DCCM-2 SFM

Low protein serum-free medium, designed for hybridoma cell growth and monoclonal antibody production
SKU: 05-015-1

Availability: In stock

$75.00

Description

Details

Product Overview:

DCCM-2 is serum-free medium, designed for hybridoma cell growth and monoclonal antibody production. The medium is a low protein formulation, and it is ready-to-use and needs only the addition of L-Glutamine. DCCM-2 contains BSA.

Applications

  • Culture of myeloma and hybridoma cells
  • Monoclonal antibody production
  • Culture of human lymphocyte cells (including stimulated or transformed cells)
  • Viral production

Specifications

Specifications

QTY 500 mL
Glutamine No Glutamine
Storage Conditions 2-8°C

References

references

  1. I.W.H. Jarvis et al. Genotoxicity of fine and coarse fraction ambient particulate matter in immortalised normal (TT1) and cancer-derived (A549) alveolar epithelial cells. Environ. Mol. Mutagen. 2018
  2. P. Chanana et al. Mutation in GNE Downregulates Peroxiredoxin IV Altering ER Redox Homeostasis. NeuroMolecular Medicine, December 2017, Volume 19, Issue 4, pp 525–540
  3. F. Conforti et al. The histone deacetylase inhibitor, romidepsin, as a potential treatment for pulmonary fibrosis. Oncotarget. 2017 Jul 25; 8(30): 48737–48754
  4. R. Singh, R. Arya, GNE myopathy and cell apoptosis: A comparative mutation analysis. Molecular Neurobiology, (2016) 53: 3088
  5. M. Eisenbach-Schwartz et al. Human monocyte sub-population for treatment of central nervous system injury. US Patent, US9314483B2, 2016
  6. M. Grossman et al. Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril Alignment That Control Assembly of the Extracellular Matrix. Cancer Research, July 2016, Volume 76, Issue 14
  7. J.R. Cooper et al. Long Term Culture of the A549 Cancer Cell Line Promotes Multilamellar Body Formation and Differentiation towards an Alveolar Type II Pneumocyte Phenotype. PloS one, October 28, 2016
  8. Y. Udi et al. Inhibition Mechanism of Membrane Metalloprotease by an Exosite-Swiveling Conformational Antibody. Structure, volume 23, Issue 1, 6 January 2015, Pages 104-115
  9. S. Hinderlich et al. The homozygous M712T mutation of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase results in reduced enzyme activities but not in altered overall cellular sialylation in hereditary inclusion body myopathy. FEBS Letters, Volume 566, Issue 1-3, May 21, 2004, Pages 105–109
  1. Mariani E, Mariani AR, Monaco MC, et al., Commercial serum-free media: hybridoma growth and monoclonal antibody production. Journal of Immunological Methods, 145 (1-2): 175-183 (1991).
  2. Graf H, Rabaud JN, Egly JM. Screening of various mammalian cell culture media to establish a downstream purification scheme. Journal of Immunological Methods, 139(1): 135-144 (1991).
  3. Fernández E, Fallon MJ, Frazier ML, et al., Expression of acinar and ductal products in capan-1 cells growing in synthetic serum and serum-free media Cancer 73(9): 2285-2295 (1994).
  4. Aparna Raval, Niti Ipuri and R. K. Saxena. Generation of human class I major histocompatibility complex activating factor in serum free medium and its partial characterization. Journal of Biosciences 22: 59-68 (1997).
  5. JR de los Toyos, FJ Mendez, JF Aparicio, et al., Functional analysis of pneumolysin by use of monoclonal antibodies. Infect. Immun. 64 (2): 480-484 (1996).

Documentation

Materials Safety Data Sheet

Technical Guide

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