NutriStem® V9 XF Medium

Defined, xeno-free, serum-free culture medium for hPSC optimized for vitronectin

Name SKU Size Price Qty
NutriStem® V9 XF Medium 05-105-1A-US 500 mL
$195.00

Description

Details

Product Overview

NutriStem® V9 XF Medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human pluripotent stem cells (hPSC), using vitronectin and enzyme-free passage using EDTA. It contains only the essential components required for long-term maintenance of hES and hiPS cells. NutriStem® V9 XF medium shows superior proliferation rates during long-term cultures, while maintaining the pluripotency of the cells.
NutriStem® V9 XF medium allows culture of hPSC in vitronectin pre-coated culture, as well as direct addition of vitronectin to the medium (no need for pre-coating).

Features

  • Produced under cGMP guidelines
  • Maintains human ES and iPS cell pluripotency at high passage
  • Superior results using Vitronectin, may be used with Matrigel coated plates
  • Supports long-term expansion
  • Cytokine-free basal medium, applicable for EB formation, reprogramming, and differentiation
  • Does not contain antibiotics.

Sample Data

Long-term Expansion
Long-term expansion of Hi hESC in NutriStem V9 Vs. E8

Nucleocounts performed on equal volume of cell suspension Chemometech, (count of aggregated cells) during long-term expansion of H1 cultured in NutriStem® V9 XF medium and E8 under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF. NutriStem® V9 XF medium shows superior proliferation rate in long-term culture (over 20 passages)


Cell morphology

Phase contrast images (x100) of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekendfree feeding regime, using 0.5μg/cm2 Vitronectin ACF. Representative images from culture at P6, P16 and P20. Undifferentiated colonies maintained during long-term culture of H1 hESC


Immunofluorescence staining
Tra 1-60
Immunofluorescence analysis of hESC cultured in NutriStem V9 - green stained
Nanog
Immunofluorescence analysis of hESC cultured in NutriStem V9 - red stained
Dapi
Immunofluorescence analysis of hESC cultured in NutriStem V9 - red stained
Merged
Immunofluorescence analysis of hESC cultured in NutriStem V9 - merged

Immunofluorescence analysis of human pluripotent markers of H1 hPSC expanded in NutriStem® V9 XF medium under a weekend-free feeding regime using Vitronectin ACF. Cells from P8 were fixated and stained for the classic pluripotent surface markers: TRA 1-60 (Alexa Fluor) (green) and nuclear conjugated markers: Nanog-RRX, counterstained with DAPI (blue). Scale bar 200μm. Cells cultured in NutriStem® V9 XF Medium on vitronectin express high levels of pluripotent markers


Flow cytometric quantification

Immunophenotyping analysis for human pluripotent markers of H1 hESC culture maintained in NutriStem® V9 XF medium

Immunophenotyping analysis for human pluripotent markers of H1 hESC culture maintained in NutriStem® V9 XF medium under a weekend-free feeding regime using 0.5μg/cm2 Vitronectin ACF at P8 and P17. Data presented as % expression from gated viable cells. High expression of pluripotent stem cell markers in cells cultured in NutriStem® V9 XF medium on vitronectin

Specifications

Specifications

QTY 500 mL
Form Liquid
Brand NutriStem®
Storage Conditions Store at -10° to -20°C
Quality Control Each lot is tested for performance using H1 hESC by expansion of the cells for 3 passages, IF staining for pluripotency marker, and morphology. Other tests include sterility, pH, osmolality and endotoxins levels.
Legal NutriStem® is a registered trademark of Biological Industries​.

Documentation

Certificate of Analysis

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