Biological Industries is now part of the Sartorius group. Click here for more

Cell Proliferation Kit (XTT based)

One step process, 1000 assays



Will be discontinued from 1/7/2023
SKU: 20-300-1000

Description

Details

Product Overview:

The Cell Proliferation Kit (XTT) was developed for cell proliferation assay in reaction to different growth factors, cytokines and nutrient components. In addition, it is suitable for assaying cytotoxicity of materials such as TNF or other growth inhibitors and for lymphocyte activation. Cell Proliferation Kit (XTT) is an excellent solution for the quantitating of cell proliferation and viability, without using radioactive isotopes. It can be used as a non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity.

Advantages

  • Easy to use, there is no need for additional reagents and/or cell washing procedures.
  • One Step Process, results within 2-5 hours.
  • Sensitivity, cell viability and proliferation can be assayed even in low cell concentrations.
  • Accuracy, dye absorbance is proportional to the number of cells in each well.
  • Safety, there is no need for radioactive isotopes.
  • Convenient, no instrumentation required except for a spectrophotometer (ELISA reader). The entire cell proliferation assay and cell viability assay can be performed directly in a microtiter plate.

Specifications

Specifications

QTY 1000 assays
Storage Conditions -20ºC
Shipping Conditions Dry Ice

References

references

  1. S. Ben-Zichry et al. Synergistic Activity of Anticancer Polyphenols Embedded in Amphiphilic Dendrimer Nanoparticles. ACS Appl. Polym. Mater. 2022
  2. A.G. Mizrahi et al. Loss of Heterozygosity of BRCA1/2 as a Predictive Marker for Talazoparib Response. Anticancer Research, 2022
  3. N. Nevo et al. Thymosin alpha 1 as an adjuvant to hyperthermic intraperitoneal chemotherapy in an experimental model of peritoneal metastases from colonic carcinoma. International Immunopharmacology, Volume 111, October 2022
  4. C. E. Güneş et al. Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells. Gene, 2022
  5. A.S. Pryadko A. et al. Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance. ACS Applied Bio Materials, 2022
  6. A. M. et al. Effects of radiofrequency radiation on apoptotic and antiapoptotic factors in colorectal cancer cells. Biology and Medicine, 2022
  7. Ç. Öner et al. 4-Hydroxycoumarin Effects on Both Cellular and Genetic Characteristics of Hepatocellular Carcinoma Cells. Cytology and Genetics, 2022
  8. N. Wu et al. ZNF582 promoter methylation predicts cervical cancer radiosensitivity and ZNF582 protein overexpression reduces radiosensitivity by cell cycle arrest in S phase. Epigenetics, 2022
  9. A. Hidmi et al. Nitric Oxide-Releasing NO–Curcumin Hybrid Inhibits Colon Cancer Cell Proliferation and Induces Cell Death In Vitro. Processes, 2022
  10. R Strobelt et al. Imatinib inhibits SARS-CoV-2 infection by an off-target-mechanism. Scientific Reports, 2022
  11. E. Güçlü et al. Tomentosin induces apoptosis in pancreatic cancer cells through increasing reactive oxygen species and decreasing mitochondrial membrane potential2022
  12. S. Yalçınkaya et al. Determination of the effect of L. plantarum AB6-25, L. plantarum MK55 and S. boulardii T8-3C microorganisms on colon, cervix, and breast cancer cell lines: Molecular docking, and molecular dynamics study Journal of Molecular Structure, 2022
  13. H. Zhang et al.Anticancer activity of heptazoline against the SCC-15 human oral cancer cells and inhibition of PI3K/AKT signalling pathwayAll Life, 2022
