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DPBS, no calcium, no magnesium

Dulbecco's Phosphate Buffered Saline (DPBS), without Calcium and Magnesium

Bulk packaging options available.

Name SKU Size
DPBS, no calcium, no magnesium 02-023-1A-04 4 x 500 mL
DPBS, no calcium, no magnesium 02-023-1A-12 12 x 500 mL
DPBS, no calcium, no magnesium 02-023-1A-24 24 x 500 mL



Product Overview

Dulbecco's Phosphate Buffered Saline (DPBS) is intended to provide a buffer system for maintaining cell culture media in the physiological range of 7.2– 7.6. It generally serves as an isotonic saline solution or buffer for washing cells and tissues.  It's utility in maintaining mammalian cells when a chemically-defined, balanced salt solution provides the appropriate milieu to sustain the physiological and structural integrity (i.e. tonicity/viability) of cells in vitro for a limited period of time.  Biological Industries offers a range of DPBS formulations. 

DPBS without calcium (Ca2+) and magnesium (Mg2+) is often used in trypsin solutions. DPBS without calcium and magnesium is used in the dissociation process to wash and resuspend cells when the presence of calcium and magnesium may inhibit trypsin activity.

This DPBS is modified as follows:


  • Calcium
  • Magnesium


The complete formulation is available.



Phenol Red Indicator No Phenol Red
Storage Conditions AMB
Sodium Bicarbonate Buffer No Sodium Bicarbonate
Form Liquid
Inorganic Salts No Calcium Chloride, No Magnesium Chloride


Materials Safety Data Sheet

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.

COA's can be downloaded from Sartorius's Certificates Portal.

For certificates issued before November 15, 2021, please enter below the product lot number and click search.
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Customer Reviews (3)

Great productReview by Netta
Good and reliable product. used in our lab for PBMC suspension to prevent cell clumping (Posted on 7/3/2018)
5 starsReview by Liat Amir-Zilberstein
makes life easier... (Posted on 7/3/2018)
A fine productReview by shahar rosenberg
A fine product (Posted on 11/19/2017)

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