Y27632 (2HCl)

Highly potent inhibitor of p160-Rho-associated coiled protein kinase (ROCK), 10 mg
SKU: SM-0013-0010

Availability: In stock

$125.00

Description

Details

Product Description

Y27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation and subsequently after FACS analysis or cryopreservation in both feeder-dependent and -independent cultures (Emre, et al.). The enhanced cell attachment and thus recovery is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.).

Product Synonyms:
Y-27632 dihydrochloride, Y-27632, Rock Inhibitor

Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.

Specifications

Specifications

QTY 10 mg
Form Powder
Molecular Weight 320.26
Molecular Formula C14H23Cl2N3O
Chemical Name Cyclohexanecarboxamide, 4-[(1R)-1-aminoethyl]-N-4-pyridinyl-, hydrochloride (1:2), trans
CAS Number 129830-38-2
Target ROCK
Appearance White to off-white (Solid)
Purity ≥95% by HPLC
Solubility and Reconstitution Soluble in DMSO and PBS up to 100 mM, for example:
10 mg/31.225 mL = 0.320 mg/mL = 1 mM
10 mg/6.245 mL = 1.601 mg/mL = 5 mM
10 mg/3.123 mL = 3.202 mg/mL = 10 mM
10 mg/0.624 mL = 16.026 mg/mL = 50 mM
Storage Conditions Store at:
Powder:
-20°C for 3 years
4°C for 2 years

In solvent:
-80°C for 6 months
-20°C for 1 month
Legal This product is for Research Use Only and is not intended for therapeutic or diagnostic use.

References

references

  • Emre, et al. 2010. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. Plos One. 5(8): e12148.
  • Ishizaki, et al. 2000. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Molecular Pharmacology. 57: 976-983.
  • Watanabe, et al. 2007. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nature Biotechnology. 25(6): 681-686.

Documentation

Materials Safety Data Sheet

Technical Data Sheet

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