Description
Details
Product Overview
Please note that PLTGold is available through Biological Industries USA. For bulk orders or more information, contact orders@bioindusa.com.PLTGold® Human Platelet Lysate is a xeno-free, animal serum-free product derived from human platelets. PLTGold® contains all the growth factors and proteins necessary for cell growth, but with reduced turbidity and no clot formation. PLTGold® is the next generation of hPLs which does not require the use of heparin in the manufacturing process.
- Heparin-free and no clot formation
- Effective with MSCs and other primary cells (includes: Mesenchymal Stem/Stromal Cells (graph), Hematopoietic Stem Cells, iPSC and iPSC-derived cells, Embryonic Stem Cells, Fibroblasts, Endothelial Cells, Tumor Cells)
- Cost-effective alternative to FBS (stable supply)
- Provides an optimal environment for cell culture and drug discovery
Why Heparin-Free hPL?
The first platelet lysate, PLTMax®, involves the addition of heparin to the cell culture media to prevent clotting. Heparin is purified and refined according to cGMP methods and is one of the most widely used drugs in humans. There has been no overt requirement for removal of heparin. However, some quality systems have requested its removal or replacement from cell culture processes in an effort to remove all xenogeneic components. PLTGold® is an unfractionated product derived from human platelets that does not require the addition of heparin. It remains clot-free with performance equivalent to the original PLTMax® platelet lysate.
Real time imaging of Adipose-derived MSCs from day 0 to day 5 of culture
Specifications
Specifications
| Origin | Animal serum-free product derived from human platelets |
|---|---|
| Storage Conditions | Store at -20°C for up to 3 years or 4°C for up to 2 weeks Thaw at: 37°C or 4°C |
| Shipping Conditions | Dry Ice |
| Specifications | Using PLTGold®
|
References
references
- Boland L. K., Et al. Nature vs. Nurture: Defining the Effects of Mesenchymal Stromal Cell Isolation and Culture Conditions on Resiliency to Palmitate Challenge. Frontiers in Immunology. 2019.VOL 10.
- Crespo-Diaz R, et al. Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. Cell Transplant. 2011;20(6):797-811.
- Burnouf T, et al. Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation? Biomaterials. 2016 Jan;76:371-87.
Documentation
Manuals & Protocols
Thawing Human MSCs in NutriStem® MSC Medium and Human Platelet Lysate- Passaging MSCs using NutriStem® MSC Medium and Human Platelet Lysate
Product Literature
- Application Note: Growing more cells: cGMP culture environments for high yield expansion of MSCs
- Human Platelet Lysate Product Information Sheet
- PLTGold® Poster presented at ISCT London 2017
- Sample Data: NutriStem® MSC Medium with PLTMax® or PLTGold® Human Platelet Lysate demonstrates superior expansion of MSCs
