Description
Details
Product Overview
MSCgo™ Adipogenic Differentiation Medium is a serum-free and xeno-free formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature adipocytes. The MSCgo™ Adipogenic Differentiation Medium is validated to efficiently differentiate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC), and Wharton’s jelly (WJ-MSC). The MSCgo™ Adipogenic Differentiation Medium kit includes a basal medium and two supplement mixes, containing all growth factors and supplements necessary for a complete adipoogenic differentiation medium. No additional supplements are required.
The MSCgo™ Adipogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs. This kit enables reliable differentiation of hMSCs to mature adipocytes without background differentiation or interruption in cellular metabolism.
Features
- Serum-free, xeno-free medium
- All required growth factors and supplements included in kit
- Reliable differentiation to mature adipocytes
- Each lot is application tested
- Contains stable L-alanyl-L-glutamine
- Does not contain antibiotics
Components
- MSCgo Adipogenic Differentiation Basal Medium: 100 mL
- MSCgo Adipogenic Differentiation Supplement Mix I: 100 μL
- MSCgo Adipogenic Differentiation Supplement Mix II: 1.5 mL
Adipogenesis Results
Adipogenic differentiation of hMSC results in the formation of spherical cells accumulated with lipid droplets that can be detected using an inverted microscope. The amount of differentiated cells can be vary using different hMSC (e.g. source, age, and passage number).
After expansion in culture using MSC NitriStem XF Medium, hMSCs from adipose tissue (hMSC-AT), bone marrow (hMSC-BM), and cord tissue (hMSC-CT) were transfered to a differentiation assay in MSCgo™ Adipogenic Differentiation Medium. Images were taken after 16 days of adipogenesis and Oil Red-O staining (20x).
Specifications
Specifications
| Form | Liquid |
|---|---|
| Brand | MSCgo™ |
| Storage Conditions | Adipogenic Differentiation Basal Medium: 2 to 8°C Adipogenic Differentiation Supplement Mix I: -20°C Adipogenic Differentiation Supplement Mix II: -20°C |
| Quality Control | The MSCgo Adipogenic Differentiation Medium is validated for optimal differentiation of hMSC into chondrocytes. Additional tests are: pH, osmolality, endotoxins and sterility tests. |
| Specifications | Required Materials for Adipogenic Assay:
|
| Instructions for Use | Complete Medium Preparation
Note: The combination of both MSCgo Adipogenic Supplement Mix I and II into the Basal Medium generates a complete medium that is ready for use. No additional supplements are required. |
| Legal | For human ex vivo tissue and cell culture processing applications. This reagent is not approved for human or animal use, or for application of in vitro diagnostic procedures. |
References
references
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- V.P. Mantripragada and G.F. Muschler Improved biological performance of human cartilage-derived progenitors in platelet lysate xenofree media in comparison to fetal bovine serum media. Current Research in Translational Medicine, 2022
- S. Cai et al. Single-cell RNA sequencing reveals the potential mechanism of heterogeneity of immunomodulatory properties of foreskin and umbilical cord mesenchymal stromal cells. Cell & Bioscience volume 12, Article number: 115 (2022)
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- R. Moloudi et al. Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions. Scientific Reportsvolume 8, Article number: 12481 (2018)
- A. Mamchur et al. Adipose‐Derived Stem Cells of Blind Mole Rat Spalax Exhibit Reduced Homing Ability: Molecular Mechanisms and Potential Role in Cancer Suppression. Stem Cells, Volume36, Issue10, October 2018, Pages 1630-1642
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Documentation
Materials Safety Data Sheet
MSCgo™ Adipogenic Differentiation Medium (MSDS)- MSCgo™ Adipogenic Differentiation Supplement Mix I (MSDS)
- MSCgo™ Adipogenic Differentiation Supplement Mix II (MSDS)
Manuals and Protocols
MSCgo™ Adipogenic Differentiation Medium Instructions for UseProduct Literature
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
