EZ-ECL Enhanced Chemiluminescence Detection Kit for HRP
Name SKU Size Price Qty
EZ-ECL Kit 20-500-120 120 mL
EZ-ECL Kit 20-500-500 500 mL
EZ-ECL Kit 20-500-1000 1000 mL



Product Overview:

EZ-ECL Chemiluminescence detection kit for HRP is a complete kit with ready-to-use reagents for enhanced chemiluminescence detection of immobilized proteins i.e. Western blotting or immobilized nucleic acids i.e. Southern or Northern blotting, conjugated with HRP (Horseradish Peroxidase) directly or indirectly.

The use of enhanced chemiluminescence was introduced by Thorpe and Kricka (1,2). In the presence of hydrogen peroxide (H2O2), Horseradish peroxidase (HRP) catalyzes the oxidation of cyclic diacylhydrazides, such as luminol. Immediately following the oxidation, the luminol is in an excited state (intermediate reaction product), which decays to the ground state by emitting light. Strong enhancement of the light emission is produced by enhancers, such as phenolic compounds.

Using this method, it is possible to detect membrane immobilized specific antigens, or sequences of nucleic acids, labeled directly with HRP or indirectly with HRP labeled antibodies/streptavidin.


  • High sensitivity non-radioactive detection system
  • Stable hard copy results on film
  • Only small amounts of antibody required
  • Detection may be achieved in short exposure times (minutes)
  • High resolution



