Waymouth's MB 752/1 Medium

Catalog Number: 01-110-1A

Components

Concentration (mg/L)

INORGANIC SALTS  
Calcium chloride (CaCl2-2H2O) 120.00
Magnesium sulfate (MgSO4-7H20) 200.00
Sodium bicarbonate (NaHCO3) 2240.00
Sodium chloride (NaCl) 6000.00
Potassium phosphate monobasic (KH2PO4) 80.00
Magnesium chloride (MgCl2-6H2O) 240.00
Potassium chloride (KCl) 150.00
Sodium phosphate, dibas (Na2HPO4-7H20) 566.40
OTHER COMPOUNDS  
D-Glucose 5000.00
Glutathione (reduced) 15.00
Hypoxanthine 25.00
Phenol red 10.00
AMINO ACIDS  
L-Arginine hydrochloride 75.00
L-Aspartic acid 60.00
L-Cysteine 61.00
L-Cystine 15.00
L-Glutamic acid 150.00
L-Glutamine 350.00
Glycine 50.00
L-Histidine 128.00
L-Isoleucine 25.00
L-Leucine 50.00
L-Lysine hydrochloride 240.00
L-Methionine 50.00
L-Phenylalanine 50.00
L-Proline 50.00
L-Threonine 75.00
L-Tryptophan 40.00
L-Tyrosine 40.00
L-Valine 65.00
VITAMINS  
Choline chloride 250.00
Vitamin B12 0.20
Folic acid 0.40
Ascorbic acid 17.50
D-Calcium pantothenate 1.00
i-Inositol 0.90
Niacinamide 1.00
Pyridoxine hydrochloride 1.00
Riboflavin 1.00
Biotin 0.02
Thiamine hydrochloride 10.00

Reference:
Waymouth, C. (1968) personal communication.
Ellington, S.K.L. and New, D.A.T. (1980) J. Reprod. Fert., 60:377. (Rat parietal yolk sac)