IMDM - Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) with- L-Glutamine, without Alpha-Thioglycerol, without Beta-Mercaptoethanol, is a Dulbecco's modification and is considered a more complex and enriched media. IMDM is utilized for a variety of specialized cell types and also as the basis for some of the more unique serum-free media formulations. The original Iscove's formulation demonstrated that erythrocytes and macrophage precursor cells could be cultured in a totally defined serum-free medium when supplemented with albumin, lecithin, selenium and transferrin. IMDM is a modification of DMEM containing selenium, additional amino acids, vitamins and other components. IMDM is a highly enriched synthetic media well-suited for rapid proliferating and high-density cell cultures in a 5% CO2 atmosphere. Further studies demonstrated that Iscove′s Medium may also support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

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