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Description
Details
Product Overview:
IMDM (Iscove's Modified Dulbecco's Medium) is utilized not only for more specialized cell types but also as the basis for some of the more unique serum-free media formulations. The original formulation designed by Iscove and associates, demonstrated that erythrocytes and macrophage precursor cells (e.g. primary Haemopoietic Cells) could be cultured in a totally defined serum-free medium when supplemented with albumin, lecithin, selenium and transferrin. IMDM is a modification of DMEM (Dulbecco's Modified Eagle's Medium) containing selenium, additional AA's, vitamins and other components. IMDM is a highly enriched synthetic media well-suited for rapidly proliferating and high-density cell cultures in a 5% CO2 atmosphere.
IMDM medium reportedly supports Murine B Lymphocytes, Haemopoietic tissues from Bone Marrow, B-cells stimulated with Lipopolysaccharide, and also T-Lymphocytes and a wide variety of Hybrid cells. When utilized in conjunction with serum, it supports a broad spectrum of Mammalian cell types including but not exclusive to bone marrow, hybridomas and lymphocytes.
This IMDM is modified as follows:
With
- L-Glutamine
- Phenol Red
- Sodium Bicarbonate
- HEPES
Without:
- Beta Mercaptoethanol
- Alpha-Thioglycerol
The complete formulation is available.
Specifications
Specifications
| Glutamine | L-Glutamine |
|---|---|
| QTY | 500 mL |
| Storage Conditions | 2-8°C |
| Form | Liquid |
| HEPES Buffer | HEPES |
| Sodium Bicarbonate Buffer | Sodium Bicarbonate |
| Sodium Pyruvate Additive | with Sodium Pyruvate |
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