EZ-ECL Enhanced Chemiluminescence Detection Kit for HRP
Name SKU Size Price Qty
EZ-ECL Kit 20-500-120 120 mL
EZ-ECL Kit 20-500-500 500 mL
EZ-ECL Kit 20-500-1000 1000 mL



Product Overview:

EZ-ECL Chemiluminescence detection kit for HRP is a complete kit with ready-to-use reagents for enhanced chemiluminescence detection of immobilized proteins i.e. Western blotting or immobilized nucleic acids i.e. Southern or Northern blotting, conjugated with HRP (Horseradish Peroxidase) directly or indirectly.

The use of enhanced chemiluminescence was introduced by Thorpe and Kricka (1,2). In the presence of hydrogen peroxide (H2O2), Horseradish peroxidase (HRP) catalyzes the oxidation of cyclic diacylhydrazides, such as luminol. Immediately following the oxidation, the luminol is in an excited state (intermediate reaction product), which decays to the ground state by emitting light. Strong enhancement of the light emission is produced by enhancers, such as phenolic compounds.

Using this method, it is possible to detect membrane immobilized specific antigens, or sequences of nucleic acids, labeled directly with HRP or indirectly with HRP labeled antibodies/streptavidin.


  • High sensitivity non-radioactive detection system
  • Stable hard copy results on film
  • Only small amounts of antibody required
  • Detection may be achieved in short exposure times (minutes)
  • High resolution



