CryoStem™ hPSC Freezing Medium

Chemically defined, animal component-free, protein-free cryopreservation solution for human ES cells and iPS cells.
Name SKU Size Price Qty
CryoStem™ hPSC Freezing Medium 05-710-1D 10 mL
$30.00
CryoStem™ hPSC Freezing Medium 05-710-1E 50 mL
$90.00

Description

Details

Product Overview

CryoStem™ hPSC Freezing Medium is a ready-to-use solution for the serum-free, animal component-free cryopreservation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Cells preserved with CryoStem™ hPSC Freezing Medium show high viability and recovery after thawing, and express typical pluripotent markers in culture. CryoStem™ hPSC Freezing Medium maintains xeno-free conditions during cryopreservation when culturing cells in a xeno-free environment. CryoStem™ hPSC Freezing Medium has been tested on human ES cells (H1, H9 and HuES9). Superior results were obtained in comparison with both serum-containing freezing media as well as competing serum-free products.

CryoStem™ hPSC Freezing Medium contains methylcellulose and DMSO in place of serum.  The culture of cells in animal component-free medium eliminates the risks associated with animal sera and serum-derived products.  

CryoStem™ hPSC Freezing Medium Features

  • Chemically defined, Animal component-free (ACF), Protein-free
  • Works with various media
  • Suitable for freezing hESC and hiPSC cultured in both feeder and feeder-free conditions
  • High recovery efficiency: maintains excellent attachment ability as well as growth performance
  • Maintains hESC and hiPSC pluripotency
  • Complete formulation; Ready-to-use at 2-8°C
Alkaline Phosphatase staining of emnryonic stem cells recovering from freezing in cryostem

Alkaline Phosphatase (AP) staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem™ hPSC Freezing Medium. Cells were maintained on feeder layer. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.

AP staining of embryonic stem cells after recovering from freezing in CroStem freezing solution

AP staining of H1 cells at Passage 2 recovered from freezing condition in CryoStem™ hPSC Freezing Medium. Cells were maintained in feeder-free conditions. Cell colony exhibits a distinct morphology typical of pluripotent hESCs.

SSEA-4 at Passage 2

H1 cell morphology and immunofluorescence analysis of hESC marker SSEA-4 at Passage 2, recovered from freezing condition in CryoStem™ hPSC Freezing Medium.

H1 hESC BGO1V/hOG cels (Variant hESC hOct4-GFP reporter cells)

1 hour post-thawing

Day 1 Post-thawing

Day 4 Post-thawing

hESC were frozen in CryoStem™ hPSC Freezing Medium and were thawed into NutriStem® hESC XF on Matrigel.

Specifications

Specifications

Form Liquid
Brand CryoStem™
Storage Conditions Store at 2 - 8ºC​
Instructions for Use Do not use if a visible precipitate is observed in the medium. Do not use beyond the expiration date indicated on the product label.
Legal CryoStem™ hPSC Freezing Medium is intended for in vitro diagnostic use

References

references

Documentation

Reviews

Customer Reviews (3)

This is a recommendable product.Review by Zhiyuan Lv
Quality
This is my first use, and it will be my only choice. It is convenient to use and increases of cell viabilities. (Posted on 2/5/2018)
great productReview by Amira gepstein
Quality
I freeze IPS cells with great succses. Its easier and more effective then freezing with

FBS(20%+ DMSO(10%or 20%). (Posted on 1/10/2018)
Great product for stem cell long term freezing!Review by Bar Philosof
Quality
I use it to freeze cancer stem-like cell, and it is a great product. (Posted on 11/20/2017)

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