Description
Details
Product Overview
HEPES is a zwitterionic organic buffer that is also used to maintain physiological pH of cell culture media. HEPES is recommended when the cell culture system is very sensitive, increasing the buffering capacity and stabilizing the pH within the range of 7.2 to 7.6, and is not dependent on CO2 levels.
HEPES is categorized as a "Good" buffer, which derives from a set of buffers described by Dr. Norman Good et al. (Good et. al., Biochemistry 1966).
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES has no nutritional benefit to cells. It is added to the media solely for extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator.
"Good buffers" such as HEPES are attributed with the following characteristics:
- pKa of HEPES is temperature dependent:
At 25ºC: pKa= 7.48
At 37ºC: pKa= 7.31 - High solubility
- Membrane impermeability
- Limited effect on biochemical reactions
- Very low visible and ultraviolet light absorbance
- Chemically and enzymatically stable
- Easy to prepare
Features
- Zwitterionic buffer
- Liquid formulation with high solubility
- Easy-to-use 1M solution
- Ideal for biochemistry, cell culture, and molecular biology applications
Specifications
Specifications
Concentration | 1 M |
---|---|
Storage Conditions | Ambient Temperature |
Shipping Conditions | Room Temperature |
Form | Liquid |
Specifications | Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
The level of HEPES in cell culture media may vary from 10 mM to 25 mM. |
Documentation
Materials Safety Data Sheet
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
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