Description
Details
Product Overview:
EZ-ECL Chemiluminescence detection kit for HRP is a complete kit with ready-to-use reagents for enhanced chemiluminescence detection of immobilized proteins i.e. Western blotting or immobilized nucleic acids i.e. Southern or Northern blotting, conjugated with HRP (Horseradish Peroxidase) directly or indirectly.The use of enhanced chemiluminescence was introduced by Thorpe and Kricka (1,2). In the presence of hydrogen peroxide (H2O2), Horseradish peroxidase (HRP) catalyzes the oxidation of cyclic diacylhydrazides, such as luminol. Immediately following the oxidation, the luminol is in an excited state (intermediate reaction product), which decays to the ground state by emitting light. Strong enhancement of the light emission is produced by enhancers, such as phenolic compounds.
Using this method, it is possible to detect membrane immobilized specific antigens, or sequences of nucleic acids, labeled directly with HRP or indirectly with HRP labeled antibodies/streptavidin.
Advantages
- High sensitivity non-radioactive detection system
- Stable hard copy results on film
- Only small amounts of antibody required
- Detection may be achieved in short exposure times (minutes)
- High resolution
Specifications
Specifications
Storage Conditions | 2-8°C |
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Shipping Conditions | Cold Pack |
References
references
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Documentation
Certificate of Analysis
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Reviews
Customer Reviews (4)
- More than a decade of usage. We just hope one they to buy it in 10L stock....Review by YUVAL
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Quality
(Posted on 7/3/2018) - Cheap, good alternative to popular brandsReview by Tzahi Noiman
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Quality
We submerge blots for three minutes before drying leftover liquids in whatman paper, and then developing in an ECL developer.
Product is cheap, does the same trick as other products, and I would recommend it to those who routinely do WB development and want to save some money. (Posted on 7/3/2018) - Works greatReview by Assaf Ezra
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Quality - We are buying it for years it is good and sensitive Review by YUVAL
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Quality