IWP-4

Inhibitor of WNT Production-4, 5 mg
SKU: SM-0004-0005

Availability: In stock

$195.00

Description

Details

Product Description

IWP-4 is an inhibitor of the Wnt pathway, specifically targeting membrane-bound O-acyltransferase porcupine (PORCN) with an IC50 of 25 nM. IWP-4 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into functional cardiomyocytes when combined with CHIR99021 (Cat. No. SM-0001-0010), IWP-2 (Cat. No. SM-0003-0010), Activin A, bFGF, and BMP4 (Lian, et al.), as well as in suspension cultures for cardiac differentiation from human PSCs (Chen, et al.). IWP-4 precisely induces expression of myosin light chain in both atrial and ventricular forms for the creation of primitive cardiomyocytes from human embryonic stem cells (ESCs) (Hudson, et al.).

Product Synonyms:
IWP 4, Inhibitor of WNT Production-4

Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.

Specifications

Specifications

QTY 10 mg
Form Powder
Molecular Weight 496.62
Molecular Formula C23H20N4O3S3
Chemical Name N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
CAS Number 686772-17-8
Target Porcupine (PORCN)
Appearance White to off-white (Solid)
Purity ≥95% by HLPC
Solubility and Reconstitution Soluble in DMSO up to 1 mM, for example:
5 mg/1006.810 mL = 0.005 mg/mL = 0.01 mM
5 mg/201.361 mL = 0.025 mg/mL = 0.05 mM
5 mg/100.680 mL = 0.049 mg/mL = 0.1 mM
5 mg/20.136 mL = 0.248 mg/mL = 0.5 mM
Storage Conditions Store at:
Powder:
-20°C for 3 years
4°C for 2 years

In solvent:
-80°C for 6 months
-20°C for 1 month
Legal This product is for Research Use Only and is not intended for therapeutic or diagnostic use.

References

references

  • Chen, et al. 2015. Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells. Stem Cell Research. 15(2): 365-375.
  • Hudson, et al. 2011. Primitive cardiac cells from human embryonic stem cells. Stem Cells and Development. 21(9): 1513-23.
  • Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.

Documentation

Materials Safety Data Sheet

Technical Data Sheet

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