CryoStem™ MSC Freezing Medium

Chemically defined, animal component-free, protein-free solution

Designed for optimal cryopreservation of hMSCs
Name SKU Size Price Qty
CryoStem™ MSC Freezing Medium 05-712-1E 50 mL



Product Overview:

CryoStem™ MSC Freezing Medium is a chemically defined, animal component-free and protein-free formulation, designed for optimal cryopreservation of human mesenchymal stem cells from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord tissue (UC-MSC).  

When culturing cells under serum free conditions, it is important to cryopreserve the cells also in a solution free of serum.  The novel CryoStem™ MSC Freezing Medium contains methylcellulose and DMSO in place of serum.  After freezing and thawing, a very high percentage of viable hMSCs are obtained with excellent attachment ability as well as growth performance. 

Comparative studies have shown that in most cases higher viabilities and adhesion percentages are obtained in comparison to serum-containing freezing medium. Therefore, the use of this serum-free CryoStem™ MSC Freezing Medium is also recommended for hMSC culture employing serumsupplemented growth media.



  • Chemically defined, animal component-free, protein-free
  • Complete, ready-to-use solution
  • Designed for optimal cryopreservation of hMSCs from a variety of sources
  • High cell attachment and viability after thawing
  • Suitable for cells cultured in either serum-free or serum-containing media
  • Optimized for hMSCs cultuerd with MSC NutriStem® XF Medium
mesenchymal stem cells after cryopreservation using CryoStem™ MSC Freezing Medium

Figure: Recovery of hMSC-BM after thawing procedure. Cells were frozen using CryoStem™ MSC Freezing Medium, thawed and re-seeded in MSC NutriStem® XF on pre-coated plates. Representative images were taken at the indicated time points post-thawing (x200).



Form Liquid
Brand CryoStem™
Storage Conditions 2-8ºC
Quality Control This product is tested for performance using human mesenchymal stem cells (hMSC). Each lot is tested for optimal cell viability, cell attachment, and proliferation after cryopreservation and thawing.



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