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Description
Details
Product Overview:
BIOMYC™-1 Antibiotic Solution is a treatment based on the antibiotic Tiamutin. BIOMYC-1 when used with BIOMYC-2, a treatment solution based on the antibiotic Minocycline, which is a member of the Tetracycline group, has been shown to be effective in eliminating the mycoplasma species frequently present in contaminated cell cultures. Mycoplasmas do not develop resistance to these antibiotics – a common occurrence with other antibiotic treatment methods.
This treatment is based on alternating BIOMYC-1 and BIOMYC-2.
Recommended Use:
Tip: Four days with BIOMYC-1 (Tiamutin) followed by three days with BIOMYC-2 (Minocycline) then repeating the cycle three times.- Do not use the two solutions together, but rather sequentially.
- Add 1ml BIOMYC-1 to 100ml medium, and maintain the contaminated cells in this mixture for 4 days. Any fresh medium added should also contain BIOMYC-1.
- After 4 days, add 1ml BIOMYC-2 to 100ml fresh medium, and maintain the cells in this second mixture for 3 days.
- The above, together, are considered as one treatment cycle. It may be necessary to repeat this cycle 2-3 times.
- During the process, the cells can be tested for mycoplasma contamination, and results can then be used to shorten the process when possible.
Please note: On completion of the treatment, the cells should be grown on media without antibiotics for at least two weeks and then re-tested for the presence of mycoplasma. If the culture tests positive, the course of treatment should be repeated.
Specifications
Specifications
| Brand | BIOMYC™ |
|---|---|
| Storage Conditions | -20º |
| Shipping Conditions | Dry Ice |
Documentation
Materials Safety Data Sheet
Manuals and Protocols
Certificate of Analysis
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