Description
Details
A major step in harvesting cells for chromosome karyotyping is treatment with a hypotonic saline solution to increase cell volume.
Sodium Citrate Solution is a hypotonic solution, which work by creating a concentration gradient across the cytoplasmic membrane and water then rushes in by active transport.
Hypotonic treatment with Sodium Citrate is used most often with the addition of Potassium Chloride (KCl) to enhance membrane permeability and induce hypotonic cell swelling at either room temperature (15-30°C) or 37°C. Usually, the higher temperature is used to increase metaphase spreading.
There are several factors that influence the extent of the hypotonic treatment utilizing Sodium Citrate and/or Potassium Chloride (KCl):
• Cell Density
• Specimen Type (e.g. slides or cell pellet)
Sodium Citrate Solution Features
• Sterile
• Easy-to-use
• Increases metaphase spread of Peripheral Blood Lymphocytes (PBL's)
Specifications
Specifications
| QTY | 500 mL |
|---|---|
| Shipping Conditions | Room Temperature |
| Storage Conditions | AMB |
Documentation
Materials Safety Data Sheet
Certificate of Analysis
Certificates of Analysis (COA's) describe quality control data for specific product lots.
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.
If your search does not return any results, please contact us for assistance.
