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IWP-2

Inhibitor of WNT Production-2, 10 mg
SKU: SM-0003-0010

Availability: In stock

$125.00

Description

Details

Product Description

IWP-2 is an inhibitor of the Wnt pathway, specifically targeting membrane-bound O-acyltransferase porcupine (PORCN) with an IC50 of 27 nM. This inhibition, in turn, prevents Wnt activation, secretion, and signaling (Garcia-Reyes, et al.), which leads to decreased self-renewal of human embryonic stem cells (ESCs) (Kim, et al). IWP-2 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into functional cardiomyocytes when combined with CHIR99021 (Cat. No. SM-0001-0010), IWP-4 (Cat. No. SM-0004-0005), Activin A, bFGF, and BMP4 (Lian, et al.). Additionally, IWP-2 shows promise in the differentiation of iPSCs into corneal epithelial cells (Mikhailova, et al.).

Product Synonyms:
IWP 2, Inhibitor of WNT Production-2

Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.

Specifications

Specifications

QTY 10 mg
Form Powder
Molecular Weight 466.60
Molecular Formula C22H18N4O2S3
Chemical Name N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
CAS Number 686770-61-6
Target Porcupine (PORCN)
Appearance White to off-white (Solid)
Purity ≥95% by NMR
Solubility and Reconstitution Soluble in DMSO up to 4 mM, for example:
10 mg/428.632 mL = 0.023 mg/mL = 0.05 mM
10 mg/85.727 mL = 0.117 mg/mL = 0.25 mM
10 mg/42.863 mL = 0.233 mg/mL = 0.5 mM
10 mg/8.573 mL = 1.166 mg/mL = 2.5 mM
Storage Conditions Store at:
Powder:
-20°C for 3 years
4°C for 2 years

In solvent:
-80°C for 6 months
-20°C for 1 month
Legal This product is for Research Use Only and is not intended for therapeutic or diagnostic use.

References

references

  • Garcia-Reyes, et al. 2018. Discovery of Inhibitor of Wnt Production 2 (IWP-2) and Related Compounds as Selective ATP-Competitive Inhibitors of Casein Kinase 1 (CK1). J Med Chem 61(9): 4087-4102.
  • Kim, et al. 2013. Modulation of β-catenin function maintains mouse epiblast stem cell and human embryonic stem cell self-renewal. Nature Communications. 4(2403).
  • Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.
  • Mikhailova, et al. 2014. Small-molecule induction promotes corneal epithelial cell differentiation from human induced pluripotent stem cells. Stem Cell Reports. 2(2): 219-231.

Documentation

Materials Safety Data Sheet

Technical Data Sheet

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