DMEM/F-12, HEPES

Catalog Number: 01-170-1A

This formulation is without L-Glutamine and with Sodium Bicarbonate.

Components

Concentration (mg/L)

INORGANIC SALTS
Calcium chloride (CaCl2) 116.70
Cupric sulfate (CuSO4-5H2O) 0.0013
Ferric nitrate (Fe(NO3)3-9H20) 0.05
Ferrous sulfate (FeSO4-7H2O) 0.417
Potassium chloride (KCl) 311.80
Magnesium chloride (MgCl2) 28.64
Magnesium sulfate (MgSO4) 48.84
Sodium chloride (NaCl) 6995.50
Sodium bicarbonate (NaHCO3) 1200
Sodium phosphate, mono. (NaH2PO4-H20) 62.50
Sodium phosphate, dibas (Na2HPO4) 71.02
Zinc sulfate (ZnSO4-7H2O) 0.432
OTHER COMPOUNDS
D-Glucose 3151.00
Hypoxanthine 2.05
Linoleic Acid 0.042
Lipoic Acid 0.105
Phenol red 8.10
Putrescine-2HCl 0.081
Sodium Pyruvate 55.00
HEPES 3575.00
Thymidine 0.365
AMINO ACIDS
L-Alanine 4.45
L-Arginine hydrochloride 147.50
L-Asparagine-H2O 7.50
L-Aspartic acid 6.65
L-Cysteine-HCl-H2O 17.56
L-Cystine 24.00
L-Glutamic acid 7.35
Glycine 18.75
L-Histidine-HCl-H2O 31.48
L-Isoleucine 54.47
L-Leucine 59.05
L-Lysine hydrochloride 91.25
L-Methionine 17.24
L-Phenylalanine 35.48
L-Proline 17.25
L-Serine 26.25
L-Threonine 53.45
L-Tryptophan 9.02
L-Tyrosine 38.70
L-Valine 52.85
VITAMINS
Biotin 0.0035
D-Calcium pantothenate 2.24
Choline chloride 8.98
Folic acid 2.65
i-Inositol 12.60
Niacinamide 2.02
Pyridoxal hydrochloride 2.00
Pyridoxine hydrochloride 0.031
Riboflavin 0.219
Thiamine hydrochloride 2.17
Vitamin B12 0.68