Catalog Number: 01-090-1A, 01-090-1B
This formulation is with L-Glutamine
Components |
Concentration (mg/L) |
INORGANIC SALTS |
| Calcium chloride (CaCl2) | 33.30 |
| Cupric sulfate (CuSO4-5H2O) | 0.0025 |
| Ferrous sulfate (FeSO4-7H2O) | 0.834 |
| Potassium chloride (KCl) | 285.00 |
| Potassium phosphate (KH2PO4) | 83.00 |
| Magnesium sulfate (MgSO4) | 74.62 |
| Sodium chloride (NaCl) | 7400.00 |
| Sodium bicarbonate (NaHCO3) | 1200.00 |
| Sodium phosphate, dibas. (Na2HPO4) | 153.70 |
| Zinc sulfate (ZnSO4-7H2O) | 0.0288 |
| OTHER COMPONENTS | |
| D-Glucose | 1100.00 |
| Hypoxanthine Na | 4.70 |
| Lipoic Acid | 0.20 |
| Phenol Red | 1.20 |
| Sodium Pyruvate | 110.00 |
| Thymidine | 0.70 |
| AMINO ACIDS | |
| L-Alanine | 9.00 |
| L-Arginine HCl | 211.00 |
| L-Asparagine H2O | 15.00 |
| L-Aspartic acid | 13.30 |
| L-Cysteine | 25.00 |
| L-Glutamic acid | 14.70 |
| L-Glutamine | 146.00 |
| Glycine | 7.51 |
| L-Histidine HCl H2O | 23.00 |
| L-Isoleucine | 2.60 |
| L-Leucine | 13.00 |
| L-Lysine hydrochloride | 29.00 |
| L-Methionine | 4.48 |
| L-Phenylalanine | 5.00 |
| L-Proline | 11.50 |
| L-Serine | 10.50 |
| L-Threonine | 3.57 |
| L-Tryptophan | 0.60 |
| L-Tyrosine | 1.80 |
| L-Valine | 3.50 |
| VITAMINS | |
| Biotin | 0.024 |
| D-Calcium pantothenate | 0.715 |
| Choline chloride | 0.698 |
| Folic acid | 1.32 |
| i-Inositol | 0.541 |
| Niacinamide | 0.615 |
| Pyridoxine HCl | 0.206 |
| Riboflavin | 0.376 |
| Thiamine hydrochloride | 1.00 |
| Vitamin B12 | 1.36 |
Reference:
Ham, R.G., (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res., 29:515.
