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DPBS (10X), no calcium, no magnesium

Dulbecco's Phosphate Buffered Saline (DPBS)

10X Solution, Without Calcium and Magnesium
Name SKU Size
DPBS (10X), no calcium, no magnesium 02-023-5A-04 4 x 500 mL
DPBS (10X), no calcium, no magnesium 02-023-5A-12 12 x 500 mL
DPBS (10X), no calcium, no magnesium 02-023-5A-24 24 x 500 mL



Product Overview

Dulbecco's Phosphate Buffered Saline (DPBS) is intended to provide a buffer system for maintaining cell culture media in the physiological range of 7.2– 7.6. It generally serves as an isotonic saline solution or buffer for washing cells and tissues.  It's utility in maintaining mammalian cells when a chemically-defined, balanced salt solution provides the appropriate milieu to sustain the physiological and structural integrity (i.e. tonicity/viability) of cells in vitro for a limited period of time.  Biological Industries offers a range of DPBS formulations. 

DPBS without calcium (Ca2+) and magnesium (Mg2+) is often used in trypsin solutions. DPBS without calcium and magnesium is used in the dissociation process to wash and resuspend cells when the presence of calcium and magnesium may inhibit trypsin activity.

This DPBS is a 10X solution, modified as follows:


  • Calcium
  • Magnesium


The complete formulation is available.



QTY 24 x 500 mL
Phenol Red Indicator No Phenol Red
Concentration 10X
Storage Conditions AMB
Shipping Conditions Room Temperature
Sodium Bicarbonate Buffer No Sodium Bicarbonate
Form Liquid
Inorganic Salts No Calcium, No Magnesium


Materials Safety Data Sheet

Certificate of Analysis

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