Description
Details
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
BME contains eight B vitamins, ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.
This BME is modified as follows:
With
- Earle’s Salts Base
- Phenol Red
Without:
- L-Glutamine
- Sodium Bicarbonate
The complete formulation is available.
Specifications
Specifications
Glutamine | No Glutamine |
---|---|
QTY | 500 mL |
Phenol Red Indicator | Phenol Red |
Storage Conditions | 2-8°C |
Form | Liquid |
Sodium Bicarbonate Buffer | no Sodium Bicarbonate |
Documentation
Materials Safety Data Sheet
Certificate of Analysis
COA's can be downloaded from Sartorius's Certificates Portal.
For certificates issued before November 15, 2021, please enter below the product lot number and click search.