Storage Conditions 2-8°C
Shipping Conditions Cold Pack



  1. Z.Yu, et al. Germacrone Induces Apoptosis as Well as Protective Autophagy in Human Prostate Cancer Cells. Cancer Manag Res. 2020;12:4009-4016 https://doi.org/10.2147/CMAR.S250522
  2. H. Xiang,et al. Citrate pretreatment attenuates hypoxia/reoxygenation-induced cardiomyocyte injury via regulating microRNA-142-3p/Rac1 aix. Journal of  Receptors and Signal Transduction,26 May 2020, DOI: 10.1080/10799893.2020.1768548
  3. H. Zhu et al. pSTAT3 Y705 is a prognostic biomarker identified from time-series gene expression profiles of a chemically induced mouse model of hepatocellular carcinoma. Am J Transl Res. 2020; 12(4): 1443–1458. PMID:32355553
  4. J. Zhang et al. Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief Theranostics. 2020 Mar 26; 10(11): 4885–4899. doi: 10.7150/thno.43932
  5. L. Xu, et al. Ginsenoside Rg1-induced activation of astrocytes promotes functional recovery via the PI3K/Akt signaling pathway following spinal cord injury. Life Sciences , 4 April 2020, 117642, https://doi.org/10.1016/j.lfs.2020.117642
  6. R. NAGLER, et al. 18-kDa Translocator Protein Ligands Protect H9C2 Cardiomyocytes from Cigarette Smoke-induced Cell Death: In Vitro Study In Vivo March-April 2020 vol. 34 no. 2 549-556 doi: 10.21873/invivo.11807
  7. G. Fu, et al. CDCA5 functions as a tumor promoter in bladder cancer by dysregulating mitochondria-mediated apoptosis, cell cycle regulation and PI3k/AKT/mTOR pathway activation Journal of Cancer 2020, Vol. 11, doi: 10.7150/jca.35372
  8. M. Villa, et al. Pro-fibrotic effect of oxidized LDL in cardiac myofibroblasts. BBRC, 6 February 2020, https://doi.org/10.1016/j.bbrc.2020.01.156
  9. X. Guo, et al. Macrophage-derived netrin-1 is critical for neuroangiogenesis in endometriosis, International Journal of Biological Macromolecules
    15 January 2020, https://doi.org/10.1016/j.ijbiomac.2020.01.130
  10. I. Olmedo, et al. Inhibition of the proteasome preserves Mitofusin-2 and mitochondrial integrity, protecting cardiomyocytes during ischemia-reperfusion injury (BBA) - Molecular Basis of Disease, 24 December 2019, https://doi.org/10.1016/j.bbadis.2019.165659
  11. Z. Sun, et al. Increased Expression of Gremlin1 Promotes Proliferation and Epithelial Mesenchymal Transition in Gastric Cancer Cells and Correlates With Poor Prognosis of Patients With Gastric Cancer Cancer Genomics and Proteomics January-February 2020 vol. 17 no. 1 49-60 doi: 10.21873/cgp.20167 
  12. C. M.Perelló-Reus, et al. FMS-like tyrosine kinase 3 (FLT3) modulates key enzymes of nucleotide metabolism implicated in cytarabine responsiveness in pediatric acute leukemia Pharmacological Research, 25 November 2019, 104556 https://doi.org/10.1016/j.phrs.2019.104556 
  13. Y. Zhang, et al Knockout of beta‐2 microglobulin reduces stem cell‐induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR‐24/Bim pathway Journal of Cellular and Molecular Medicine, 2019;00:1–16, DOI: 10.1111/jcmm.14778
  14. S. P. Allen, et al C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis Brain, awz302, https://doi.org/10.1093/brain/awz302, 24 October 2019
  15. L. Cui , et al. Ketamine induces endoplasmic reticulum stress in rats and SV-HUC-1 human uroepithelial cells by activating NLRP3/TXNIP aix Biosci Rep (2019) 39 (10), https://doi.org/10.1042/BSR20190595 
  16. R. Xu, et al. CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma Cell Death & Disease volume 10, Article number: 781 (2019)
  17. Y. Wang, et al. Dual-function of Baicalin in nsPEFs-treated Hepatocytes and Hepatocellular Carcinoma cells for Different Death Pathway and Mitochondrial Response Int. J. Med. Sci. 2019, Vol. 16 : 1271-1282. doi: 10.7150/ijms.34876  
  18. Y. Rai, et al. Hexokinase II inhibition by 3-Bromopyruvate sensitizes myeloid leukemic cells K-562 to anti leukemic drug Daunorubicin Institute of Nuclear Medicine and Allied Sciences, Delhi 110 054
  19. F.N. Hamed  et al.  Alopecia areata patients show deficiency of FOXP3 +CD39+ T regulatory cells and clonotypic restriction of Treg TCRβ-chain, which highlights the immunopathological aspect of the disease plos one, July 5, 2019 https://doi.org/10.1371/journal.pone.0210308
