Description
Details
Product Description
IWP-4 is an inhibitor of the Wnt pathway, specifically targeting membrane-bound O-acyltransferase porcupine (PORCN) with an IC50 of 25 nM. IWP-4 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into functional cardiomyocytes when combined with CHIR99021 (Cat. No. SM-0001-0010), IWP-2 (Cat. No. SM-0003-0010), Activin A, bFGF, and BMP4 (Lian, et al.), as well as in suspension cultures for cardiac differentiation from human PSCs (Chen, et al.). IWP-4 precisely induces expression of myosin light chain in both atrial and ventricular forms for the creation of primitive cardiomyocytes from human embryonic stem cells (ESCs) (Hudson, et al.).Product Synonyms:
IWP 4, Inhibitor of WNT Production-4Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.
Specifications
Specifications
QTY | 10 mg |
---|---|
Form | Powder |
Molecular Weight | 496.62 |
Molecular Formula | C23H20N4O3S3 |
Chemical Name | N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide |
CAS Number | 686772-17-8 |
Target | Porcupine (PORCN) |
Appearance | White to off-white (Solid) |
Purity | ≥95% by HLPC |
Solubility and Reconstitution | Soluble in DMSO up to 1 mM, for example: 5 mg/1006.810 mL = 0.005 mg/mL = 0.01 mM 5 mg/201.361 mL = 0.025 mg/mL = 0.05 mM 5 mg/100.680 mL = 0.049 mg/mL = 0.1 mM 5 mg/20.136 mL = 0.248 mg/mL = 0.5 mM |
Storage Conditions | Store at: Powder: -20°C for 3 years 4°C for 2 years In solvent: -80°C for 6 months -20°C for 1 month |
Legal | This product is for Research Use Only and is not intended for therapeutic or diagnostic use. |
References
references
- Chen, et al. 2015. Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells. Stem Cell Research. 15(2): 365-375.
- Hudson, et al. 2011. Primitive cardiac cells from human embryonic stem cells. Stem Cells and Development. 21(9): 1513-23.
- Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.
Documentation
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