Trypan Blue Solution

5mg/ml in Saline | 100 mL
SKU: 03-102-1B

Product Description


Product Overview

Trypan Blue is the stain most commonly used to distinguish viable from nonviable cells. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue. Cells should be in suspension as single cells in buffered saline before counting. Trypan Blue has a higher affinity for serum protein than for cellular proteins, so suspending cells in medium containing serum will generate a dark background.

Instructions for Use

Counting Cells Using The Hemacytometer Method

  • Aseptically withdraw a sample of the cell suspension and prepare 1:2, 1:5, 1:10, or 1:100 dilutions as required in Phosphate Buffered Saline (PBS). Dilute 1:5 in 0.5% Trypan Blue. The optimal concentration of cells for counting is 5-10X105 cells/ml (50-100 cells per large square of the hemocytometer counting chamber) after dilution in the Trypan Blue Solution.
  • After staining with Trypan Blue, the cells should be counted within three (3) minutes; after that interim, the non-viable cells will begin to take up the dye.
  • Using a Pasteur pipette, withdraw a small amount of the stained cell suspension and place the tip of the pipette onto the slot of a clean hemocytometer with a planar coverslip, thereby creating a three-dimensional space. The cell suspension will be transferred under the coverslip by capillary action as the fluid is allowed to flow from the capillary under the coverslip to cover the area of the grid. Next fill the opposite chamber with the second diluted sample. Do not overfill the chamber and do not disturb the coverslip after the hemocytometer has been "charged."
  • Place the hemocytometer on the stage of an inverted microscope using the 10X objective. Adjust focus until a single counting square fills the field. The etched grid marking the boundaries for the counting procedure delineates a specific volume within the space.

Additional Information

Additional Information

QTY 100 mL
Storage Conditions AMB
Shipping Conditions Room Temperature


Materials Safety Data Sheet


Customer Reviews (1)

A fine productReview by shahar rosenberg
A fine product. (Posted on 19/11/2017)

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