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Low protein serum-free medium, designed for hybridoma cell growth and monoclonal antibody production
SKU: 05-015-1



Product Overview:

DCCM-2 is serum-free medium, designed for hybridoma cell growth and monoclonal antibody production. The medium is a low protein formulation, and it is ready-to-use and needs only the addition of L-Glutamine. DCCM-2 contains BSA.


  • Culture of myeloma and hybridoma cells
  • Monoclonal antibody production
  • Culture of human lymphocyte cells (including stimulated or transformed cells)
  • Viral production



QTY 500 mL
Glutamine No Glutamine
Storage Conditions 2-8°C



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  9. S. Hinderlich et al. The homozygous M712T mutation of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase results in reduced enzyme activities but not in altered overall cellular sialylation in hereditary inclusion body myopathy. FEBS Letters, Volume 566, Issue 1-3, May 21, 2004, Pages 105–109
  1. Mariani E, Mariani AR, Monaco MC, et al., Commercial serum-free media: hybridoma growth and monoclonal antibody production. Journal of Immunological Methods, 145 (1-2): 175-183 (1991).
  2. Graf H, Rabaud JN, Egly JM. Screening of various mammalian cell culture media to establish a downstream purification scheme. Journal of Immunological Methods, 139(1): 135-144 (1991).
  3. Fernández E, Fallon MJ, Frazier ML, et al., Expression of acinar and ductal products in capan-1 cells growing in synthetic serum and serum-free media Cancer 73(9): 2285-2295 (1994).
  4. Aparna Raval, Niti Ipuri and R. K. Saxena. Generation of human class I major histocompatibility complex activating factor in serum free medium and its partial characterization. Journal of Biosciences 22: 59-68 (1997).
  5. JR de los Toyos, FJ Mendez, JF Aparicio, et al., Functional analysis of pneumolysin by use of monoclonal antibodies. Infect. Immun. 64 (2): 480-484 (1996).


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