Description
Details
Product Description
SANT-1 is a potent antagonist of Smoothened (Smo), which in turn inhibits Hedgehog (Hh) signaling, specifically targeting Shh-LIGHT2 (IC50 of 20nM) and SmoA1-LIGHT2 (IC50 of 30nM). As Smoothened mediates Hedgehog activity, SANT-1 has the potential of affecting cellular patterning through differentiation and proliferation during embryonic development (Chen, et al.). When used in conjunction with CHIR99021 (Cat. No. SM-0001-0010), LDN193189 (Cat. No. SM-0005-0010), and Y27632 (Cat. No. SM-0013-0010), among others, SANT-1 aids in the generation of functional human pancreatic beta cells from human pluripotent stem cells (PSCs) (Pagliuca, et al.) in addition to the creation of pancreatic progenitors from PSCs (Nostro, et al.).Product Synonyms:
Shh signaling antagonist VConstructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.
Specifications
Specifications
| QTY | 10 mg |
|---|---|
| Form | Powder |
| Molecular Weight | 373.49 |
| Molecular Formula | C23H27N5 |
| Chemical Name | N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine |
| CAS Number | 304909-07-7 |
| Target | Smoothened (Smo), Hedgehog (Hh) |
| Appearance | White to off-white (Solid) |
| Purity | ≥95% by LCMS |
| Solubility and Reconstitution | Soluble in DMSO up to 50 mM, for example: 10 mg/26.775 mL = 0.373 mg/mL = 1 mM 10 mg/5.355 mL = 1.867 mg/mL = 5 mM 10 mg/2.677 mL = 3.730 mg/mL = 10 mM 10 mg/1.339 mL = 7.468 mg/mL = 20 mM |
| Storage Conditions | Store at: Powder: -20°C for 3 years 4°C for 2 years In solvent: -80°C for 6 months -20°C for 1 month |
| Legal | This product is for Research Use Only and is not intended for therapeutic or diagnostic use. |
References
references
- Chen, et al. 2002. Small molecule modulation of Smoothened activity. PNAS. 99(22): 14071-14076..
- Nostro, et al. 2015. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Stem Cell Reports. 4(4): 591-604.
- Pagliuca, et al. 2014. Generation of functional human pancreatic β cells in vitro. Cell 159: 428-439.
Documentation
Certificate of Analysis
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