Description
Details
Product Description
IWP-2 is an inhibitor of the Wnt pathway, specifically targeting membrane-bound O-acyltransferase porcupine (PORCN) with an IC50 of 27 nM. This inhibition, in turn, prevents Wnt activation, secretion, and signaling (Garcia-Reyes, et al.), which leads to decreased self-renewal of human embryonic stem cells (ESCs) (Kim, et al). IWP-2 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into functional cardiomyocytes when combined with CHIR99021 (Cat. No. SM-0001-0010), IWP-4 (Cat. No. SM-0004-0005), Activin A, bFGF, and BMP4 (Lian, et al.). Additionally, IWP-2 shows promise in the differentiation of iPSCs into corneal epithelial cells (Mikhailova, et al.).Product Synonyms:
IWP 2, Inhibitor of WNT Production-2Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.
Specifications
Specifications
| QTY | 10 mg |
|---|---|
| Form | Powder |
| Molecular Weight | 466.60 |
| Molecular Formula | C22H18N4O2S3 |
| Chemical Name | N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2-d]pyrimidin-2-yl)thio]-acetamide |
| CAS Number | 686770-61-6 |
| Target | Porcupine (PORCN) |
| Appearance | White to off-white (Solid) |
| Purity | ≥95% by NMR |
| Solubility and Reconstitution | Soluble in DMSO up to 4 mM, for example: 10 mg/428.632 mL = 0.023 mg/mL = 0.05 mM 10 mg/85.727 mL = 0.117 mg/mL = 0.25 mM 10 mg/42.863 mL = 0.233 mg/mL = 0.5 mM 10 mg/8.573 mL = 1.166 mg/mL = 2.5 mM |
| Storage Conditions | Store at: Powder: -20°C for 3 years 4°C for 2 years In solvent: -80°C for 6 months -20°C for 1 month |
| Legal | This product is for Research Use Only and is not intended for therapeutic or diagnostic use. |
References
references
- Garcia-Reyes, et al. 2018. Discovery of Inhibitor of Wnt Production 2 (IWP-2) and Related Compounds as Selective ATP-Competitive Inhibitors of Casein Kinase 1 (CK1). J Med Chem 61(9): 4087-4102.
- Kim, et al. 2013. Modulation of β-catenin function maintains mouse epiblast stem cell and human embryonic stem cell self-renewal. Nature Communications. 4(2403).
- Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.
- Mikhailova, et al. 2014. Small-molecule induction promotes corneal epithelial cell differentiation from human induced pluripotent stem cells. Stem Cell Reports. 2(2): 219-231.
Documentation
Certificate of Analysis
Certificates of Analysis (COA's) describe quality control data for specific product lots.
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
If your search does not return any results, please contact us for assistance.
