Description
Details
Product Description
Y27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation and subsequently after FACS analysis or cryopreservation in both feeder-dependent and -independent cultures (Emre, et al.). The enhanced cell attachment and thus recovery is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.).Product Synonyms:
Y-27632 dihydrochloride, Y-27632, Rock InhibitorConstructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.
Specifications
Specifications
| QTY | 10 mg |
|---|---|
| Form | Powder |
| Molecular Weight | 320.26 |
| Molecular Formula | C14H23Cl2N3O |
| Chemical Name | Cyclohexanecarboxamide, 4-[(1R)-1-aminoethyl]-N-4-pyridinyl-, hydrochloride (1:2), trans |
| CAS Number | 129830-38-2 |
| Target | ROCK |
| Appearance | White to off-white (Solid) |
| Purity | ≥95% by HPLC |
| Solubility and Reconstitution | Soluble in DMSO and PBS up to 100 mM, for example: 10 mg/31.225 mL = 0.320 mg/mL = 1 mM 10 mg/6.245 mL = 1.601 mg/mL = 5 mM 10 mg/3.123 mL = 3.202 mg/mL = 10 mM 10 mg/0.624 mL = 16.026 mg/mL = 50 mM |
| Storage Conditions | Store at: Powder: -20°C for 3 years 4°C for 2 years In solvent: -80°C for 6 months -20°C for 1 month |
| Legal | This product is for Research Use Only and is not intended for therapeutic or diagnostic use. |
References
references
- Emre, et al. 2010. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. Plos One. 5(8): e12148.
- Ishizaki, et al. 2000. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Molecular Pharmacology. 57: 976-983.
- Watanabe, et al. 2007. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nature Biotechnology. 25(6): 681-686.
Documentation
Certificate of Analysis
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