  14. Y.C. Huang et al. Copper-enhanced silver releasing from bimetal-containing bioactive glass (AgCu/80S) elicits antibacterial efficacy against drug-resistant Staphylococcus aureus. Journal of Non-Crystalline Solids, Volume 584, 2022
  15. A. Shai, et al. Inhibiting mutant KRAS G12D gene expression using novel peptide nucleic acid-based antisense: A potential new drug candidate for pancreatic cancer. Oncology Letters, 2022. DOI: 10.3892/ol.2022.13250
  16. R. Esin Ki, et al. Antiproliferative effect of Lactobacillus plantrum L4 strain isolated from Cervicovaginal Microflora on Hela Cancer cell line. Journal of Istanbul Faculty of Medicine, 2022, https://link.gale.com/apps/doc/A691769368/AONE?u=anon~b41d6df5&sid=googleScholar&xid=2a150ffb
  17. E. Thankarajan, et al. A novel, dual action chimera comprising DNA methylating agent and near-IR xanthene-cyanine photosensitizer for combined anticancer therapy. Photodiagnosis and Photodynamic Therapy, 2022, https://doi.org/10.1016/j.pdpdt.2022.102722.
  18. J. Kavaliauskait, et al. Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene. Int. J. Mol. Sci. 2022, https://doi.org/10.3390/ijms23010451
  19. O. Hasin, et al. Theonellamides J and K and 5-cis-Apoa-theopalauamide, Bicyclic Glycopeptides of the Red Sea Sponge Theonella swinhoei. Mar. Drugs 2022 . https://doi.org/10.3390/md20010031
  20. Y.-C. Kuo,et al. Enhanced activity of AZD5582 and SM-164 in rabies virus glycoprotein-lactoferrin-liposomes to downregulate inhibitors of apoptosis proteins in glioblastoma. Materials Science and Engineering: C, 2021, https://doi.org/10.1016/j.msec.2021.112615.
  21. A. Darawsha, et al. The Protective Effect of Carotenoids, Polyphenols, and Estradiol on Dermal Fibroblasts under Oxidative Stress. Antioxidants 2021,  https://doi.org/10.3390/antiox10122023
  22. R. Amara, et al. The Effect of the Classical TSPO Ligand PK 11195 On in Vitro Cobalt Chloride Model of Hypoxia in Non-Neuronal and Brain Cell Lines. Scientific Reports, 2021 DOI: 10.21203/rs.3.rs-1101631/v1
  23. S. Lev-ari, et al. Rh2-enriched Korean ginseng (Ginseng Rh2+) inhibits tumor growth and development of metastasis of non-small cell lung cancer. Food & Function, 2021. DOI: 10.1039/d1fo00643f
  24. E. K. Toruner, et al. The effect of a geometric-shaped tool with blue led light on the activation of human dermal fibroblasts and cancer cells. Journal of Photochemistry and Photobiology, 2021, https://doi.org/10.1016/j.jpap.2021.100087.
  25. R. Miari, et al. 5-aza-2’-deoxycytidine induces apoptosis and inhibits tumour growth in vivo of FaDu cells, a specific HPVnegative HNSCC cell line. PLOS, 2021. https://doi.org/10.1371/journal.pone.0253756
  26. S. Çetinkaya, et al. The Phytochemical Profile and Biological Activity of Liquidambar orientalis Mill. var. orientalis via NF-κB and Apoptotic Pathways in Human Colorectal Cancer. (2021)  Nutrition and Cancer, DOI: 10.1080/01635581.2021.1952455
  27. s. lev-ari, et al. Rh2-enriched Korean ginseng (Ginseng Rh2+) inhibits tumor growth and development of metastasis of non-small cell lung cancer. Food Funct., 2021, https://doi.org/10.1039/D1FO00643F
  28. E.A. Akoulina, et al. Growth of Mesenchymal Stem Cells on Poly(3-Hydroxybutyrate) Scaffolds Loaded with Simvastatin. Bull Exp Biol Med (2021). https://doi.org/10.1007/s10517-021-05190-8
  29. S. Rajendran, et. al. Triphenylphosphonium‐Derived Bright Green Fluorescent Carbon Dots for Mitochondrial Targeting and Rapid Selective Detection of Tetracycline. ChemNanoMat, March 2021 https://doi.org/10.1002/cnma.202100125
  30. A. Bashkin, et al. Forty-One Plant Extracts Screened for Dual Antidiabetic andAntioxidant Functions: Evaluating the Types of Correlationbetweenα-Amylase Inhibition and Free Radical Scavenging. Molecules 2021,26, 317. https://doi.org/10.3390/molecules26020317
