MSCgo™ Chondrogenic Differentiation Medium

Complete, serum-free, xeno-free, ready-to-use kit

Optimized for directed differentiation of hMSCs to chondrocytes
Name SKU Size Price Qty
MSCgo™ Chondrogenic Differentiation Medium 05-220-1B-KT 100 mL
$195.00

Description

Details

Product Overview

MSCgo™ Chondrogenic Differentiation Medium is a serum-free (SF) and xeno-free (XF) formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature chondrocytes. The MSCgo™ Chondrogenic Differentiation Medium is validated to efficiently differeniate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC).

The MSCgo™ Chondrogenic Differentiation Medium kit includes a basal medium and supplement, containing all growth factors and supplements necessary for a complete chondrogenic differentiation medium.  No additional supplements are required.

The MSCgo™ Chondrogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs.  This kit enables reliable differentiation of hMSCs to mature chondrocytes without background differentiation or interruption in cellular metabolism.

 

Features

  • Serum-free, xeno-free medium
  • All required growth factors and supplements included in kit
  • Reliable differentiation to mature chondrocytes
  • Each lot is application tested
  • Does not contain antibiotics

 

Components

  • MSCgo Chondrogenic Differentiation Basal Medium:  100 mL
  • MSCgo Chondrogenic Differentiation Supplement Mix :  10 mL

 

Chondrogenesis Results

Chondrogenic differentiation of hMSC in 3D spheroid culture in the formation of cartilage with a typical extracellular matrix rich of Aggrecan. Aggrecan is a proteoglycan that can be used as an indicator for cartilage formation and can be detected with Alcian Blue, a dark-blue copper-containing dye that acts as an indication of mature chondrocytes. The staining intensity can be vary using different hMSC (e.g. source, age, and passage number). 

Cartilage differentiation results of hMSC from various sources after 21 day assay using MSCgo™ Chondrogenic followed by Alcian Blue staining.

Histological images of mature chondrocytes surrounded by a cartilage matrix after a 21-day differentiation assay using MSCgo™ Chondrogenic Medium followed by Toluidine blue (40x).

Specifications

Specifications

Form Liquid
Brand MSCgo™
Storage Conditions

Chondrogenic Differentiation Basal Medium:  2 to 8°C

Chondrogenic Differentiation Supplement Mix :  -20°C

Quality Control The MSCgo Chondrogenic Differentiation Kit is validated for optimal differentiation of hMSC into chondroocytes. Additional tests are: pH, osmolality, endotoxins and sterility tests.
Specifications

Required Materials for Chondrogenic Assay

  • MSCgo™ Chondrogenic Differentiation Medium (BI Cat. No. 05-220-1 and 05-221-1) 
  • 96-well, U-bottom, non-tissue culture treated plate (for suspension)
  • MSC NutriStem® XF (BI Cat. No. 05-200-1)
  • Alcian Blue 8 GX - OPTIONAL
Instructions for Use

Complete Medium Preparation

  • 1.  Thaw MSCgo™ Chondrogenic Supplement Mix (05-221-1D) at room temperature.
  • 2.  Dilute the Supplement Mix 1:10 in the MSCgo™ Chondrogenic Basal Medium (05-220-1B) by adding 10 mL of the Supplement Mix to 90 mL of Basal Medium.
  • 3.  Store the complete medium at 2 to 8°C for up to 30 days.

Note:  The combination of the MSCgo Chondrogenic Supplement Mix into the Basal Medium generates a complete medium that is ready for use.  No additional supplements are required.

Legal For human ex vivo tissue and cell culture processing applications. This reagent is not approved for human or animal use, or for application of in vitro diagnostic procedures.

References

references

  1. E. Çerci & H.Erdost, Stem Cell Identification and Clinical Practice International Journal of Agricultural and Natural Sciences E-ISSN:2651-3617 12(1): 17-19, 2019
  2. O. Ben Menachem - Zidon, et al. Systemically transplanted mesenchymal stem cells induce vascular-like structure formation in a rat model of vaginal injury plos one, June 13, 2019 https://doi.org/10.1371/journal.pone.0218081
  3. M.Y. Meng et. al. Assessment of tumor promoting effects of amniotic and umbilical cord mesenchymal stem cells in vitro and in vivo. J Cancer Res Clin Oncol (2019) 145: 1133. https://doi.org/10.1007/s00432-019-02859-6
  4. R. Moloudi et al. Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions. Scientific Reportsvolume 8, Article number: 12481 (2018)
  5. J. Leber et al., Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media. Process Biochemistry, volume 59, Part B, August 2017, Pages 255-265
  6. L. Pu et al., Compared to the amniotic membrane, Wharton’s jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration. Stem Cell Research & Therapy 2017 8:72

Documentation

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