Cell Proliferation Kit (XTT based)

One step process, 1000 assays




SKU: 20-300-1000

Description

Details

Product Overview:

The Cell Proliferation Kit (XTT) was developed for cell proliferation assay in reaction to different growth factors, cytokines and nutrient components. In addition, it is suitable for assaying cytotoxicity of materials such as TNF or other growth inhibitors and for lymphocyte activation. Cell Proliferation Kit (XTT) is an excellent solution for the quantitating of cell proliferation and viability, without using radioactive isotopes. It can be used as a non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity.

Advantages

  • Easy to use, there is no need for additional reagents and/or cell washing procedures.
  • One Step Process, results within 2-5 hours.
  • Sensitivity, cell viability and proliferation can be assayed even in low cell concentrations.
  • Accuracy, dye absorbance is proportional to the number of cells in each well.
  • Safety, there is no need for radioactive isotopes.
  • Convenient, no instrumentation required except for a spectrophotometer (ELISA reader). The entire cell proliferation assay and cell viability assay can be performed directly in a microtiter plate.

Specifications

Specifications

QTY 1000 assays
Storage Conditions -20ºC
Shipping Conditions Dry Ice

References

references

  1. E. Hadad, et al. Engineering of NIR fluorescent PEGylated poly(RGD) proteinoid polymers and nanoparticles for drug delivery applications in chicken embryo and mouse models. RSC Adv., 2020, 10, 34364-34372.DOI: 10.1039/D0RA06069K
  2. S. Çetinkaya, et al. Esterification of Fructose-oleic Acid by tert -Butanol/Dimethyl Sulfoxide and by 2-Methyl-2-butanol/Dimethyl Sulfoxide. Journal of Oleo Science, September 10, 2020, doi : 10.5650/jos.ess20142
  3. L.Etta, et al. PEPTIDE KINASE INHIBITORS AND METHODS OF USE THEREOF. US Patent App. N.16/486832, 07/23/2020
  4. C. Chieh Chang, et al. Stimulatory effect of gastroesophageal reflux disease (GERD) on pulmonary fibroblast differentiation. Digestive and Liver Disease, 26 July 2020. https://doi.org/10.1016/j.dld.2020.07.010 
  5. C. Chieh Chang, et al. Regeneration of tooth with allogenous, autoclaved treated dentin matrix with dental pulpal stem cells: An in vivo study. Journal of Endodontics 4 June 2020, https://doi.org/10.1016/j.joen.2020.05.016&nbsp
  6. A. Tombuloglu, et al. In vitro effects of boric acid on human liver hepatoma cell line (HepG2) at the half-maximal inhibitory concentration. Journal of Trace Elements in Medicine and Biology 30 May 2020, 126573, https://doi.org/10.1016/j.jtemb.2020.126573
  7. S. Monga, et al. The protective effect of the TSPO ligands 2,4-Di-Cl-MGV-1, CB86, and CB204 against LPS-induced M1 pro-inflammatory activation of microglia. Brain, Behavior, & Immunity - Health Volume 5, May 2020, https://doi.org/10.1016/j.bbih.2020.100083
  8. P. Botwina et al. Berberine Hampers Influenza A Replication through Inhibition of MAPK/ERK Pathway. Viruses 2020, 12(3), 344; https://doi.org/10.3390/v12030344 
  9. N. Şirin, et al. Investigation of possible effects of apigenin, sorafenib and combined applications on apoptosis and cell cycle in hepatocellular cancer cells. Gene V. 737, 5 May 2020, 144428, https://doi.org/10.1016/j.gene.2020.144428
  10. Y.H. Chang, et al. Human fallopian tube epithelial cells exhibit stemness features, self-renewal capacity, and Wnt-related organoid formation  J Biomed Sci 27, 32 (2020). https://doi.org/10.1186/s12929-019-0602-1
  11. F. Sato, et al. Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis Clocks & Sleep 2020, 2, 26–38; doi:10.3390/clockssleep2010004