  20. B. Liu et al. Recombinant human growth hormone treatment of mice suppresses inflammation and apoptosis caused by skin flap ischemia–reperfusion injury Journal of Cellular Biochemistry, 29 May 2019 https://doi.org/10.1002/jcb.29122
  21. K. Xu et al. LncRNA FOXD2-AS1 plays an oncogenic role in hepatocellular carcinoma through epigenetically silencing CDKN1B(p27) via EZH2 Experimental Cell Research Volume 380, Issue 2, 15 July 2019, Pages 198-204
  22. F. López-Moncada et al. Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer AsianJournal of Andrology, Volume 21 , Issue 4 Page Nos. 319-424
  23. B. Zhu et al. Stem Cell-Derived Exosomes Prevent Aging-Induced Cardiac Dysfunction through a Novel Exosome/lncRNA MALAT1/NF-κB/TNF-α Signaling Pathway Oxidative Medicine and Cellular Longevity, Volume 2019, Article ID 9739258, 14 pages https://doi.org/10.1155/2019/9739258
  24. Y. Lv et al. A Recombinant Measles Virus Vaccine Strain rMV-Hu191 Has Oncolytic Effect against Human Gastric Cancer by Inducing Apoptotic Cell Death Requiring Integrity of Lipid Raft Microdomains. Cancer Letters, 2019
  25. L. Wang et. al. Salmonella spv locus affects type I interferon response and the chemotaxis of neutrophils via suppressing autophagy. Fish & Shellfish Immunology, Volume 87, April 2019, Pages 721-729
  26. J. Wang et al. Proton Pump Inhibitors Inhibit Pancreatic Secretion: Role of Gastric and Non-Gastric H.sup.+/K.sup.+-ATPases. PLoS ONE, vol. 10, no. 5, 2015, p. e0126432. Health Reference Center Academic, Accessed 28 Mar. 2019
  1. Y. Zur et al. A dual-specific macrophage colony-stimulating factor antagonist of c-FMS and [alpha].sub.v[beta].sub.3 integrin for osteoporosis therapy. PLoS Biology, vol. 16, no. 8, 2018, p. e2002979. Health Reference Center Academic, Accessed 28 Mar. 2019
  2. X. Hu et al. Long non-coding RNA BLACAT1 promotes breast cancer cell proliferation and metastasis by miR-150-5p/CCR2. Cell Biosci (2019) 9: 14. https://doi.org/10.1186/s13578-019-0274-2
  3. C. K. Yang et al. The role of glucocorticoids in ovarian development of sleep deprived rats. Taiwanese Journal of Obstetrics and Gynecology, Volume 58, Issue 1, January 2019, Pages 122-127
  4. X. Xu et al. RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism. Thrombosis Research, Volume 176, 2019, Pages 85-94
  5. J. Teng et al. Long non-coding RNA ARAP1-AS1 promotes the progression of bladder cancer by regulating miR-4735-3p/NOTCH2 axis. Cancer Biology & Therapy
  6. A. Konson et al. Pigment Epithelium-Derived Factor and its Phosphomimetic Mutant Induce JNK-Dependent Apoptosis and P38-Mediated Migration Arrest. Cellular Physiology and Biochemistry, 2018
  7. B. Liu et al. Med19 is involved in chemoresistance by mediating autophagy through HMGB1 in breast cancer. Journal of Cellular Biochemistry, volume120, issue 1, 2019, pages 507-518
  8. L. Qin et al. AIP1 suppresses transplant arteriosclerosis through inhibition of vascular smooth muscle cell inflammatory response to IFNγ. The Anatomical Record, 2018
  9. X. Chen et al. Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro. International Journal of Oncology, 2018, Volume 53 Issue 4
  10. M.H. Huang et al. Epigenetic Targeting DNMT1 of Pancreatic Ductal Adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through Hedgehog pathway inhibition. Pharmacological Research, Available online 29 October 2018
  11. X. Huang et al. Ureaplasma spp. lipid-associated membrane proteins induce human monocyte U937 cell cycle arrest through p53-independent p21 pathway. International Journal of Medical Microbiology, Volume 308, Issue 7, October 2018, Pages 819-828
  12. J. Sou et al. Allyl sulfide counteracts 1-bromopropane-induced neurotoxicity by inhibiting neuroinflammation and oxidative stress. Toxicological Sciences, kfy240, https://doi.org/10.1093/toxsci/kfy240
  13. A. Campos et al. Caveolin-1-containing extracellular vesicles transport adhesion proteins and promote malignancy in breast cancer cell lines. Nanomedicine, 2018https://doi.org/10.2217/nnm-2018-0094
  14. E. Bigelman et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLOS One, https://doi.org/10.1371/journal.pone.0198196, 2018
