Description
Details
Product Overview
Recombinant Trypsin Solution is a defined, animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues. This solution is optimized for sensitive cells, such as primary human mesenchymal stem cells (hMSC).
The Recombinant Trypsin Solution is a pure enzyme solution, which maximizes the yield of functionally viable cells from culture vessels, while preventing toxicity induced by other contaminating proteases. This solution does not contain undesirable proteins such as carboxypeptidase A and chymotrypsin, which are commonly found in porcine or bovine-derived trypsin solutions. In addition, Recombinant Trypsin Solution eliminates the risk of viruses and other potential adventitious agents that can be carried in animal-derived components.
Features
- Defined, animal component-free
- Ready-to-use solution
- Pure, recombinant trypsin - no contaminating proteases
- Optimized for sensitive cells, such as hMSCs
- Compatible with cells cultured in both serum-free and serum-containing media
Recombinant Trypsin Solution
Crude Trypsin EDTA Solution
Figure 1: Rocovry of hMSC-BM after dissociation with both Recombinant Trypsin Solution and the common Trypsin EDTA Solution (porcine) following re-seeding in MSC NutriStem® XF on pre-coated plates. Representative images were taken on day 5 post-dissociation (x100).
Specifications
Specifications
| Storage Conditions | Stable at room temperature. |
|---|---|
| Specifications | Source: Recombinant Trypsin Solution is produced by submerged microbial fermentation. This solution is animal component-free, and derived from a production process that does not utilize any raw materials and/or processing aids of animal origin. Quality Control: Recombinant Trypsin Solution performance is tested on hMSC. Additional standard evaluations are pH, osmolality, and sterility tests. |
Documentation
Materials Safety Data Sheet
Manuals and Protocols
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