  12. R. Sarfstein, et al. Differential Effects of Insulin and IGF1 Receptors on ERK and AKT Subcellular Distribution in Breast Cancer Cells Cells 2019, 8(12), 1499; https://doi.org/10.3390/cells8121499
  13. A. Paul, et al. Tryptophan-Glucosamine Conjugates Modulate Tau-Derived PHF6 aggregation at Low concentrations Chemical Communications, 12 Nov 2019, 10.1039/C9CC06868F
  14. M. Sammar, et al. Correlation between cytotoxicity in cancer cells and free radical‑scavenging activity: In vitro evaluation of 57 medicinal and edible plant extracts Oncology Letters, November 5, 2019 https://doi.org/10.3892/ol.2019.11054 
  15. S.K. Hsieh, et al. Promotion of myotube differentiation and attenuation of muscle atrophy in murine C2C12 myoblast cells treated with teaghrelin  Chemico-Biological Interactions, Volume 315, 5 January 2020, 108893, https://doi.org/10.1016/j.cbi.2019.108893
  16. A. Khattib, et al. Lyso‐diacylglyceryltrimethylhomoserine (lyso‐DGTS) isolated from Nannochloropsis microalgae improves high‐density lipoprotein (HDL) functions International Union of Biochemistry and Molecular Biology, 29 October 2019 https://doi.org/10.1002/biof.1580
  17. N. Reuven et al., Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing Biomolecules 2019, 9, 584; doi:10.3390/biom9100584
  18. Y. C. Kuo, et al. Self-assembled ternary poly(vinyl alcohol)-alginate-gelatin hydrogel with controlled-release nanoparticles for pancreatic differentiation of iPS cells Journal of the Taiwan Institute of Chemical Engineers, 23 September 2019, https://doi.org/10.1016/j.jtice.2019.09.010
  19. S. Yalcin Dextran-coated iron oxide nanoparticle for delivery of miR-29a to breast cancer cell line. Pharmaceutical Development and Technology, volume 24, 2019 - Issue 8
  20. S. Hoffman, et al. Faecal Proteases from Pouchitis Patients Activate Protease Activating Receptor-2 to Disrupt the Epithelial Barrier Journal of Crohn's and Colitis, jjz086, https://doi.org/10.1093/ecco-jcc/jjz086, 06 May 2019
  21. P. Şenel et al. Methyl substituent effect on one‐dimensional copper(II) coordination polymers containing biologically active ligands: Synthesis, characterization, DNA interactions and cytotoxicities Applied Organometallic Chemistry, 24 July 2019, https://doi.org/10.1002/aoc.5122
  22. A. Synowiec et al. Cat flu: Broad spectrum polymeric antivirals Antiviral Research, Volume 170, October 2019, 104563 
  23. L. Achlaug et al. Identification of ZYG11A as a candidate IGF1-dependent proto-oncogene in endometrial cancer. Oncotarget, 2019 Jul 8, 10(43): 4437-4448, 2019 Jul 8, doi: 10.18632/oncotarget.27055
  24. F. Sato et al. Differential expression of claudin‑4, occludin, SOX2 and proliferating cell nuclear antigen between basaloid squamous cell carcinoma and squamous cell carcinoma Molecular Medicine Reports, June 24, 2019 https://doi.org/10.3892/mmr.2019.10417, Pages:1977-1985
  25. Y. Serap, Dextran-coated iron oxide nanoparticle for delivery of miR-29a to breast cancer cell line Pharmaceutical Development and Technology, Pages 1032-1037, 21 Jun 2019
  26. K. C. Huang et al. Hyperglycemia inhibits osteoblastogenesis of rat bone marrow stromal cells via activation of the Notch2 signaling pathway International Journal of Medical Sciences 2019; 16(5): 696-703. doi: 10.7150/ijms.32707
  1. S. Hoffman et al. Faecal Proteases from Pouchitis Patients Activate Protease Activating Receptor-2 to Disrupt the Epithelial Barrier  Journal of Crohn's and Colitis, jjz086, https://doi.org/10.1093/ecco-jcc/jjz086, 06 May 2019
  2. S. Kiraz et al. Antiproliferative activity of copper(II) glutamine complexes with N,N-donor ligands: Synthesis, characterization, potentiometric studies and DNA/BSA interactions. Journal of Molecular Structure, Available online 25 May 2019
