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DCCM-1 SFM

High protein serum-free medium, designed for hybridoma cell growth and monoclonal antibody production
SKU: 05-010-1A

Availability: In stock

$58.00

Description

Details

Product Overview:

DCCM-1 is serum-free medium, designed for hybridoma cell growth and monoclonal antibody production. The medium is a high protein formulation for maximizing cell growth, and it is ready-to-use and needs only the addition of L-Glutamine. DCCM-1 contains BSA.

Applications

  • Culture of myeloma and hybridoma cells
  • Monoclonal antibody production
  • Culture of human lymphocyte cells (including stimulated or transformed cells)

Specifications

Specifications

QTY 500 mL
Glutamine No Glutamine
Storage Conditions 2-8°C
Shipping Conditions Cold Pack

References

references

  1. I.W.H. Jarvis et al. Genotoxicity of fine and coarse fraction ambient particulate matter in immortalised normal (TT1) and cancer-derived (A549) alveolar epithelial cells. Environ. Mol. Mutagen. 2018
  2. P. Chanana et al. Mutation in GNE Downregulates Peroxiredoxin IV Altering ER Redox Homeostasis. NeuroMolecular Medicine, December 2017, Volume 19, Issue 4, pp 525–540
  3. F. Conforti et al. The histone deacetylase inhibitor, romidepsin, as a potential treatment for pulmonary fibrosis. Oncotarget. 2017 Jul 25; 8(30): 48737–48754
  4. R. Singh, R. Arya, GNE myopathy and cell apoptosis: A comparative mutation analysis. Molecular Neurobiology, (2016) 53: 3088
  5. M. Eisenbach-Schwartz et al. Human monocyte sub-population for treatment of central nervous system injury. US Patent, US9314483B2, 2016
  6. M. Grossman et al. Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril Alignment That Control Assembly of the Extracellular Matrix. Cancer Research, July 2016, Volume 76, Issue 14
  7. J.R. Cooper et al. Long Term Culture of the A549 Cancer Cell Line Promotes Multilamellar Body Formation and Differentiation towards an Alveolar Type II Pneumocyte Phenotype. PloS one, October 28, 2016
  8. Y. Udi et al. Inhibition Mechanism of Membrane Metalloprotease by an Exosite-Swiveling Conformational Antibody. Structure, volume 23, Issue 1, 6 January 2015, Pages 104-115
  9. S. Hinderlich et al. The homozygous M712T mutation of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase results in reduced enzyme activities but not in altered overall cellular sialylation in hereditary inclusion body myopathy. FEBS Letters, Volume 566, Issue 1-3, May 21, 2004, Pages 105–109
  10. McClelland AR, Barrow D, Madden TA, et al., Enhanced Epidermal Growth Factor Receptor Signaling in MCF7 Breast Cancer Cell after Long-Term Culture in the presence of the Pure Antiestrogen ICI 182,780 (Faslodex). Endocrinology 142 (7): 2776-2788 (2001)
  11. Suarez Y, Fernandez C, Ledo B, et al., Differential effects of ergosterol and cholesterol on Cdk1 activation and SRE-driven transcription. Eur. J. Biochem. 269 (6): 1761-1771 (2002)
  1. Teitelbaum D, Arnon R, Sela M, et al., Immunomodulation of experimental autoimunne encephalomyelitis by oral administration of copolymer 1. Proc. Natl. Acad. Sci. USA 96 (7): 3842-3847 (1999).
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  3. Fares FA, Levi F, Reznick AZ, Kraiem Z. Engineering a Potential Antagonist of Human Thyrotropin and Thyroid-stimulating Antibody. J. Biol. Chem. 276 (7): 4543-4548 (2001).
  4. Kraiem Z, Sadeh O, Yosef M. Iodide uptake and origanification, tri-iodothyronine secretion, cyclic AMP accumulation and cell proliferation in an optimized system of human thyroid follicles cultured in collagen gel suspended in serum-free medium. Journal of Endocrinology 131(3): 499-506 (1991).
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  6. Heinrich R, Kraiem Z. The Protein Kinase A Pathway Inhibits c-jun and c-fos Protooncogene Expression Induced by the Protein Kinase C and Tyrosine Kinase Pathways in Cultured Human Thyroid Follicles. Journal of Clinical Endocrinology and Metabolism 82 (6): 1839-1844 (1997).
  7. Leshem B, Brass D. Mouse lymphoblasts lose their immunogenicity and susceptibility to specific cytotoxic T lymphocyte lysis during maintenance in culture. Immunology 95(3): 409-418 (1998).
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Documentation

Materials Safety Data Sheet

Technical Guide

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