CryoStem™ hPSC Freezing Medium

Chemically defined, animal component-free, protein-free cryopreservation solution for human ES cells and iPS cells.
Name SKU Size Price Qty
CryoStem™ hPSC Freezing Medium 05-710-1E 50 mL
$90.00

Description

Details

Product Overview

CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem™ hPSC Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem™ hPSC Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem™ hPSC Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.

CryoStem™ hPSC Freezing Medium contains methylcellulose and DMSO in place of serum.  The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.  

CryoStem™ hPSC Freezing Medium Features

  • Chemically defined, Animal component-free (ACF), Protein-free
  • Works with various media
  • Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
  • High recovery efficiency: maintains excellent attachment ability as well as growth performance
  • Maintains hESC and hiPSC pluripotency
  • Complete formulation; Ready-to-use at 2-8°C
Alkaline Phosphatase staining of emnryonic stem cells recovering from freezing in cryostem

Alkaline Phosphatase (AP) staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem™ hPSC Freezing Medium. Cells were maintained on feeder layer. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.

AP staining of embryonic stem cells after recovering from freezing in CroStem freezing solution

AP staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem™ hPSC Freezing Medium. Cells were maintained in feeder-free conditions. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.

SSEA-4 at Passage 2

H1 cell morphology and immunofluorescence analysis of hESC marker SSEA-4 at Passage 2, recovered from freezing condition in CryoStem™ hPSC Freezing Medium.

H1 hESC BGO1V/hOG cels (Variant hESC hOct4-GFP reporter cells)

1 hour post-thawing

Day 1 Post-thawing

Day 4 Post-thawing

hESC were frozen in CryoStem™ hPSC Freezing Medium and were thawed into NutriStem® hESC XF on Matrigel.

Specifications

Specifications

Form Liquid
Brand CryoStem™
Storage Conditions Store at 2 - 8ºC​
Instructions for Use Do not use if a visible precipitate is observed in the medium. Do not use beyond the expiration date indicated on the product label.
Legal CryoStem™ hPSC Freezing Medium is intended for in vitro diagnostic use

References

references

Documentation

Certificate of Analysis

Certificates of Analysis (COA's) describe quality control data for specific product lots.
Please enter the product lot number and click search.
If your search does not return any results, please contact us for assistance.

Reviews

Customer Reviews (4)

Good product for cells growing without serumReview by Shaked Eliyahu
Quality
I use this product regularly with cells which are not supposed to receive serum. It is very good and the cells recover quickly. I really recommend this product. (Posted on 7/3/2018)
This is a recommendable product.Review by Zhiyuan Lv
Quality
This is my first use, and it will be my only choice. It is convenient to use and increases of cell viabilities. (Posted on 2/5/2018)
great productReview by Amira gepstein
Quality
I freeze IPS cells with great succses. Its easier and more effective then freezing with

FBS(20%+ DMSO(10%or 20%). (Posted on 1/10/2018)
Great product for stem cell long term freezing!Review by Bar Philosof
Quality
I use it to freeze cancer stem-like cell, and it is a great product. (Posted on 11/20/2017)

Write Your Own Review

You're reviewing: CryoStem™ hPSC Freezing Medium

How do you rate this product? *

  1 star 2 stars 3 stars 4 stars 5 stars
Quality

You may also be interested in the following product(s)