Storage Conditions 2-8°C
Shipping Conditions Cold Pack



  1. S. P. Allen, et al C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis Brain, awz302, https://doi.org/10.1093/brain/awz302, 24 October 2019
  2. L. Cui , et al. Ketamine induces endoplasmic reticulum stress in rats and SV-HUC-1 human uroepithelial cells by activating NLRP3/TXNIP aix Biosci Rep (2019) 39 (10), https://doi.org/10.1042/BSR20190595 
  3. R. Xu, et al. CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma Cell Death & Disease volume 10, Article number: 781 (2019)
  4. Y. Wang, et al. Dual-function of Baicalin in nsPEFs-treated Hepatocytes and Hepatocellular Carcinoma cells for Different Death Pathway and Mitochondrial Response Int. J. Med. Sci. 2019, Vol. 16 : 1271-1282. doi: 10.7150/ijms.34876  
  5. Y. Rai, et al. Hexokinase II inhibition by 3-Bromopyruvate sensitizes myeloid leukemic cells K-562 to anti leukemic drug Daunorubicin Institute of Nuclear Medicine and Allied Sciences, Delhi 110 054
  6. F.N. Hamed  et al.  Alopecia areata patients show deficiency of FOXP3 +CD39+ T regulatory cells and clonotypic restriction of Treg TCRβ-chain, which highlights the immunopathological aspect of the disease plos one, July 5, 2019 https://doi.org/10.1371/journal.pone.0210308
  7. B. Liu et al. Recombinant human growth hormone treatment of mice suppresses inflammation and apoptosis caused by skin flap ischemia–reperfusion injury Journal of Cellular Biochemistry, 29 May 2019 https://doi.org/10.1002/jcb.29122
  8. K. Xu et al. LncRNA FOXD2-AS1 plays an oncogenic role in hepatocellular carcinoma through epigenetically silencing CDKN1B(p27) via EZH2 Experimental Cell Research Volume 380, Issue 2, 15 July 2019, Pages 198-204
  9. F. López-Moncada et al. Secreted protein acidic and rich in cysteine (SPARC) induces epithelial-mesenchymal transition, enhancing migration and invasion, and is associated with high Gleason score in prostate cancer AsianJournal of Andrology, Volume 21 , Issue 4 Page Nos. 319-424
  10. B. Zhu et al. Stem Cell-Derived Exosomes Prevent Aging-Induced Cardiac Dysfunction through a Novel Exosome/lncRNA MALAT1/NF-κB/TNF-α Signaling Pathway Oxidative Medicine and Cellular Longevity, Volume 2019, Article ID 9739258, 14 pages https://doi.org/10.1155/2019/9739258
  11. Y. Lv et al. A Recombinant Measles Virus Vaccine Strain rMV-Hu191 Has Oncolytic Effect against Human Gastric Cancer by Inducing Apoptotic Cell Death Requiring Integrity of Lipid Raft Microdomains. Cancer Letters, 2019
  12. L. Wang et. al. Salmonella spv locus affects type I interferon response and the chemotaxis of neutrophils via suppressing autophagy. Fish & Shellfish Immunology, Volume 87, April 2019, Pages 721-729
  13. J. Wang et al. Proton Pump Inhibitors Inhibit Pancreatic Secretion: Role of Gastric and Non-Gastric H.sup.+/K.sup.+-ATPases. PLoS ONE, vol. 10, no. 5, 2015, p. e0126432. Health Reference Center Academic, Accessed 28 Mar. 2019
  14. Y. Zur et al. A dual-specific macrophage colony-stimulating factor antagonist of c-FMS and [alpha].sub.v[beta].sub.3 integrin for osteoporosis therapy. PLoS Biology, vol. 16, no. 8, 2018, p. e2002979. Health Reference Center Academic, Accessed 28 Mar. 2019
  15. X. Hu et al. Long non-coding RNA BLACAT1 promotes breast cancer cell proliferation and metastasis by miR-150-5p/CCR2. Cell Biosci (2019) 9: 14. https://doi.org/10.1186/s13578-019-0274-2
  16. C. K. Yang et al. The role of glucocorticoids in ovarian development of sleep deprived rats. Taiwanese Journal of Obstetrics and Gynecology, Volume 58, Issue 1, January 2019, Pages 122-127
  17. X. Xu et al. RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism. Thrombosis Research, Volume 176, 2019, Pages 85-94
  18. J. Teng et al. Long non-coding RNA ARAP1-AS1 promotes the progression of bladder cancer by regulating miR-4735-3p/NOTCH2 axis. Cancer Biology & Therapy
  19. A. Konson et al. Pigment Epithelium-Derived Factor and its Phosphomimetic Mutant Induce JNK-Dependent Apoptosis and P38-Mediated Migration Arrest. Cellular Physiology and Biochemistry, 2018
  20. B. Liu et al. Med19 is involved in chemoresistance by mediating autophagy through HMGB1 in breast cancer. Journal of Cellular Biochemistry, volume120, issue 1, 2019, pages 507-518
  21. L. Qin et al. AIP1 suppresses transplant arteriosclerosis through inhibition of vascular smooth muscle cell inflammatory response to IFNγ. The Anatomical Record, 2018
  22. X. Chen et al. Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro. International Journal of Oncology, 2018, Volume 53 Issue 4
  23. M.H. Huang et al. Epigenetic Targeting DNMT1 of Pancreatic Ductal Adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through Hedgehog pathway inhibition. Pharmacological Research, Available online 29 October 2018
