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BME Medium, Earle's salts, (10X)

No L-Glutamine, no Sodium Bicarbonate, 500 mL
SKU: 01-015-5A



Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

BME contains eight B vitamins, ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

This BME is modified as follows:


  • Earle’s Salts Base
  • Phenol Red


  • L-Glutamine
  • Sodium Bicarbonate

The complete formulation is available.

See here instructions for the preparation of single strength synthetic liquid media (1X) from concentrated media



Glutamine No Glutamine
QTY 500 mL
Phenol Red Indicator Phenol Red
Storage Conditions 2-8°C
Form Liquid
Sodium Bicarbonate Buffer no Sodium Bicarbonate


Certificate of Analysis

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