  15. D. Dai et al. Overexpression of farnesyl pyrophosphate synthase increases myocardial ischemia/reperfusion injury in mice. Gene, volume 672, 25 September 2018, Pages 72-78
  16. Zhang J, Liu X. et al. Midazolam Enhances Mutant Huntingtin Protein Accumulation via Impairment of Autophagic Degradation In Vitro. Cell Physiol Biochem 2018;48:683–691
  17. L. Li et al. Psoriasin overexpression confers drug resistance to cisplatin by activating ERK in gastric cancer. International Journal of Oncology, 2018
  18. Lin W. and Chen S. Checkpoint Kinase 1 Is Overexpressed during HPV16-Induced Cervical Carcinogenesis. Gynecologic and Obstetric Investigation, 2018
  19. Y. Yuan et al. Iron Accumulation Leads to Bone Loss by Inducing Mesenchymal Stem Cell Apoptosis Through the Activation of Caspase3. Biological Trace Element Research. https://doi.org/10.1007/s12011-018-1388-9
  20. M. Navarro-Marquez et al. Herpud1 impacts insulin-dependent glucose uptake in skeletal muscle cells by controlling the Ca2+-calcineurin-Akt axis. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Volume 1864, Issue 5, Part A, May 2018, Pages 1653-1662
  21. S.V. Morgan et al. Proteomic and cellular localisation studies suggest non- tight junction cytoplasmic and nuclear roles for occludin in astrocytes. European Journal of Neuroscience, 2018
  22. Z Han, L Shi, Long non-coding RNA LUCAT1 modulates methotrexate resistance in osteosarcoma via miR-200c/ABCB1 axis. Biochemical and Biophysical Research Communications Volume 495, Issue 1, 1 January 2018, Pages 947-953
  23. J. Chen et al. Inhibition of KLHL21 prevents cholangiocarcinoma progression through regulating cell proliferation and motility, arresting cell cycle and reducing Erk activation. Biochemical and Biophysical Research Communications Volume 499, Issue 3, 15 May 2018, Pages 433-440
  24. P. P Lebeau et al. Pharmacologic inhibition of S1P attenuates ATF6 expression, causes ER stress and contributes to apoptotic cell death. Toxicology and Applied Pharmacology, volume 349, 15 June 2018, Pages 1-7
  25. M. Arif et al. Downregulation of aquaporin 3 inhibits cellular proliferation, migration and invasion in the MDA‑MB‑231 breast cancer cell line. Oncolog Letters, July 2018, volume 16 Issue 1
  26. K. Imoto et al. Canstatin modulates L-type calcium channel activity in rat ventricular cardiomyocytes. Biochemical and Biophysical Research Communications, volume 499, Issue 4, 23 May 2018, pages 954-959
  27. D. Lumbroso et al. Macrophage-Derived Protein S Facilitates Apoptotic Polymorphonuclear Cell Clearance by Resolution Phase Macrophages and Supports Their Reprogramming. Frontiers in Immunology, 2018
  28. W. Wu et al. Betaine promotes lipid accumulation in adipogenic-differentiated skeletal muscle cells through ERK/PPARγ signalling pathway. Molecular and Cellular Biochemistry (2018)
  29. J.H. Park et al. EMR, a cytosolic‐abundant ring finger E3 ligase, mediates ER‐associated protein degradation in Arabidopsis. New Phytologist Trust, 2018


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Customer Reviews (4)

More than a decade of usage. We just hope one they to buy it in 10L stock....Review by YUVAL
Tip: Use a 1 mL pipettor for evenly spread A+B mixture on the membrane do avoid waste
(Posted on 7/3/2018)
Cheap, good alternative to popular brandsReview by Tzahi Noiman
I use this product for my western blot development. We switched to it about two years ago, and so far I had no issues with detection, proper development etc.
We submerge blots for three minutes before drying leftover liquids in whatman paper, and then developing in an ECL developer.
Product is cheap, does the same trick as other products, and I would recommend it to those who routinely do WB development and want to save some money. (Posted on 7/3/2018)
Works greatReview by Assaf Ezra
We routinely use this product in our lab. It works great for most applications. Good value for money. (Posted on 11/21/2017)
We are buying it for years it is good and sensitive Review by YUVAL
I would like to recommend this product as it is very sensitive to small proteins and Ab's that cannot be detected by other methods. (Posted on 11/19/2017)

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