BIOGRO-2 SFM, BSA-Free

Serum albumin free, 100 mL
SKU: 05-610-1B

Availability: In stock

$88.00

Description

Details

Product Overview:

BIOGRO-2 formulation is totally free of serum albumin and the concentration of total protein is therefore less than 25 micrograms per ml in the final media.

Applications:

  • Culture of murine myeloma cells (NS-1, NS-0/1, SP2)
  • Culture of mouse-mouse hybridoma cells (from NS-1, NS-0/1, SP2, X63-Aag8-653, BW-S147)
  • Culture of rat-mouse, rat-rat, and human-human hybridomas
  • Monoclonal antibody production
  • Culture of other human, murine and avian lymphocytes
  • Viral production (such as HEV virus from Turkey B-lymphoblastoid cell lines)
  • Culture of various anchorage-dependent cells (such as human osteosarcoma, human epithelial, and thyroid cells)
  • Maintenance of most anchorage-dependent cells during long production periods

  • Method of use:

    BIOGRO-2 is 50 fold concentrates and therefore is recommended for use at a concentration of 2% (although in some cases 1% may be sufficient). DMEM F-12 (1:1) has found to be most generally effective as the basal media, but many cell lines grow well with RPMI 1640, DMEM media or Iscove’s. Glutamine and antibiotics should be added to the final formulation.

Specifications

Specifications

QTY 100 mL
Glucose No Glucose
Storage Conditions -20ºC
Shipping Conditions Dry Ice

References

references

  1. Parnas D, Heldman E, Branski L, et al., Expression and Loaclization of Muscarinic Receptors in P19-Derived Neurons. Journal of Molecular Neuroscience 10: 17-29 (1998)
  2. M Weiler, L Kachko, C Chaimovitz, C Van Kooten and A Douvdevani. CD40 Ligation Enhances IL-15 Production by Tubular Epithelial Cells. Journal of American Society Nephrology 12: 80–87 (2001)
  3. M Joseph Hausmann, B Rogachev, M Weiler, C Chaimovitz and A Douvdevani. Accessory role of human peritoneal mesothelial cells in antigen presentation and T-cell growth. Kidney International 57 :476–486 (2000)
  4. G Kopernik, E Avinoach, Y Grossman,et al., The effect of a high partial pressure of carbon dioxide environment on metabolism and immune functions of human peritoneal cells—Relevance to carbon dioxide pneumoperitoneum. American Journal of Obstetrics & Gynecology 179 (6) :1503-1510 (1998)
  5. M Yanez-Mo, E Lara-Pezzi, R Selgas, et al., Peritoneal Dialysis and Epithelial-to-Mesenchymal Transition of Mesothelial Cells. New England Journal of Medicine 348: 403-413 (2003)
  6. M López-Cabrera, A Aguilera, L S. Aroeira, M Ramírez-Huesca, et al., Ex Vivo Analysis Of Dialysis Effluent-Derived Mesothelial Cells As An Approach To Unveiling The Mechanism Of Peritoneal Membrane Failure. Perit Dial Int 26(1): 26-34 (2006 )
  7. Lewis E, Weiler M, Chaimovitz C, Douvdevani A. Interleukin-15 Is the Main Mediator of Lymphocyte Proliferation in Cultures Mixed With Human Kidney Tubular Epithelial Cells1. Transplantation, 72 (5): 886-890 (2001)
  1. MA Bajo, R Selgas, MA Castro, et al., Low-GDP peritoneal dialysis fluid (‘balance’) has less impact in vitro and ex vivo on epithelial-to-mesenchymal transition (EMT) of mesothelial cells than a standard fluid. Nephrology Dialysis Transplant (2010)
  2. H Eini, N Tejman-Yarden, EC Lewis, et al., Association Between Renal Injury and Reduced Interleukin-15 and Interleukin-15 Receptor Levels in Acute Kidney Injury. Journal of Interferon & Cytokine Research, 30(1): 1-8 (2010)
  3. T Einbinder, Y Sufaro, I Yusim, G Byk, et al., Correction of anemia in uremic mice by genetically modified peritoneal mesothelial cells. Kidney International 63, 2103–2112 (2003)
  4. A Dagan, C Wang, E Fibach, S Gatt. Synthetic, non-natural sphingolipid analogs inhibit the biosynthesis of cellular sphingolipids, elevate ceramide and induce apoptotic cell death. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1633 (3) :161-169 (2003)
  5. B Rogachev, NY Ziv, J Mazar, S Naka, et al., Adenosine is upregulated during peritonitis and is involved in downregulation of inflammation. Kidney International 70: 675–681 (2006)
  6. R Selgas, G del Peso, MA Bajo, et al., Spontaneous VEGF production by cultured peritoneal mesothelial cells from patients on peritoneal dialysis. Peritoneal Dialysis International, 20: 784–801 (2000)

Documentation

Materials Safety Data Sheet

Technical Guide

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