  3. S. Monga et al. Inhibitory Effects of the Two Novel TSPO Ligands 2-Cl-MGV-1 and MGV-1 on LPS-induced Microglial Activation. Cells 2019, 8, 486; doi:10.3390/cells8050486
  4. A. Cetin et al. In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines. Czech Journal of Food Sciences, 37, 2019 (1): 0–7
  5. C. C. Chang et al. Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression. International Journal of Medical Sciences, 2019; 16(1): 167-179. doi: 10.7150/ijms.24068
  6. E. Tanış et al. Synthesis, Experimental and Theoretical Analysis, and Antiproliferative Activity of 2-(4-methoxyphenylamino)-2-oxoethyl methacrylate. Chinese Journal of Physics, Available online 22 November 2018
  7. D. İnci et al. Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: Synthesis, X‐ray crystal structures, biomolecular interactions, antioxidant activity, ROS generation and cytotoxicity. Applied Organometallic Chemistry, 2018
  8. S. Izraely et al. The Metastatic Microenvironment: Melanoma‐Microglia Cross‐Talk Promotes the Malignant Phenotype of Melanoma Cells. International Journal of Cancer, 11 July 2018 https://doi.org/10.1002/ijc.31745
  9. A. Szczepanski et al. Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells. Veterinary Research201849:55
  10. Hironori Bando, Yutaka Takahashi et al. Isolated adrenocorticotropic hormone deficiency as a form of paraneoplastic syndrome. Pituitary (2018). https://doi.org/10.1007/s11102-018-0901-7
  11. Y.C. Chen et al. Suppressive effects of metformin on T-helper 1-related chemokines expression in the human monocytic leukemia cell line THP-1. Endocrine Research, 2018
  12. D. İnci et al. Newly synthesized Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes as potential anticancer candidates. Applied Organometallic Chemistry, volume32, Issue4, April 2018
  13. D. Witt-Kehati et al. Inhibition of pMAPK14 Overcomes Resistance to Sorafenib in Hepatoma Cells with Hepatitis B Virus1. Translational Oncology, volume 11, Issue 2, April 2018, pages 511-517
  14. Y.H. Chang et al. Human umbilical cord-derived mesenchymal stem cells reduce monosodium iodoacetate-induced apoptosis in cartilage. Tzu Chi medical Journal, Year : 2018 | Volume : 30 | Issue : 2 | Page : 71-80
  15. V.B. Kumar at al. Ultrafine Highly Magnetic Fluorescent γ-Fe2O3/NCD Nanocomposites for Neuronal Manipulations. ACS Omega 2018 3 (2), 1897-1903
  16. Y.S Chen et al. Characterizing the differentiation of osteoprogenitor cells on surface modified polyether-ether-ketone. Surface and Coatings Technology, 2018
  17. C.W. Chang et al. In Vitro Evaluation of the Antiviral Activity of Some Mushrooms from Turkey. International Journal of Medicinal Mushrooms, issue 3, pages 201-212, 2018
  18. Y.C. Kuo et al. Use of functionalized liposomes loaded with antioxidants to permeate the blood–brain barrier and inhibit β-amyloid-induced neurodegeneration in the brain. Journal of the Taiwan Institute of Chemical Engineers. Volume 87, June 2018, Pages 1-14
  19. M. Bar-Zeev at al. Re-assembled casein micelles for oral delivery of chemotherapeutic combinations to overcome multi drug resistance in gastric cancer. Journal of Molecular and Clinical Medicine, Vol. 1 | Issue 1 | January 2018
  20. G. E. Akbaba et al. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. Marmara Pharmaceutical Journal . 2018, Vol. 22 Issue 2, p299-306. 8p.

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Reviews

Customer Reviews (2)

Trustworthy!Review by Raghavendra Basavaraja
Quality
Simplest and most reliable kit for quantifying viable cell numbers. (Posted on 1/27/2018)
ExcellentReview by Orit Sagi-Assif
Quality
The best kit (Posted on 1/9/2018)

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