MSC Attachment Solution

Xeno-free, for attachment and spreading of hMSCs cultured in serum-free conditions

Name SKU Size
MSC Attachment Solution 05-752-1H 5 mL



Product Overview:

The MSC Attachment Solution is a xeno-free, ready-to-use, 100X concentrated solution composed of sterile, affinity purified human fibronectin (hFN) that supports the attachment and spreading of human mesenchymal stem cells (hMSC) cultured in serum-free medium. MSC Attachment Solution application validated and quality tested to provide optimal attachment and growth of hMSCs derived from multiple sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC).  

This attachment solution is also particularly effective for the culture of cells that are not capable of synthesizing their own biomatrix or when culturing cells in serum-free medium. 


  • Xeno-free 
  • Qualified with hMSC from multiple sources
  • Ready-to-use, 100X concentrated solution
  • Designed for use with a serum-free culture system 
  • Suitable for both hMSC proliferation and differentiation assays
mesenchymal stem cells were cultured in MSC NutriStem® XF with and without MSC Attachment Solution

Figure: hMSC-BM cultured in MSC NutriStem® XF on precoated plates with MSC Attachment Solution and without. Representative images were taken at the indicated time points post-seeding (x200).



Form Liquid
Storage Conditions 2-8ºC
Quality Control Each lot is tested for sterility as well as its biological performance (the ability to promote the attachment and spreading of hMSC in serum-free medium).
Specifications The human fibronectin (hFN) used in the MSC Attachment Solution was obtained by affinity purification on gelatine-sepharose from human plasma.
Instructions for Use

Instructions for Use

  1. Dilute MSC Attachment Solution 1:100 with sterile DPBS without Ca2+ and Mg2+ (Cat. No. 02-023-1) and gently pipet to mix.
  2. Coat the culture vessel with the 1:100 diluted MSC Attachment Solution. 
    The volume should be adequate for covering the entire surface of each well or plate. See the table below for recommended coating volumes.
  3. Gently agitate the culture vessel and incubate for a minimum of 30 minutes at 37°C in a 5% CO2 incubator.
  4. Before plating cells, gently remove any remaining liquid MSC Attachment Solution and wash the culture vessel with either 1X DPBS or culture medium.  Do not allow the attachment surface to dry before adding cells.
Culture Vessel Surface area (cm2) 

Volume of 1:100

MSC Attachment Solution

 96 well  0.3  0.1 mL
24 well  1.9  0.4 mL
 12 well  3.8  0.8 mL
 6 well or 35mm dish  9.4  2.0 mL
 T25 Flask   25  5.5 mL
 T75 Flask   75  15 mL


Important Notes 

  1. Coated culture vessels may incubate up to 72 hours with MSC Attachment Solution prior to use.  Incubation up to 72 hours will not decrease cell attachment and cell performance.
  2. Pre-coated culture vessels can be stored sealed with Parafilm® under sterile conditions at 2 to 8°C for up to 1 week prior to use.
  3. Do not allow the MSC Attachment Solution to dry prior to use.



  1. D. Boruczkowski et al. Wharton’s Jelly Mesenchymal Stem Cell Administration Improves Quality of Life and Self-Sufficiency in Children with Cerebral Palsy: Results from a Retrospective Study Stem Cells International, Volume 2019, Article ID 7402151, 13 pages
  2. D.Lisini et al. Adipose tissue-derived mesenchymal stromal cells for clinical application: An efficient isolation approach. Current Research in Translational Medicine,
  3. D. Boruczkowski et al.Third-party Wharton’s jelly mesenchymal stem cells for treatment of steroid-resistant acute and chronic graft-versus-host disease: a report of 10 cases. Turkish Journal of Biology, 40: 493-500, 2016


Materials Safety Data Sheet

Manuals and Protocols

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.
Please enter the product lot number and click search.
If your search does not return any results, please contact us for assistance.


Customer Reviews (1)

Very EfficientReview by Sahar Haimowitz
Great product (Posted on 8/29/2019)

Write Your Own Review

You're reviewing: MSC Attachment Solution

How do you rate this product? *

  1 star 2 stars 3 stars 4 stars 5 stars

You may also be interested in the following product(s)