Stem Cell Media Selection Guide

Which stem cell culture medium is right for you?

Medium

NutriStem® hPSC XF Medium
NutriStem® hPSC XF Medium, Modified GF-Free

NutriStem® V9 XF Medium

MSC NutriStem® XF Medium
Cell Type Human PSCs Human PSCs Human PSCs Human PSCs Human PSCs Human PSCs Human MSCs
Application Feeder-Dependent hPSC culture Feeder-Free
hPSC culture
Translational hPSC culture Translational hPSC culture, single cell Reprogramming hPSC culture on vitronectin Completely Defined Translational hMSC culture
Additional Supplement None None None None bFGF (20 ng/mL) NutriStem® V9 ACF Supplement Mix MSC NutriStem® Supplement Mix
Matrix MEFs/HFFs Matrigel
Cultrex
Geltrex
Other
LaminStem™ 521
Laminin-511
LaminStem™ 521 LaminStem™ 521
Matrigel
Laminin-511
Vitronectin MSC Attachment Solution
Xeno-Free Possible (HFFs) No Yes Yes Yes Yes Yes
Weekend-Free Feeding* No Yes Yes Yes n/a Yes n/a
Dissociation Reagent Collagenase IV Dispase
Collagenase IV
Accutase
0.5mM EDTA
0.5mM EDTA
Accutase
Trypsin-EDTA
Accutase
Trypsin-EDTA
Accutase
0.5mM EDTA Recombinant Trypsin Solution
Freezing Media CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
CryoStem™
Freezing Medium
MSC Freezing Solution
Additional Reagents Gelatin, Human Serum Albumin (HSA), Soybean Trypsin Inhibitor, EZ-PCR™ Mycoplasma Detection Kit, DPBS, Aquaguard™-1 Solution, Aquaguard™-2 Solution for Water Baths

*Weekend-free feeding is possible, though with any hPSC culture, optimal growth is seen with daily feeding. It is not recommended to skip feedings on a routine or long-term basis.