  31. M. J. Lee et al. Mutagenicity test of Streptomyces Species culture filtrate using mouse lymphoma cells. IOP Conf. Ser.: Earth Environ. Sci. (2020) 594 012015
  32. J. Cian-Fen, et al. Teaghrelin Protects SH-SY5Y Cells againstMPP+-Induced Neurotoxicity through Activation of AMPK/SIRT1/PGC-1α and ERK1/2 Pathways. Nutrients 2020, 12, 3665; doi:10.3390/nu12123665
  33. S. Palakkal, et al. Tuning the size and hydrophobicity of nanohydrogels exploiting a self-assembly assisted polymerization mechanism for controlled drug delivery. J Nanopart Res 22, 354 (2020). https://doi.org/10.1007/s11051-020-05093-1
  34. E. Hadad, et al. Engineering of NIR fluorescent PEGylated poly(RGD) proteinoid polymers and nanoparticles for drug delivery applications in chicken embryo and mouse models. RSC Adv., 2020, 10, 34364-34372.DOI: 10.1039/D0RA06069K
  35. S. Çetinkaya, et al. Esterification of Fructose-oleic Acid by tert -Butanol/Dimethyl Sulfoxide and by 2-Methyl-2-butanol/Dimethyl Sulfoxide. Journal of Oleo Science, September 10, 2020, doi : 10.5650/jos.ess20142
  36. L.Etta, et al. PEPTIDE KINASE INHIBITORS AND METHODS OF USE THEREOF. US Patent App. N.16/486832, 07/23/2020
  37. C. Chieh Chang, et al. Stimulatory effect of gastroesophageal reflux disease (GERD) on pulmonary fibroblast differentiation. Digestive and Liver Disease, 26 July 2020. https://doi.org/10.1016/j.dld.2020.07.010 
  38. C. Chieh Chang, et al. Regeneration of tooth with allogenous, autoclaved treated dentin matrix with dental pulpal stem cells: An in vivo study. Journal of Endodontics 4 June 2020, https://doi.org/10.1016/j.joen.2020.05.016&nbsp
  39. A. Tombuloglu, et al. In vitro effects of boric acid on human liver hepatoma cell line (HepG2) at the half-maximal inhibitory concentration. Journal of Trace Elements in Medicine and Biology 30 May 2020, 126573, https://doi.org/10.1016/j.jtemb.2020.126573
  40. S. Monga, et al. The protective effect of the TSPO ligands 2,4-Di-Cl-MGV-1, CB86, and CB204 against LPS-induced M1 pro-inflammatory activation of microglia. Brain, Behavior, & Immunity - Health Volume 5, May 2020, https://doi.org/10.1016/j.bbih.2020.100083
  1. P. Botwina et al. Berberine Hampers Influenza A Replication through Inhibition of MAPK/ERK Pathway. Viruses 2020, 12(3), 344; https://doi.org/10.3390/v12030344 
  2. N. Şirin, et al. Investigation of possible effects of apigenin, sorafenib and combined applications on apoptosis and cell cycle in hepatocellular cancer cells. Gene V. 737, 5 May 2020, 144428, https://doi.org/10.1016/j.gene.2020.144428
  3. Y.H. Chang, et al. Human fallopian tube epithelial cells exhibit stemness features, self-renewal capacity, and Wnt-related organoid formation  J Biomed Sci 27, 32 (2020). https://doi.org/10.1186/s12929-019-0602-1
  4. F. Sato, et al. Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis Clocks & Sleep 2020, 2, 26–38; doi:10.3390/clockssleep2010004
  5. R. Sarfstein, et al. Differential Effects of Insulin and IGF1 Receptors on ERK and AKT Subcellular Distribution in Breast Cancer Cells Cells 2019, 8(12), 1499; https://doi.org/10.3390/cells8121499
  6. A. Paul, et al. Tryptophan-Glucosamine Conjugates Modulate Tau-Derived PHF6 aggregation at Low concentrations Chemical Communications, 12 Nov 2019, 10.1039/C9CC06868F
  7. M. Sammar, et al. Correlation between cytotoxicity in cancer cells and free radical‑scavenging activity: In vitro evaluation of 57 medicinal and edible plant extracts Oncology Letters, November 5, 2019 https://doi.org/10.3892/ol.2019.11054 
