MCDB 153 (Modified) Medium

Catalog Number: 01-059-1A

Contains L-Glutamine.

Components

Concentration (mg/L)

Ammonium Metavanadate

0.000000585

Calcium Chloride•Anhydrous

0.00333

Cupric Sulfate•5H2O

0.00000275

Ferrous Sulfate•7H2O

0.00139

Magnesium Chloride

0.05713

Molybdic Acid•4H2O (ammonium)

0.00000124

Nickel Chloride•6H2O

0.00000012

Potassium Chloride

0.11183

Sodium Acetate (anhydrous)

0.30153

Sodium Chloride

7.599

Sodium Metasilicate•9H2O

0.000142

Sodium Phosphate Dibasic (anhydrous)

0.284088

Sodium Selenite

0.0000038

Stannous Chloride•2H2O

0.000000113

Zinc Sulfate•7H2O

0.000144

L-Alanine

0.0178

L-Arginine•HCl

0.2107

L-Asparagine•H2O

0.030

L-Aspartic Acid

0.01729

•HCl•H2O

0.04204

L-Glutamic acid

0.0294

L-Glutamine

0.8772

Glycine

0.0150

L-Histidine•HCl•H2O

0.01677

L-Isoleucine

0.001968

L-Leucine

0.0656

L-Lysine•HCl

0.01827

L-Methionine

0.00448

L-Phenylalanine

0.00496

L-Proline

0.04603

L-Serine

0.07356

L-Threonine

0.01191

L-Tryptophan

0.00306

L-Tyrosine•2Na

0.00341

L-Valine

0.03513

D-Biotin

0.0000146

Choline Chloride

0.01396

Folic Acid

0.00079

myo-Inositol

0.01802

Niacinamide

0.00003663

D-Pantothenic Acid (hemicalcium)

0.000238

Pyridoxine•HCl

0.00006171

Riboflavin

0.0000376

Thiamine•HCl

0.000337

Vitamin B-12  0.000407
Adenine•HCl  0.03088
D-Glucose   1.081
HEPES  6.6
Phenol Red•Na   0.001242
Putrescine•2HCl   0.000161
Pyruvic Acid•Na   0.055
Thioctic Acid   0.000206
Thymidine 0.000727
Hydrocortisone   200nM
Triiodothyronine  10nM
Testosteron  10nM
Insulin  5.0mg/litre
Transferrin (Iron-free)   5.0mg/litre
Sodium selenite   5.0microgram/litre