Media 199

M-199 Medium was originally designed for nutritional studies to promote continuous growth of primary chick embryo heart and fibroblast cells without serum supplementation. Biological Industries provides Media 199 with either Earle's or Hank's salts. Media 199 containing Earle's salts (M-199E) are buffered with a bicarbonate/carbonic acid system and will maintain pH in a CO₂incubator. Use of Earle’s salts in atmospheric conditions will result in rapid rise in pH of the culture medium. This medium is also useful with serum for growth of a wide array of cell types including non-transformed human, monkey and chicken cells. Media 199 utilizing Hanks’ salts are buffered with salt solutions designed for atmospheric equilibration and use in a CO₂ incubator will result in a rapid drop in pH of the culture medium.