Gelatin Solution (0.1%)

Sterile-filtered solution contains 0.1% porcine gelatin in water


Cell-qualified for mouse ES cells

SKU: 01-944-1B

Product Description

Details

Product Overview

Gelatin is a heterogeneous mixture of water-soluble proteins of high-average molecular weights, present in collagen. This sterile-filtered solution contains 0.1% porcine gelatin in water.

Cell-Qualified Gelatin Solution 0.1% is intended for coating cell culture flasks or plates used for the growth of Mouse Embryonic Stem Cells without a feeder layer and with the addition of Leukemia Inhibitory Factor (LIF) to the culture medium.

Additional Information

Additional Information

QTY 100 mL
Form Liquid
Storage Conditions AMB
Shipping Conditions Room Temperature
Instructions for Use
  1. Using aseptic technique, add a sufficient amount of Gelatin Solution to cover the bottom of the culture vessel.  (For example, 2 mL of Gelatin Solution will cover approximately 15 cm2 area.)
  2. Allow the Gelatin Solution to coat the culture vessel at room temperature or in a 37ºC in the incubator for 30 to 60 minutes.
  3. Remove the liquid Gelatin Solution by aspiration just prior to adding culture medium. 

 

Important Notes

  • Do not allow the gelatin solution to evaporate or dry prior to addition of cell culture media.
  • Sealed gelatin-coated plates can be stored for up to one week at room temperature or at 2 to 8ºC.  

Documentation

Materials Safety Data Sheet

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