Description
Details
Product Overview
Recombinant Trypsin EDTA Solution is a defined, animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues. The EDTA component accelerates the dissociation action of the trypsin enzyme. This solution is optimized for sensitive cells, such as hPSCs and hMSCs.
Recombinant Trypsin EDTA Solution is a pure enzyme solution, which maximizes the yield of functionally viable cells from culture vessels, while preventing toxicity induced by other contaminating proteases. This solution does not contain undesirable proteins such as carboxypeptidase A and chymotrypsin, which are commonly found in porcine or bovine-derived trypsin solutions. In addition, Recombinant Trypsin EDTA Solution eliminates the risk of viruses and other potential adventitious agents that can be carried in animal-derived components.
Features
- Defined, animal component-free
- Ready-to-use solution
- Accelerated enzymatic action
- Contains no contaminating proteases
- Compatible with cells cultured in both serum-free and serum-containing media
Specifications
Specifications
| Storage Conditions | Stable at room temperature. |
|---|---|
| Form | Liquid |
| Specifications | Source: Produced by submerged microbial fermentation. This solution is animal component-free, and derived from a production process that does not utilize any raw materials and/or processing aids of animal origin.
Storage & Stability: Do not store or use past the expiration date Quality Control: Recombinant Trypsin EDTA Solution performance is tested on hMSC. Additional standard evaluations are pH, osmolality, and sterility tests. |
References
references
- Hubel, A. (2018) Cryopreservation of Human Adipose Stem Cells, in Preservation of Cells: A Practical Manual, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118989883.oth4
Documentation
Materials Safety Data Sheet
Manuals and Protocols
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
