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CHIR99021

Highly potent inhibitor of GSK3α and GSK3β.
SKU: SM-0001-0010

Availability: In stock

$125.00

Description

Details

Product Description

CHIR99021 is a highly-potent and -selective inhibitor of GSK3α (IC50 of 10) and GSK3β (IC50 of 6.7), exhibiting over 500-fold selectivity when compared to its nearest homologs. CHIR99021 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into human pancreatic beta cells (Pagliuca, et al.), neural progenitor cells (Li, et al. & Qi, et al.), cardiovascular progenitor cells (Cao, et al.), and functional cardiomyocytes (Lian, et al. & Burridge, et al.). Additionally, CHIR99021 has been shown to promote the induction of human PSCs (Li, W. et al.) and self-renewal of PSCS for maintenance purposes (Ying, et al.).

Product Synonyms:
CT 99021, CHIR-99021, CHIR 99021

Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.

Specifications

Specifications

QTY 10 mg
Form Powder
Molecular Weight 465.34
Molecular Formula C22H18Cl2N8
Chemical Name 6-[2-[4-(2,4-Dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)pyrimidin-2- ylamino]ethylamino]pyridine-3-carbonitrile
CAS Number 252917-06-9
Target GSK3; Autophagy
Appearance White to yellow (Solid)
Purity ≥95% by LCMS
Solubility and Reconstitution Soluble in DMSO up to 20mM, for example:
10 mg/107.448 mL = 0.093 mg/mL = 0.2 mM
10 mg/21.489 mL = 0.465 mg/mL = 1 mM
10 mg/4.298 mL = 2.330 mg/mL = 5 mM
10 mg/2.149 mL = 4.650 mg/mL = 10 mM
Storage Conditions Store at:
Powder:
-20°C for 3 years
4°C for 2 years

In solvent:
-80°C for 6 months
-20°C for 1 month
Legal This product is for Research Use Only and is not intended for therapeutic or diagnostic use.

References

references

  • Burridge, et al. 2015. Chemically defined culture and cardiomyocyte differentiation of human pluripotent stem cells. Curr Protoc Hum Genet. 87(1): 1-15.
  • Cao, et al. 2013. Highly efficient induction and long-term maintenance of multipotent cardiovascular progenitors from human pluripotent stem cells under defined conditions. 23:1119-11132.
  • Li, et al. 2011. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. PNAS. 108(20): 8299-304.
  • Li, W., et al. 2009. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 27: 2992-3000. PMID: 19839055.
  • Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.
  • Pagliuca, et al. 2014. Generation of functional human pancreatic β cells in vitro. Cell 159: 428-439.
  • Qi, et al. 2017. Combined small-molecule inhibition accelerations the derivation of functional cortical neurons from human pluripotent stem cells. Nature Biotechnology 35(2): 154-163.
  • Ying, Q., et al. 2008. The ground state of embryonic stem cell self-renewal. Nature 453: 519-523. PMID: 18497825.

Documentation

Materials Safety Data Sheet

Technical Data Sheet

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