  8. S.K. Hsieh, et al. Promotion of myotube differentiation and attenuation of muscle atrophy in murine C2C12 myoblast cells treated with teaghrelin  Chemico-Biological Interactions, Volume 315, 5 January 2020, 108893, https://doi.org/10.1016/j.cbi.2019.108893
  9. A. Khattib, et al. Lyso‐diacylglyceryltrimethylhomoserine (lyso‐DGTS) isolated from Nannochloropsis microalgae improves high‐density lipoprotein (HDL) functions International Union of Biochemistry and Molecular Biology, 29 October 2019 https://doi.org/10.1002/biof.1580
  10. N. Reuven et al., Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing Biomolecules 2019, 9, 584; doi:10.3390/biom9100584
  11. Y. C. Kuo, et al. Self-assembled ternary poly(vinyl alcohol)-alginate-gelatin hydrogel with controlled-release nanoparticles for pancreatic differentiation of iPS cells Journal of the Taiwan Institute of Chemical Engineers, 23 September 2019, https://doi.org/10.1016/j.jtice.2019.09.010
  12. S. Yalcin Dextran-coated iron oxide nanoparticle for delivery of miR-29a to breast cancer cell line. Pharmaceutical Development and Technology, volume 24, 2019 - Issue 8
  13. S. Hoffman, et al. Faecal Proteases from Pouchitis Patients Activate Protease Activating Receptor-2 to Disrupt the Epithelial Barrier Journal of Crohn's and Colitis, jjz086, https://doi.org/10.1093/ecco-jcc/jjz086, 06 May 2019
  14. P. Şenel et al. Methyl substituent effect on one‐dimensional copper(II) coordination polymers containing biologically active ligands: Synthesis, characterization, DNA interactions and cytotoxicities Applied Organometallic Chemistry, 24 July 2019, https://doi.org/10.1002/aoc.5122
  15. A. Synowiec et al. Cat flu: Broad spectrum polymeric antivirals Antiviral Research, Volume 170, October 2019, 104563 
  16. L. Achlaug et al. Identification of ZYG11A as a candidate IGF1-dependent proto-oncogene in endometrial cancer. Oncotarget, 2019 Jul 8, 10(43): 4437-4448, 2019 Jul 8, doi: 10.18632/oncotarget.27055
  17. F. Sato et al. Differential expression of claudin‑4, occludin, SOX2 and proliferating cell nuclear antigen between basaloid squamous cell carcinoma and squamous cell carcinoma Molecular Medicine Reports, June 24, 2019 https://doi.org/10.3892/mmr.2019.10417, Pages:1977-1985
  18. Y. Serap, Dextran-coated iron oxide nanoparticle for delivery of miR-29a to breast cancer cell line Pharmaceutical Development and Technology, Pages 1032-1037, 21 Jun 2019
  19. K. C. Huang et al. Hyperglycemia inhibits osteoblastogenesis of rat bone marrow stromal cells via activation of the Notch2 signaling pathway International Journal of Medical Sciences 2019; 16(5): 696-703. doi: 10.7150/ijms.32707
  20. S. Hoffman et al. Faecal Proteases from Pouchitis Patients Activate Protease Activating Receptor-2 to Disrupt the Epithelial Barrier  Journal of Crohn's and Colitis, jjz086, https://doi.org/10.1093/ecco-jcc/jjz086, 06 May 2019
  21. S. Kiraz et al. Antiproliferative activity of copper(II) glutamine complexes with N,N-donor ligands: Synthesis, characterization, potentiometric studies and DNA/BSA interactions. Journal of Molecular Structure, Available online 25 May 2019
  22. S. Monga et al. Inhibitory Effects of the Two Novel TSPO Ligands 2-Cl-MGV-1 and MGV-1 on LPS-induced Microglial Activation. Cells 2019, 8, 486; doi:10.3390/cells8050486
  23. A. Cetin et al. In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines. Czech Journal of Food Sciences, 37, 2019 (1): 0–7
  24. C. C. Chang et al. Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression. International Journal of Medical Sciences, 2019; 16(1): 167-179. doi: 10.7150/ijms.24068
