryoStem™

H1 hESC BGO1V/hOG cels (Variant hESC hOct4-GFP reporter cells)

1 hour post-thawing

Day 1 Post-thawing

Day 4 Post-thawing

hESC were frozen in NutriFreez™ D10 Cryopreservation Medium and were thawed into NutriStem® hPSC XF on Matrigel. The cells maintain excellent attachment ability as well as growth performance.

Specifications

Specifications

Storage Conditions 2-8°C
Shipping Conditions Cold Pack
Instructions for Use

Cryopreservation of serum-free cell cultures 

  1. For freezing adherent cells, detach cells using the appropriate dissociation solution. (For freezing of cells in suspension, skip to step 2.)
  2. Centrifuge to pellet the cells at 200 - 300 x g for 3 to 5 minutes.
  3. Suspend the cell pellet in cold NutriFreez™ D10 Cryopreservation Medium at a concentration of 3 to 5 million cells/mL.
  4. Freeze the cells gradually (1 to 2°C per minute) and store them in liquid nitrogen.
  5. Viability and recovery of cryopreserved cells should be evaluated 24 hours after storage of vials in liquid nitrogen by following the thawing procedure outlined below.

 

Thawing cryopreserved cells 

  1. Thawing should be performed at 37°C.
  2. Immediately after thawing, suspend the cells in serum-free growth medium at a ratio of at least 1:10.
  3. Centrifuge and suspend in growth medium as desired.
  4. Culture the cells according to the recommended seeding density.

References

references

    Pluripotent Stem Cells
  1. G. Gagliardi et at. Characterization and Transplantation of CD73-Positive Photoreceptors Isolated from Human iPSC-Derived Retinal Organoids. Stem Cell Reports j Vol. 11 j 665–680 j September 11, 2018
  2. M. Amit, J. Itskovitz-Eldor Single cells pluripotent stem cells in a suspension culture. US Patent App. 16/055,110, 2018
  3. G. Guo, et al. Epigenetic resetting of human pluripotency." bioRxiv (2017): 146712.
  4. S. Reichman et al. Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno‐Free and Feeder‐Free Conditions.STEM CELLS 35.5 (2017): 1176-1188.
  5. F. Pistollato, et al. Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing. JoVE (Journal of Visualized Experiments) 124 (2017): e55702-e55702.
  6. L. Tian et al. Efficient and controlled generation of 2D and 3D bile duct tissue from human pluripotent stem cell-derived spheroids." Stem Cell Reviews and Reports 12.4 (2016): 500-508.
  7. J. Chal et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols 11.10 (2016): 1833-1850.
  8. M. S. Y Hiew. Generation and Microrna Expression Profiling of Colorectal Cancer Cell-Derived Induced Pluripotent Cancer Cells. Diss. UTAR, 2016.
  9. N. Nishishita et al. An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. American journal of stem cells 4.1 (2015): 38.
  10. Z. Danhong, et al. Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate.U.S. Patent Application No. 14/114,182, 2014
  11. C. Martin et al. Highly Efficient microRNA-Enhanced mRNA Reprogramming of Diseased Human Fibroblasts in a Feeder-Free Culture System. STEMGENT Application Note, 2014
  12. C. Buensuceso et al. Induced pluripotent stem cells prepared from human kidney-derived cells . US Patent 20140073049 A1, 2014
  13. R. Sivapatham, X.Zeng. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods in Molecular Biology, 2014
  14. L. Tian, N. Prasad, Y. Jang. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2014
  15. C. Buensuceso et al. Induced pluripotent stem cells from human umbilical cord tissue-derived cells. US Patent 20130157365, 2013
  16. S. Hikita et al. Methods of Culturing Retinal Pigmented Epithelium Cells, Including Xeno-Free Production, RPE Enrichment, and Cryopreservation. US Patent 20130196369 A1, 2013
  17. N. Nishishita et al. Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State. PLoS ONE 7(6): e38389. doi:10.1371/journal.pone.0038389 , 2012
  18. F. Pistollato et al. Standardization of pluripotent stem cell cultures for toxicity testing. Vol. 8, No. 2 , Pages 239-257 (doi:10.1517/17425255.2012.639763), 2012
    Mesenchymal Stem Cells
  1. M. Salkhordeh et al. Evaluation of Different Cryopreservation Agents for Mesenchymal Stem Cell as Final Study Product. Cytotherapy, Volume 20, Issue 5, Supplement, May 2018, Page S50
  2. Kundrotas, Gabrielis, et al. Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow. Oncotarget 7.10 (2016): 10788.
  3. Kundrotas, Gabrielis. Investigation of human mesenchymal stem cell genetic characteristics to ensure therapy safety. Diss. Vilnius University, 2016.

    Peripheral Blood Mononuclear Cells (PBMC)
  1. N. Milevoj et. al. A combination of electrochemotherapy, gene electrotransfer of plasmid encoding canine IL-12 and cytoreductive surgery in the treatment of canine oral malignant melanoma. Research in Veterinary Science, volume 122, February 2019, Pages 40-49

  2. Other Cell Lines
  3. A. C. Brown et al. Long-Term Culture of Nephron Progenitor Cells Ex Vivo. In: Vainio S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY
  4. E. Nigro, E. Soprana, A. Brini, et l., An Antitumor Cellular Vaccine Based on a Mini-Membrane IgE. J Immunol 188:103-110 (2012).
  5. G. Di Lullo, E. Soprana, M. Panigada, et al. The combination of marker gene swapping and fluorescence-activated cell sorting improves the efficiency of recombinant modified vaccinia virus Ankara vaccine production for human use. J. of Virological Methods, 163, Issue 2: 195–204 (2010)
  6. Amit, Michal, and Joseph Itskovitz-Eldor. Novel methods and culture media for culturing pluripotent stem cells. U.S. Patent Application 13/821,244.
  7. De Falco, Elena, et al. A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts.Cell and tissue banking 14.2 (2013): 277-287.

* The CryoStem™ brand is replaced by the brand NutriFreez™

Documentation

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.
Please enter the product lot number and click search.
If your search does not return any results, please contact us for assistance.

Reviews

Write Your Own Review

You're reviewing: NutriFreez™ D10 Cryopreservation Medium

How do you rate this product? *

  1 star 2 stars 3 stars 4 stars 5 stars
Quality

You may also be interested in the following product(s)

MSC NutriStem® XF Medium

MSC NutriStem® XF Medium

3 Review(s)

Starting at: $125.00

NutriStem® hPSC XF Medium

NutriStem® hPSC XF Medium

1 Review(s)

Starting at: $65.00

NutriStem® V9 XF Medium

NutriStem® V9 XF Medium

Starting at: $15.00