EZ-RNA II Total RNA Isolation Kit with BCP, no Chloroform

with BCP instead of Chloroform, 100 mL
SKU: 20-410-100

Availability: In stock

$125.00

Description

Details

Product Overview:

EZ-RNA II Total RNA Isolation Kit is a complete kit with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. The kit contains BCP instead of Chloroform and is an improved version of the Chomczynski and Sacchi method (1),which is based on disruption of cells in guanidine thiocyanate/detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and which allows simultaneous processing of a large number of samples.

1-Bromo-3-chloropropane (BCP) replaces chloroform, a highly volatile and toxic reagent used in molecular biology. Substituting BCP for chloroform in the EZ-RNA II kit, reduces toxic material handling without any adverse effects on the quality of isolated RNA, DNA or proteins. The resulting RNA is suitable for the isolation of Poly A+ RNA or for Northern Blotting, Dot Blotting, in vitro Translation, Molecular Cloning, RT-PCR and RNase Protection Assays, or other analytical procedures. DNA and proteins can also be recovered from the interphase and the organic phase of the same sample.

(1) Chomczynski, P. and Sacchi, N. Anal. Biochem., 162:156-159 (1987)

Specifications

Specifications

QTY 100 mL
Storage Conditions 2-8°C
Shipping Conditions Cold Pack

References

references

  1. A. Bar et al. Live imaging flow bioreactor for the simulation of articular cartilage regeneration after treatment with bioactive hydrogel. Biotechnology and Bioengineering, Volume115, Issue9, September 2018, Pages 2205-2216
  2. N. Torika et al. Angiotensin Converting Enzyme Inhibitors Ameliorate Brain Inflammation Associated with Microglial Activation: Possible Implications for Alzheimer’s Disease. Journal of Neuroimmune Pharmacology, December 2016, Volume 11, Issue 4, pp 774–785
  3. P. Ofek et al. Restoring the oncosuppressor activity of microRNA-34a in glioblastoma using a polyglycerol-based polyplex. Nanomedicine: Nanotechnology, Biology and Medicine, Volume 12, Issue 7, October 2016, Pages 2201-2214
  4. B. C. Cohem et al. Regulation of Lipid Droplet Size in Mammary Epithelial Cells by Remodeling of Membrane Lipid Composition—A Potential Mechanism. PLOS One, 2015, https://doi.org/10.1371/journal.pone.0121645
  5. A. Pinkas et al. An avian model for ascertaining the mechanisms of organophosphate neuroteratogenicity and its therapy with mesenchymal stem cell transplantation. Neurotoxicology and Teratology, Volume 50, July–August 2015, Pages 73-81
  1. E. Markovsky et al. Anticancer polymeric nanomedicine bearing synergistic drug combination is superior to a mixture of individually-conjugated drugs. Journal of Controlled Release, Volume 187, 10 August 2014, Pages 145-157
  2. L. Segal et al. PAR-3 Knockdown Enhances Adhesion Rate of PANC-1 Cells via Increased Expression of Integrinαv and E-Cadherin. PLOS One, 2014, https://doi.org/10.1371/journal.pone.0093879
  3. N. Tatun et al. Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae). European Journal of Entomology . 2014, Vol. 111 Issue 2, p181-188. 8p.

Documentation

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Reviews

Customer Reviews (1)

Very good for RNA isolation from FACS-sorting cellsReview by DOR
Quality
This product, including only three ready-to-use materials, is very suitable for those who seek to extract RNA from low amount of cells. In general, I use it for RNA extraction from FACS-sorting cells. The RNA yield achieved from this kit is sufficient for downstream applications such as qRT-PCR , even if you need to analyze expression of many genes (I do it for 20-30 genes and the results are always reliable and reproducible). The RNA kit protocol, based only on 6 steps, is supposed to be convenient for all users (more and less expert), simplified and clearly written. Moreover, if one of the materials ends (such as the water-saturated phenol, what usually happens to me), you can always buy it separately (e.g. 01-860-1L) and still utilize the other materials, maximizing your money from this useful product. Good Luck! (Posted on 7/11/2018)

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