  25. E. Tanış et al. Synthesis, Experimental and Theoretical Analysis, and Antiproliferative Activity of 2-(4-methoxyphenylamino)-2-oxoethyl methacrylate. Chinese Journal of Physics, Available online 22 November 2018
  26. D. İnci et al. Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: Synthesis, X‐ray crystal structures, biomolecular interactions, antioxidant activity, ROS generation and cytotoxicity. Applied Organometallic Chemistry, 2018
  27. S. Izraely et al. The Metastatic Microenvironment: Melanoma‐Microglia Cross‐Talk Promotes the Malignant Phenotype of Melanoma Cells. International Journal of Cancer, 11 July 2018 https://doi.org/10.1002/ijc.31745
  28. A. Szczepanski et al. Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells. Veterinary Research201849:55
  29. Hironori Bando, Yutaka Takahashi et al. Isolated adrenocorticotropic hormone deficiency as a form of paraneoplastic syndrome. Pituitary (2018). https://doi.org/10.1007/s11102-018-0901-7
  30. Y.C. Chen et al. Suppressive effects of metformin on T-helper 1-related chemokines expression in the human monocytic leukemia cell line THP-1. Endocrine Research, 2018
  31. D. İnci et al. Newly synthesized Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes as potential anticancer candidates. Applied Organometallic Chemistry, volume32, Issue4, April 2018
  32. D. Witt-Kehati et al. Inhibition of pMAPK14 Overcomes Resistance to Sorafenib in Hepatoma Cells with Hepatitis B Virus1. Translational Oncology, volume 11, Issue 2, April 2018, pages 511-517
  33. Y.H. Chang et al. Human umbilical cord-derived mesenchymal stem cells reduce monosodium iodoacetate-induced apoptosis in cartilage. Tzu Chi medical Journal, Year : 2018 | Volume : 30 | Issue : 2 | Page : 71-80
  34. V.B. Kumar at al. Ultrafine Highly Magnetic Fluorescent γ-Fe2O3/NCD Nanocomposites for Neuronal Manipulations. ACS Omega 2018 3 (2), 1897-1903
  35. Y.S Chen et al. Characterizing the differentiation of osteoprogenitor cells on surface modified polyether-ether-ketone. Surface and Coatings Technology, 2018
  36. C.W. Chang et al. In Vitro Evaluation of the Antiviral Activity of Some Mushrooms from Turkey. International Journal of Medicinal Mushrooms, issue 3, pages 201-212, 2018
  37. Y.C. Kuo et al. Use of functionalized liposomes loaded with antioxidants to permeate the blood–brain barrier and inhibit β-amyloid-induced neurodegeneration in the brain. Journal of the Taiwan Institute of Chemical Engineers. Volume 87, June 2018, Pages 1-14
  38. M. Bar-Zeev at al. Re-assembled casein micelles for oral delivery of chemotherapeutic combinations to overcome multi drug resistance in gastric cancer. Journal of Molecular and Clinical Medicine, Vol. 1 | Issue 1 | January 2018
  39. G. E. Akbaba et al. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. Marmara Pharmaceutical Journal . 2018, Vol. 22 Issue 2, p299-306. 8p.

Documentation

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.

COA's can be downloaded from Sartorius's Certificates Portal.

For certificates issued before November 15, 2021, please enter below the product lot number and click search.
If your search does not return any results, please contact us for assistance.

Reviews

Customer Reviews (2)

Trustworthy!Review by Raghavendra Basavaraja
Quality
Simplest and most reliable kit for quantifying viable cell numbers. (Posted on 1/27/2018)
ExcellentReview by Orit Sagi-Assif
Quality
The best kit (Posted on 1/9/2018)

Write Your Own Review

You're reviewing: Cell Proliferation Kit (XTT based)

How do you rate this product? *

  1 star 2 stars 3 stars 4 stars 5 stars
Quality

You may also be interested in the following product(s)