  1. X. Huang et al. Ureaplasma spp. lipid-associated membrane proteins induce human monocyte U937 cell cycle arrest through p53-independent p21 pathway. International Journal of Medical Microbiology, Volume 308, Issue 7, October 2018, Pages 819-828
  2. J. Sou et al. Allyl sulfide counteracts 1-bromopropane-induced neurotoxicity by inhibiting neuroinflammation and oxidative stress. Toxicological Sciences, kfy240, https://doi.org/10.1093/toxsci/kfy240
  3. A. Campos et al. Caveolin-1-containing extracellular vesicles transport adhesion proteins and promote malignancy in breast cancer cell lines. Nanomedicine, 2018https://doi.org/10.2217/nnm-2018-0094
  4. E. Bigelman et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLOS One, https://doi.org/10.1371/journal.pone.0198196, 2018
  5. D. Dai et al. Overexpression of farnesyl pyrophosphate synthase increases myocardial ischemia/reperfusion injury in mice. Gene, volume 672, 25 September 2018, Pages 72-78
  6. Zhang J, Liu X. et al. Midazolam Enhances Mutant Huntingtin Protein Accumulation via Impairment of Autophagic Degradation In Vitro. Cell Physiol Biochem 2018;48:683–691
  7. L. Li et al. Psoriasin overexpression confers drug resistance to cisplatin by activating ERK in gastric cancer. International Journal of Oncology, 2018
  8. Lin W. and Chen S. Checkpoint Kinase 1 Is Overexpressed during HPV16-Induced Cervical Carcinogenesis. Gynecologic and Obstetric Investigation, 2018
  9. Y. Yuan et al. Iron Accumulation Leads to Bone Loss by Inducing Mesenchymal Stem Cell Apoptosis Through the Activation of Caspase3. Biological Trace Element Research. https://doi.org/10.1007/s12011-018-1388-9
  10. M. Navarro-Marquez et al. Herpud1 impacts insulin-dependent glucose uptake in skeletal muscle cells by controlling the Ca2+-calcineurin-Akt axis. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Volume 1864, Issue 5, Part A, May 2018, Pages 1653-1662
  11. S.V. Morgan et al. Proteomic and cellular localisation studies suggest non- tight junction cytoplasmic and nuclear roles for occludin in astrocytes. European Journal of Neuroscience, 2018
  12. Z Han, L Shi, Long non-coding RNA LUCAT1 modulates methotrexate resistance in osteosarcoma via miR-200c/ABCB1 axis. Biochemical and Biophysical Research Communications Volume 495, Issue 1, 1 January 2018, Pages 947-953
  13. J. Chen et al. Inhibition of KLHL21 prevents cholangiocarcinoma progression through regulating cell proliferation and motility, arresting cell cycle and reducing Erk activation. Biochemical and Biophysical Research Communications Volume 499, Issue 3, 15 May 2018, Pages 433-440
  14. P. P Lebeau et al. Pharmacologic inhibition of S1P attenuates ATF6 expression, causes ER stress and contributes to apoptotic cell death. Toxicology and Applied Pharmacology, volume 349, 15 June 2018, Pages 1-7
  15. M. Arif et al. Downregulation of aquaporin 3 inhibits cellular proliferation, migration and invasion in the MDA‑MB‑231 breast cancer cell line. Oncolog Letters, July 2018, volume 16 Issue 1
  16. K. Imoto et al. Canstatin modulates L-type calcium channel activity in rat ventricular cardiomyocytes. Biochemical and Biophysical Research Communications, volume 499, Issue 4, 23 May 2018, pages 954-959
  17. D. Lumbroso et al. Macrophage-Derived Protein S Facilitates Apoptotic Polymorphonuclear Cell Clearance by Resolution Phase Macrophages and Supports Their Reprogramming. Frontiers in Immunology, 2018
  18. W. Wu et al. Betaine promotes lipid accumulation in adipogenic-differentiated skeletal muscle cells through ERK/PPARγ signalling pathway. Molecular and Cellular Biochemistry (2018)
  19. J.H. Park et al. EMR, a cytosolic‐abundant ring finger E3 ligase, mediates ER‐associated protein degradation in Arabidopsis. New Phytologist Trust, 2018


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Customer Reviews (4)

More than a decade of usage. We just hope one they to buy it in 10L stock....Review by YUVAL
Tip: Use a 1 mL pipettor for evenly spread A+B mixture on the membrane do avoid waste
(Posted on 7/3/2018)
Cheap, good alternative to popular brandsReview by Tzahi Noiman
I use this product for my western blot development. We switched to it about two years ago, and so far I had no issues with detection, proper development etc.
We submerge blots for three minutes before drying leftover liquids in whatman paper, and then developing in an ECL developer.
Product is cheap, does the same trick as other products, and I would recommend it to those who routinely do WB development and want to save some money. (Posted on 7/3/2018)
Works greatReview by Assaf Ezra
We routinely use this product in our lab. It works great for most applications. Good value for money. (Posted on 11/21/2017)
We are buying it for years it is good and sensitive Review by YUVAL
I would like to recommend this product as it is very sensitive to small proteins and Ab's that cannot be detected by other methods. (Posted on 11/19/2017)

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