Description
Details
Product Overview
NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.
NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.
NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:
- Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)
- Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)
- Human Peripheral Blood Mononuclear Cells (PBMC’s)
- Human Endothelial Cells (EC)
- T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)
- Neuron Cells
- Hybridomas
- CHO cells
- Vero Cells
- Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types
NutriFreez® D10 Cryopreservation Medium Features
- Cryoprotective formulation designed to minimize dehydration effects
- Effective maintenance of cell viability, adhesion, and bioactivity
- Ready-to-use solution, simple protocol
- Suitable for use in cell banks and biopharmaceutical applications
- Animal component-free, serum-free, protein-free solution
- cGMP Manufactured
Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (Nishishita et al. Am J Stem Cells. 2015).
Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez® D10 Cryopreservation Medium exhibit the best post-thaw viability (Salkhordeh et. al. Cytotherapy, May 2018).
Specifications
Specifications
| Storage Conditions | 2-8°C |
|---|---|
| Shipping Conditions | Cold Pack |
| Instructions for Use | Cryopreservation of serum-free cell cultures
Thawing cryopreserved cells
|
References
references
Pluripotent Stem Cells
- Kuznitsov-Yanovsky L., Mayshar Y., Ben-Yosef D. (2019) Modeling FXS: Human Pluripotent Stem Cells and In Vitro Neural Differentiation. In: Ben-Yosef D., Mayshar Y. (eds) Fragile-X Syndrome. Methods in Molecular Biology, vol 1942. Humana Press, New York, NY
- G. Gagliardi et at. Characterization and Transplantation of CD73-Positive Photoreceptors Isolated from Human iPSC-Derived Retinal Organoids. Stem Cell Reports j Vol. 11 j 665–680 j September 11, 2018
- M. Amit, J. Itskovitz-Eldor Single cells pluripotent stem cells in a suspension culture. US Patent App. 16/055,110, 2018
- G. Guo, et al. Epigenetic resetting of human pluripotency." bioRxiv (2017): 146712.
- S. Reichman et al. Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno‐Free and Feeder‐Free Conditions.STEM CELLS 35.5 (2017): 1176-1188.
- F. Pistollato, et al. Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing. JoVE (Journal of Visualized Experiments) 124 (2017): e55702-e55702.
- L. Tian et al. Efficient and controlled generation of 2D and 3D bile duct tissue from human pluripotent stem cell-derived spheroids." Stem Cell Reviews and Reports 12.4 (2016): 500-508.
- J. Chal et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols 11.10 (2016): 1833-1850.
- M. S. Y Hiew. Generation and Microrna Expression Profiling of Colorectal Cancer Cell-Derived Induced Pluripotent Cancer Cells. Diss. UTAR, 2016.
- N. Nishishita et al. An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions. American journal of stem cells 4.1 (2015): 38.
- Z. Danhong, et al. Method of cryopreservation of stem cell-derived retinal pigment epithelial cells on polymeric substrate.U.S. Patent Application No. 14/114,182, 2014
- C. Martin et al. Highly Efficient microRNA-Enhanced mRNA Reprogramming of Diseased Human Fibroblasts in a Feeder-Free Culture System. STEMGENT Application Note, 2014
- C. Buensuceso et al. Induced pluripotent stem cells prepared from human kidney-derived cells . US Patent 20140073049 A1, 2014
- R. Sivapatham, X.Zeng. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods in Molecular Biology, 2014
- L. Tian, N. Prasad, Y. Jang. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2014
- C. Buensuceso et al. Induced pluripotent stem cells from human umbilical cord tissue-derived cells. US Patent 20130157365, 2013
- S. Hikita et al. Methods of Culturing Retinal Pigmented Epithelium Cells, Including Xeno-Free Production, RPE Enrichment, and Cryopreservation. US Patent 20130196369 A1, 2013
- N. Nishishita et al. Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State. PLoS ONE 7(6): e38389. doi:10.1371/journal.pone.0038389 , 2012
- F. Pistollato et al. Standardization of pluripotent stem cell cultures for toxicity testing. Vol. 8, No. 2 , Pages 239-257 (doi:10.1517/17425255.2012.639763), 2012
Mesenchymal Stem Cells
- M. Paz Quesada et al., Safety and Biodistribution of Human Bone Marrow-Derived Mesenchymal Stromal Cells Injected Intrathecally in Non-Obese Diabetic Severe Combined Immunodeficiency Mice: Preclinical Study Tissue Eng Regen Med, 03 July 2019, https://doi.org/10.1007/s13770-019-00202-1
- A.M Lyness et al. Evaluation of a Vial Adaptor to Ensure Safe and Efficient Needle-Free Transfer of Cells Post-Cryopreservation in Preparation for Drug DeliveryCytotherapy, Volume 21, Issue 5, Supplement, May 2019, Page S38
- M. Salkhordeh et al. Evaluation of Different Cryopreservation Agents for Mesenchymal Stem Cell as Final Study Product. Cytotherapy, Volume 20, Issue 5, Supplement, May 2018, Page S50
- Kundrotas, Gabrielis, et al. Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow. Oncotarget 7.10 (2016): 10788.
- Kundrotas, Gabrielis. Investigation of human mesenchymal stem cell genetic characteristics to ensure therapy safety. Diss. Vilnius University, 2016.
Peripheral Blood Mononuclear Cells (PBMC)
- N. Milevoj et. al. A combination of electrochemotherapy, gene electrotransfer of plasmid encoding canine IL-12 and cytoreductive surgery in the treatment of canine oral malignant melanoma. Research in Veterinary Science, volume 122, February 2019, Pages 40-49
Other Cell Lines
- A. C. Brown et al. Long-Term Culture of Nephron Progenitor Cells Ex Vivo. In: Vainio S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY
- E. Nigro, E. Soprana, A. Brini, et l., An Antitumor Cellular Vaccine Based on a Mini-Membrane IgE. J Immunol 188:103-110 (2012).
- G. Di Lullo, E. Soprana, M. Panigada, et al. The combination of marker gene swapping and fluorescence-activated cell sorting improves the efficiency of recombinant modified vaccinia virus Ankara vaccine production for human use. J. of Virological Methods, 163, Issue 2: 195–204 (2010)
- Amit, Michal, and Joseph Itskovitz-Eldor. Novel methods and culture media for culturing pluripotent stem cells. U.S. Patent Application 13/821,244.
- De Falco, Elena, et al. A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts.Cell and tissue banking 14.2 (2013): 277-287.
* The CryoStem™ brand is replaced by the brand NutriFreez®
Documentation
Materials Safety Data Sheet
Manuals and Protocols
Product Literature
NutriFreez® D10 Cryopreservation Medium Product Sheet- NutriFreez® D10 Cryopreservation Medium Performance Validation
Technical Reports
Frequently Asked Questions
*The CryoStem™ brand is replaced by the brand NutriFreez®Certificate of Analysis
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