Human Pluripotent Stem Cell Culture
Maintaining High-Quality Pluripotent Stem Cells
The transition from stem cell culture research models to clinical applications such as regenerative stem cell therapies require defined, high-quality culture systems and appropriate documentation. Sartorius provides an optimized cell culture environment for human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs), including the NutriStem®, serum-free, xeno-free media family, and its auxiliary reagents, manufactured in accordance with applicable cGMP guidelines.
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NutriStem® hPSC XF Medium
NutriStem® hPSC XF Medium is a powerful low-bFGF medium that works across research and clinical workflows, giving you ultimate control over your cultures.
Defined, xeno-free, serum-free medium
Designed for optimal growth and expansion of human iPSCs and hESCs
Customizable formulation
Custom scale-up services available
Scientific and regulatory support
Drug Master File (DMF) available
Sample media for stem cell-based therapies.
Optimal Culture of hPSCs for Research and Cell Therapies
NutriStem® hPSC XF Medium supports superior growth and expansion of hiPSCs and hESCs.
Xeno-free and serum-free formulation
Performance-validated over long-term culture
Superb cell proliferation
Excellent maintenance of pluripotency, normal karyotype, and gene expression over time
Maximum flexibility — compatible with multiple matrices
Drug Master File (DMF) available
Optimal Medium for Reprogramming and Differentiation
NutriStem® hPSC growth factor (GF)-free contains no TGF or FGF, making it an ideal medium for many applications, such as reprogramming, embryoid body (EB) formation, and various differentiation assays.
Flexible and compatible with multiple applications
Facilitates transition of cells to or from complete NutriStem® hPSC culture medium
Easily modified by the addition of chosen growth factors or cytokines to create a customized complete medium
High Expression of Pluripotent Stem Cell Markers
H1 cells cultured in different media for 6 passages were analyzed using flow cytometry and gene expression. Cells cultured in NutriStem® hPSC XF Medium were >90% positive for SSEA-4 and Oct-4.
Embryoid Body (EB) Formation
hESCs cultured in NutriStem® hPSC XF medium were tested for EBs at 14 days.
The 4 cell types found:
A. Neural rosette
B. Neural rosette stained with tubulin
C. Primitive blood vessels
D. Megakaryocytes
Easy and Reliable Single Cell Passaging
Recovery of H1 (61) hESCs from single-cell passage using NutriStem® hPSC XF and Recombinant Trypsin EDTA Solution on 0.5μg/cm² LaminStem™ 521. Colony morphology shown at 1 day and day 4 post-passage.
Healthy hPSC Colony Morphology
hiPSCs cultured in NutriStem® hPSC XF Medium on Matrigel™ display compact colonies and distinct colony morphology typical of hPSCs.
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Benefits of NutriStem® hPSC XF Media
Achieve superior results and maximum control with the very first defined, xeno-free, serum-free medium for hPSCs.
Maximum flexibility and compatibility — choose the matrix and passaging reagent that best suits your specific applications.
Enhance with additional bFGF for your various culture needs
Easily transfer to the clinic with complete regulatory support
Manufactured in accordance with applicable cGMP guidelines
Drug Master File (DMF) available
Maintain the hPSC full differentiation potential and cell characteristics
Customize for your unique needs — several packaging and volume fill options available including bags for bioprocessing
Enjoy your weekend with a completely weekend-free protocol
FAQs - hPSC Media
Scientists use embryonic stem cells and induced pluripotent stem cells and their derivatives for applications like human development and disease mechanism studies, drug screening, toxicity testing, cell therapy, and organ regeneration. There are several known methods for somatic cells reprograming – the two most common are viral and non-viral RNA- and DNA-based vectors.
Pluripotent stem cells can undergo self-renewal and to differentiate into all cells of the tissues of the body. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) fall under this definition.
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